US5162227A
Expired / Lapsed
US
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Recombinant DNA vectors capable of expressing apoaequorin in E. coli
Assignee: University of Georgia Research Foundation Inc
•
Filing: 1988-03-17
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Granted: 1992-11-10
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Expires: 2008-03-17
Abstract
Abstract A gene which codes for the protein apoaequorin is disclosed along with recombinant DNA vectors containing this gene.
Inventors
- 👤 Milton J. Cormier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 23:24
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/43504; C07K14/43595
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
17 words
An isolated DNA molecule, which comprises a nucleotide sequence coding for a peptide of the formula ##STR9##
Claim 2
Dependent Claim
17 words
An isolated DNA molecule, which comprises a nucleotide sequence coding for a peptide of the formula ##STR9##
Claim 3
Dependent Claim
10 words
The molecule of claim 1, wherein said sequence is ##STR10##
Claim 4
Dependent Claim
30 words
The molecule of claim 2 wherein said molecule further comprises a non-expressed segment 3' to said sequence and a non-expressed segment 5' to said sequence, wherein said molecule is ##STR11##
Claim 5
Dependent Claim
11 words
The molecule of claim 2, wherein said molecule is single-stranded DNA.
Claim 6
Dependent Claim
11 words
The molecule of claim 2, wherein said molecule is double-stranded DNA.
Claim 7
Dependent Claim
25 words
A recombinant DNA vector capable of reproducing in a microorganism, wherein said vector comprises a nucleotide sequence coding for a peptide of the formula ##STR12##
Claim 8
Dependent Claim
25 words
A recombinant DNA vector capable of reproducing in a microorganism, wherein said vector comprises a nucleotide sequence coding for a peptide of the formula ##STR12##
Claim 9
Dependent Claim
10 words
The vector of claim 6, wherein said sequence is ##STR13##
Claim 10
Dependent Claim
14 words
The vector of claim 6, wherein said sequence is preceded by a lac promotor.
Claim 11
Dependent Claim
15 words
A genetically engineered E. coli microorganism wherein said microorganism comprises the vector of claim 6.
Claim 12
Dependent Claim
16 words
A microorganism of claim 9, wherein said vector comprises a nucleotide sequence of the formula ##STR14##
References Cited (Backward Citations)
US4604364A
Cited
US4396579A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-11-10 | 1996-05-11 | 1996-11-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-11-10 | 2000-05-11 | 2000-11-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-11-10 | 2004-05-11 | 2004-11-10 | $7700.00 | Window Closed / Lapsed |