EP0323692A2
Expired / Lapsed
EP
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Water-insoluble reagent, elements containing same and methods of use
Assignee: Eastman Kodak Co
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Filing: 1988-08-03
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Granted: —
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Expires: 2008-08-03
Abstract
Abstract A water-insoluble immunoreactive reagent is prepared from a polymeric particle composed of a polymer derived from at least one ethylenically unsaturated polymerizable monomer having either pendant activated 2-substituted ethylsulfonyl or vinylsulfonyl groups. The interior of the particle is substantially free of detectable tracer material. The particle is covalently attached through the pendant groups to an immunological species which is capable of participating in an immunological reaction to complex with a corresponding receptor. This immunoreactive reagent can be incorporated in elements for use in immunoassays. In addition, they can be used in various immunological methods, including agglutination, sandwich and competitive binding assays.
Inventors
- 👤 Richard Calvin Eastman Kodak Co. Sutton
- 👤 Susan Jean Eastman Kodak Co. Danielson
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 18:37
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G01N33/54313
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
70 words
A reagent composed of: (a) a polymeric particle covalently attached through reactive pendant groups to (b) an immunological species which is capable of participating in an immunological reaction with a corresponding receptor, the reagent characterized wherein the polymer is derived from at least one ethylenically unsaturated polymerizable monomer having either pendant activated 2-substituted ethylsulfonyl or vinylsulfonyl groups, and the interior of the particle being substantially free of detectable tracer material.
Claim 2
Dependent Claim
70 words
A reagent composed of: (a) a polymeric particle covalently attached through reactive pendant groups to (b) an immunological species which is capable of participating in an immunological reaction with a corresponding receptor, the reagent characterized wherein the polymer is derived from at least one ethylenically unsaturated polymerizable monomer having either pendant activated 2-substituted ethylsulfonyl or vinylsulfonyl groups, and the interior of the particle being substantially free of detectable tracer material.
Claim 3
Dependent Claim
178 words
The reagent as claimed in claim 1 wherein the polymer is represented by the formula: wherein A represents recurring units derived from one or more hydrophobic ethylenically unsaturated monomers, B represents recurring units derived from one or more ethylenically unsaturated monomers represented by the formula: wherein R is hydrogen or substituted or unsubstituted alkyl, R¹ is -CH=CHR² or -CH₂CH₂ X wherein X is a leaving group which is displaced by a nucleophile or is eliminated in the form of HX by treatment with a base, and R² is hydrogen, substituted or unsubstituted alkyl, or substituted or unsubstituted aryl, and L is a linking group selected from the group consisting of substituted or unsubstituted alkylene, substituted or unsubstituted arylene, and a combination of one or more of each of the alkylene and arylene groups, D represents recurring units derived from one or more ethylenically unsaturated monomers other than those represented by A or B, x is from 0 to 99.9 mole percent, y is from 0.1 to 100 mole percent, and z is from 0 to 20 mole percent.
Claim 4
Dependent Claim
37 words
The reagent as claimed in claim 2 wherein x is from 50 to 99.5 mole percent, y is from 0.5 to 50 mole percent, and z is from 0 to 10 mole percent in the defined polymer.
Claim 5
Dependent Claim
40 words
The reagent as claimed in either of claims 2 or 3 wherein the B recurring units are derived from the defined monomers wherein R is hydrogen or methyl, R¹ is -CH₂CH₂X and L is substituted or unsubstituted phenylenealkylene or carbonyliminomethyleneiminocarbonylethylene.
Claim 6
Dependent Claim
53 words
The reagent as claimed in any of claims 1 to 4 wherein the immunological species is an antibody directed against digoxin, phenytoin, phenobarbital, thyroxine, triiodothyronine, gentamicin, carbamazepine, Primidone, tobramycin, theophylline, human chorionic gonadotropin, leutinizing hormone, a chlamydial antigen, a gonococcal antigen, a herpes antigen, a retroviral antigen or antibody or a Streptococcal antigen.
Claim 7
Dependent Claim
32 words
The reagent as claimed in any of claims 1 to 4 wherein the immunological species is a retroviral, herpes, chlamydial or gonococcal organism or antigenic component thereof, or a drug or hapten.
Claim 8
Dependent Claim
35 words
An element comprising an absorbent carrier material having one or more zones, the element characterized as containing in one or more of the zones a reagent as claimed in any of claims 1 to 6.
Claim 9
Dependent Claim
12 words
The element as claimed in claim 7 further comprising a ligand analog.
Claim 10
Independent Claim
77 words
A method for the determination of an immunological ligand in an aqueous fluid comprising: A. in the presence of an analog of the ligand, contacting a sample of the fluid with a reagent as claimed in any of claims 1 to 6, to form an immunological complex between the immunological species and both the immunological ligand and ligand analog, and B. determining the immunological complex as an indication of the presence of the ligand in the fluid.
Claim 11
Dependent Claim
38 words
The method as claimed in claim 9 wherein the ligand analog is labeled with an enzyme, and the method is carried out in the presence of reagents which will react with the enzyme to provide a detectable dye.
Claim 12
Dependent Claim
70 words
An agglutination method for the determination of a ligand in an aqueous liquid comprising: A. contacting the liquid with a reagent as claimed in any of claims 1 to 6, so as to form an agglutinate of the reaction product of the ligand and the immunological species, B. separating the agglutinate from unagglutinated materials, and C. determining the agglutinate as an indication of the presence of ligand in the liquid.
Claim 13
Independent Claim
118 words
A method for the determination of a ligand in an aqueous liquid comprising: A. contacting the liquid with a reagent as claimed in any of claims 1 to 6, so as to form an insoluble immunological complex between the immunological species and the ligand, B. prior to, simultaneously with, or subsequent to contacting step A, contacting the ligand with a second immunological species which is immunologically reactive with the ligand but which is not immunologically reactive with the first immunological species, the second species being labeled with a detectable tracer material, so as to form a labeled insoluble complex, and C. determining the labeled insoluble complex as an indication of the presence of the ligand in the liquid.
Claim 14
Dependent Claim
25 words
The method as claimed in claim 12 wherein the ligand is an antigen and the first and second immunological species are antibodies against the antigen.
Claim 15
Dependent Claim
36 words
The method as claimed in claim 12 wherein the ligand is an antibody, the first immunological species is an antigen reactive with the ligand, and the second immunological species is an antibody directed against the ligand.
References Cited (Backward Citations)
No citation records indexed.
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