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US5288623A
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Process for secretory production of a calcium-binding protein
Assignee: JNC Corp
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Filing: 1992-07-13
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Granted: 1994-02-22
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Expires: 2012-07-13
Abstract
Abstract A process for secretory production of a calcium-binding protein very useful for producing heterogenic proteins in Escherichia coli according to gene recombinant technique is provided, which process comprises using Escherichia coli and using a secretory expression system with the promoter of lipoprotein and the gene of signal peptide of outer membrane protein A, according to recombinant DNA technique.
Inventors
- 👤 Shuhei Zenno
- 👤 Satoshi Inouye
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 16:22
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/195; C07K14/305; C07K14/31; C07K14/435; C07K14/43504; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2468; C12N9/2471; C12Y; C12Y302/00; C12Y302/01; C12Y302/01023; C07K2319/00; C07K2319/01; C07K2319/02
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
172 words
A process for producing a fusion protein comprising a calcium-binding protein and biogenic substance of E. coli which facilitates the extracellular secretory production of the fusion protein, which process comprises: preparing a recombinant plasmid as a cloning vehicle for expression of the fusion protein in E. coli, said plasmid comprising a first DNA sequence consisting of a lipoprotein promoter region being linked in reading phase with a second DNA sequence located downstream of said first DNA sequence, said second DNA sequence coding for a signal peptide of outer membrane protein A and being linked in reading phase with a third DNA sequence located downstream of said second DNA sequence, said third DNA sequence coding for the fusion protein, inserting said plasmid into E. coli to obtain a transformed E. coliand cultivating the transformed E. coli in a suitable medium to produce said fusion protein, wherein said calcium-binding protein is selected from the group consisting of calmodulin, troponin c, myosin light chain, parvalubumin, vitamin D-dependent calcium-binding proteins, S-100, S-100β, calpactin, carpaine/CANP, and oncomodulin.
Claim 2
Dependent Claim
172 words
A process for producing a fusion protein comprising a calcium-binding protein and biogenic substance of E. coli which facilitates the extracellular secretory production of the fusion protein, which process comprises: preparing a recombinant plasmid as a cloning vehicle for expression of the fusion protein in E. coli, said plasmid comprising a first DNA sequence consisting of a lipoprotein promoter region being linked in reading phase with a second DNA sequence located downstream of said first DNA sequence, said second DNA sequence coding for a signal peptide of outer membrane protein A and being linked in reading phase with a third DNA sequence located downstream of said second DNA sequence, said third DNA sequence coding for the fusion protein, inserting said plasmid into E. coli to obtain a transformed E. coliand cultivating the transformed E. coli in a suitable medium to produce said fusion protein, wherein said calcium-binding protein is selected from the group consisting of calmodulin, troponin c, myosin light chain, parvalubumin, vitamin D-dependent calcium-binding proteins, S-100, S-100β, calpactin, carpaine/CANP, and oncomodulin.
Claim 3
Dependent Claim
144 words
A process for producing a fusion protein comprising apoaequorin and a biogenic substance in E. coli which facilitates the extracellular secretory production of the fusion protein, which process comprises: preparing a recombinant plasmid as a cloning vehicle for expression of the fusion protein in E. coli, said plasmid comprising a first DNA sequence consisting of a lipoprotein promoter region being linked in reading phase with a second DNA sequence located downstream of said first DNA sequence, said second DNA sequence coding for a signal peptide of outer membrane protein A and being linked in reading phase with a third DNA sequence located downstream of said second DNA sequence, said third DNA sequence coding for the fusion protein, inserting said plasmid into E. coli to obtain a transformed E. coli, and cultivating the transformed E. coli in a suitable medium to produce said fusion protein.
Claim 4
Dependent Claim
144 words
A process for producing a fusion protein comprising apoaequorin and a biogenic substance in E. coli which facilitates the extracellular secretory production of the fusion protein, which process comprises: preparing a recombinant plasmid as a cloning vehicle for expression of the fusion protein in E. coli, said plasmid comprising a first DNA sequence consisting of a lipoprotein promoter region being linked in reading phase with a second DNA sequence located downstream of said first DNA sequence, said second DNA sequence coding for a signal peptide of outer membrane protein A and being linked in reading phase with a third DNA sequence located downstream of said second DNA sequence, said third DNA sequence coding for the fusion protein, inserting said plasmid into E. coli to obtain a transformed E. coli, and cultivating the transformed E. coli in a suitable medium to produce said fusion protein.
References Cited (Backward Citations)
US4595658A
Cited
US4839293A
Cited
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