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US5523388A
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Methods of synthesizing oligonucleotides with random codons
Assignee: Applied Molecular Evolution Inc
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Filing: 1995-02-27
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Granted: 1996-06-04
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Expires: 2015-02-27
Abstract
Abstract The invention provides a method of synthesizing oligonucleotides having random tuplets using individual monomers. The steps consist of: (1) sequentially coupling monomers on separate supports to form at least two different tuplets, the coupling is performed in separate reaction vessels; (2) mixing the supports from the reaction vessels; (3) dividing the mixed supports into two or more separate reaction vessels; and (4) repeating steps (1) through (3) one or more times in the reaction vessels of step (3), wherein the last step ends at step (2). Additionally, the oligonucleotides can be cleaved from the supports.
Inventors
- 👤 William D. Huse
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 18:50
CPC Classifications
C; C07; C07H; C07H21/00
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
91 words
A method of synthesizing oligonucleotides having random tuplets using individual monomers comprising the steps of: (1) sequentially coupling monomers on separate supports to form at least two different tuplets, said coupling being performed in separate reaction vessels; (2) mixing the supports from the separate reaction vessels; (3) dividing the mixed supports into two or more separate reaction vessels; and (4) repeating steps (1) through (3) one or more times in the reaction vessels of step (3), wherein the last step ends at step (2); wherein said tuplets are not triplet codons.
Claim 2
Independent Claim
91 words
A method of synthesizing oligonucleotides having random tuplets using individual monomers comprising the steps of: (1) sequentially coupling monomers on separate supports to form at least two different tuplets, said coupling being performed in separate reaction vessels; (2) mixing the supports from the separate reaction vessels; (3) dividing the mixed supports into two or more separate reaction vessels; and (4) repeating steps (1) through (3) one or more times in the reaction vessels of step (3), wherein the last step ends at step (2); wherein said tuplets are not triplet codons.
Claim 3
Dependent Claim
18 words
The method of claim 1 further comprising step (5) cleaving said oligonucleotides with random tuplets from said support.
Claim 4
Dependent Claim
18 words
The method of claim 1, wherein the number of tuplets to be randomized is between two and twenty.
Claim 5
Dependent Claim
21 words
The method of claim 1, wherein the number of reaction vessels is equal to the number of tuplets to be randomized.
Claim 6
Dependent Claim
18 words
The method of claim 4, wherein each tuplet to be randomized is coupled in a separate reaction vessel.
Claim 7
Dependent Claim
21 words
The method of claim 1, wherein the number of reaction vessels is less than the number of tuplets to be randomized.
Claim 8
Dependent Claim
20 words
The method of claim 6, wherein two tuplets to be randomized are coupled to a support in a reaction vessel.
Claim 9
Dependent Claim
49 words
A method of synthesizing oligonucleotides using individual monomers having at least one specified tuplet at a predetermined position and the remaining positions having random tuplets comprising the method of claim 1, wherein said specified tuplet position is synthesized on the mixed supports prior to dividing into separate reaction vessels.
Claim 10
Dependent Claim
21 words
The method of claim 8, wherein the first position is predetermined and the specified tuplet is synthesized prior to step (1).
Claim 11
Independent Claim
112 words
A method of synthesizing oligonucleotides having tuplets which are diverse but biased toward a predetermined sequence comprising the steps of: (1) sequentially coupling monomers on a support to form a tuplet with a predetermined sequence, said coupling being performed in a first reaction vessel; (2) sequentially coupling monomers on a support to form a random tuplet, said coupling being performed in a second reaction vessel; (3) mixing the supports from said first and second reaction vessels; and (4) dividing the mixed supports from step (3) and using the supports in step (1) and step (2), wherein steps (1) through (3) are repeated at least once; wherein said tuplets are not triplet codons.
Claim 12
Independent Claim
112 words
A method of synthesizing oligonucleotides having tuplets which are diverse but biased toward a predetermined sequence comprising the steps of: (1) sequentially coupling monomers on a support to form a tuplet with a predetermined sequence, said coupling being performed in a first reaction vessel; (2) sequentially coupling monomers on a support to form a random tuplet, said coupling being performed in a second reaction vessel; (3) mixing the supports from said first and second reaction vessels; and (4) dividing the mixed supports from step (3) and using the supports in step (1) and step (2), wherein steps (1) through (3) are repeated at least once; wherein said tuplets are not triplet codons.
Claim 13
Dependent Claim
15 words
The method of claim 10 further comprising step (4) cleaving said oligonucleotide from said support.
Claim 14
Dependent Claim
16 words
The method of claim 10, wherein there are four different monomers adenine, guanine, cytosine and thymine.
Claim 15
Dependent Claim
21 words
The method of claim 12, wherein four different monomers in step 1, 2, 3, or 4 are present in equal proportions.
Claim 16
Dependent Claim
18 words
The method of claim 12, wherein the four different monomers in steps 1-4 are present in equal proportions.
Claim 17
Dependent Claim
18 words
The method of claim 12, wherein the four different monomers in steps 1-4 are present in unequal proportions.
Claim 18
Dependent Claim
18 words
The method of claim 12, wherein the four different monomers in steps 1-4 are present in unequal proportions.
Claim 19
Dependent Claim
19 words
The method of claim 12, wherein the guanine and thymine monomers of step 2 are present in equal proportions.
Claim 20
Dependent Claim
19 words
The method of claim 12, wherein the guanine and thymine monomers of step 2 are present in unequal proportions.
Claim 21
Dependent Claim
21 words
The method of claim 10, wherein the mixed supports from said first and second reaction vessels are divided into equal proportions.
Claim 22
Dependent Claim
21 words
The method of claim 10, wherein the mixed supports from said first and second reaction vessels are divided into unequal proportions.
References Cited (Backward Citations)
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