US5541309A
Expired / Lapsed
US
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Modified apoaequorin having increased bioluminescent activity
Assignee: Woods Hole Oceanographic Institution
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Filing: 1994-10-28
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Granted: 1996-07-30
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Expires: 2014-10-28
Abstract
Abstract The present invention encompasses modified apoaequorin nucleotide and amino acid sequences capable of emitting light in the presence of luciferin and a light-triggering cation such as Ca2+, which possess bioluminescent activity greater than unmodified apoaequorin and methods of use therefore.
Inventors
- 👤 Douglas Prasher
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 15:51
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/43504; C07K14/43595; Y; Y10; Y10S; Y10S530/00; Y10S530/855
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
79 words
A composition comprising an isolated altered apoaequorin DNA sequence, wherein at least the codon that codes for an aspartic acid at amino acid position 124 in the amino acid sequence of natural apoaequorin is changed to a codon that codes for serine, wherein said isolated altered apoaequorin DNA sequence codes for an altered apoaequorin which, when combined with a luciferin and a light-triggering cation, has greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 2
Dependent Claim
79 words
A composition comprising an isolated altered apoaequorin DNA sequence, wherein at least the codon that codes for an aspartic acid at amino acid position 124 in the amino acid sequence of natural apoaequorin is changed to a codon that codes for serine, wherein said isolated altered apoaequorin DNA sequence codes for an altered apoaequorin which, when combined with a luciferin and a light-triggering cation, has greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 3
Dependent Claim
15 words
The composition of claim 1 wherein the altered apoaequorin DNA is SEQ ID NO: 8.
Claim 4
Dependent Claim
79 words
A composition comprising an isolated altered apoaequorin DNA sequence, wherein at least the codon that codes for a glutamic acid at amino acid position 135 in the amino acid sequence of natural apoaequorin is changed to a codon that codes for serine, wherein said isolated altered apoaequorin DNA sequence codes for an altered apoaequorin which, when combined with a luciferin and a light-triggering cation, has greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 5
Dependent Claim
79 words
A composition comprising an isolated altered apoaequorin DNA sequence, wherein at least the codon that codes for a glutamic acid at amino acid position 135 in the amino acid sequence of natural apoaequorin is changed to a codon that codes for serine, wherein said isolated altered apoaequorin DNA sequence codes for an altered apoaequorin which, when combined with a luciferin and a light-triggering cation, has greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 6
Dependent Claim
15 words
The composition of claim 3 wherein the altered apoaequorin DNA is SEQ ID NO: 9.
Claim 7
Dependent Claim
78 words
A composition comprising an isolated altered apoaequorin DNA sequence, wherein at least the codon that codes for a glycine at amino acid position 129 in the amino acid sequence of natural apoaequorin is changed to a codon that codes for alanine, wherein said isolated altered apoaequorin DNA sequence codes for an altered apoaequorin which, when combined with a luciferin and a light-triggering cation, has greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 8
Dependent Claim
78 words
A composition comprising an isolated altered apoaequorin DNA sequence, wherein at least the codon that codes for a glycine at amino acid position 129 in the amino acid sequence of natural apoaequorin is changed to a codon that codes for alanine, wherein said isolated altered apoaequorin DNA sequence codes for an altered apoaequorin which, when combined with a luciferin and a light-triggering cation, has greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 9
Dependent Claim
15 words
The composition of claim 5 wherein the altered apoaequorin DNA is SEQ ID NO: 10.
Claim 10
Dependent Claim
75 words
A composition comprising an isolated, altered, apoaequorin DNA sequence, wherein at least the codon that codes for an aspartic acid at codon position 117 of SEQ ID NO: 7 is changed to a codon that codes for serine, wherein said isolated altered apoaequorin DNA sequence codes for an altered apoaequorin which, when combined with a luciferin and a light-triggering cation, has greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 11
Dependent Claim
75 words
A composition comprising an isolated, altered, apoaequorin DNA sequence, wherein at least the codon that codes for an aspartic acid at codon position 117 of SEQ ID NO: 7 is changed to a codon that codes for serine, wherein said isolated altered apoaequorin DNA sequence codes for an altered apoaequorin which, when combined with a luciferin and a light-triggering cation, has greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 12
Dependent Claim
75 words
A composition comprising an isolated altered apoaequorin DNA sequence, wherein at least the codon that codes for a glutamic acid at codon position 128 of SEQ ID NO: 7 is changed to a codon that codes for serine, wherein said isolated altered apoaequorin DNA sequence codes for an altered apoaequorin which, when combined with a luciferin and a light-triggering cation, has greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 13
Dependent Claim
75 words
A composition comprising an isolated altered apoaequorin DNA sequence, wherein at least the codon that codes for a glutamic acid at codon position 128 of SEQ ID NO: 7 is changed to a codon that codes for serine, wherein said isolated altered apoaequorin DNA sequence codes for an altered apoaequorin which, when combined with a luciferin and a light-triggering cation, has greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 14
Dependent Claim
82 words
A composition comprising an isolated altered apoaequorin DNA sequence, wherein at least the codon that codes for a glycine at codon position 122 of SEQ ID NO: 7 in the amino acid sequence of natural apoaequorin is changed to a codon that codes for alanine, wherein said isolated altered apoaequorin DNA sequence codes for an altered apoaequorin which, when combined with a luciferin and a light-triggering cation, has greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 15
Dependent Claim
82 words
A composition comprising an isolated altered apoaequorin DNA sequence, wherein at least the codon that codes for a glycine at codon position 122 of SEQ ID NO: 7 in the amino acid sequence of natural apoaequorin is changed to a codon that codes for alanine, wherein said isolated altered apoaequorin DNA sequence codes for an altered apoaequorin which, when combined with a luciferin and a light-triggering cation, has greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-07-30 | 2000-01-29 | 2000-07-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-07-30 | 2004-01-29 | 2004-07-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-07-30 | 2008-01-29 | 2008-07-30 | $7700.00 | Window Closed / Lapsed |