US4645646A
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Luminescence immunoassay for haptens and chemiluminescence labelled hapten conjugates
Assignee: Henning Berlin GmbH Chemie- und Pharmawerk
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Filing: 1984-07-27
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Granted: 1987-02-24
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Expires: 2004-07-27
Abstract
Abstract Luminescence immunoassays for haptens can be improved and made more sensitive by using a luminescence labelled hapten conjugate which contains as the linkage group a chain-like polymer having repeating functional groups having bound thereto per mole both a plurality of moles of groups capable of luminescence and a plurality of moles of hapten. The antibody used is preferably one which is prepared by the use of another chain-like polymer having bound thereto the hapten by a different chemical reaction.
Inventors
- 👤 Andre Gadow
- 👤 W. Graham Wood
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 03:19
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/543; G01N33/544; G01N33/545; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
83 words
A test kit for hapten luminescence immunoassay consisting of as a merchantile unit (A) an antibody specific to a hapten, (B) a chemiluminescence labelled hapten conjugate including (a) a plurality of groups displaying chemiluminescence, (b) a linkage group, and (c) a plurality of haptens, said linkage groups (b) being a chain-like polymer having repeating functional units and having bound thereto said plurality of groups displaying chemiluminescence (a) and said plurality of haptens (c), and (C) directions for carrying out said hapten luminescence immunoassy.
Claim 2
Dependent Claim
83 words
A test kit for hapten luminescence immunoassay consisting of as a merchantile unit (A) an antibody specific to a hapten, (B) a chemiluminescence labelled hapten conjugate including (a) a plurality of groups displaying chemiluminescence, (b) a linkage group, and (c) a plurality of haptens, said linkage groups (b) being a chain-like polymer having repeating functional units and having bound thereto said plurality of groups displaying chemiluminescence (a) and said plurality of haptens (c), and (C) directions for carrying out said hapten luminescence immunoassy.
Claim 3
Dependent Claim
28 words
A composition according to claim 1, wherein the antibody (A) was prepared using as the immunogen a hapten bound to a linkage group other than linkage group (b).
Claim 4
Dependent Claim
22 words
A test kit according to claim 1, wherein the linkage group (b) is a peptide, a glycoprotein, a glycolipid or a carbohydrate.
Claim 5
Dependent Claim
31 words
A test kit according to claim 1, wherein the molar ratio of the linkage group (b) to the group (a) capable of chemiluminescence to the hapten (c) is at least 1:10:10.
Claim 6
Dependent Claim
31 words
A test kit according to claim 3, wherein the molar ratio of the linkage group (b) to the group (a) capable of chemiluminescence to the hapten (c) is at least 1:10:10.
Claim 7
Dependent Claim
25 words
A test kit according to claim 1, wherein the antibody (A) which is specific to the hapten is present bound to a solid supporting material.
Claim 8
Dependent Claim
25 words
A test kit according to claim 5, wherein the antibody (A) which is specific to the hapten is present bound to a solid supporting material.
Claim 9
Dependent Claim
51 words
A chemiluminescence-labelled hapten conjugate comprising (a) a plurality of groups displaying chemiluminescence, (b) a linkage group, and (c) a plurality of haptens, said linkage group (b) being a chain-like polymer having repeating functional units and having bound thereto said plurality of groups displaying chemiluminescence (a) and said plurality of haptens (c).
Claim 10
Dependent Claim
51 words
A chemiluminescence-labelled hapten conjugate comprising (a) a plurality of groups displaying chemiluminescence, (b) a linkage group, and (c) a plurality of haptens, said linkage group (b) being a chain-like polymer having repeating functional units and having bound thereto said plurality of groups displaying chemiluminescence (a) and said plurality of haptens (c).
Claim 11
Dependent Claim
22 words
Labelled hapten conjugate according to claim 8, wherein the linkage group (b) is a peptide, a glycoprotein, a glycolipid or a carbohydrate.
Claim 12
Dependent Claim
31 words
Labelled hapten conjugate according to claim 9, wherein the molar ratio of the linkage group (b) to the group (a) capable of luminescence to the hapten (c) is at least 1:10:10.
Claim 13
Dependent Claim
15 words
The test kit of claim 1 wherein said linkage group (b) is transferrin or thyroglobulin.
Claim 14
Dependent Claim
16 words
The chemiluminescence-labeled hapten conjugate of claim 8 wherein said linkage group (b) is transferrin or thyroglobulin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1990-02-23 | 1990-08-25 | 1991-02-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-02-23 | 1994-08-25 | 1995-02-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-02-23 | 1998-08-25 | 1999-02-24 | $7700.00 | Window Closed / Lapsed |