Abstract The present invention provides an expression cassette encoding a plant acetyl CoA carboxylase gene and methods for conferring herbicide tolerance and/or altering the oil content of plants by introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. An expression cassette of the invention can comprise a gene coding a plant acetyl CoA carboxylase or a functional mutant thereof or an antisense DNA sequence complementary to the sequence for a plant acetyl CoA carboxylase gene combined with a promoter functional in a plant cell. The method of imparting herbicide tolerance to a plant includes the steps of introducing the expression cassette into the cells of a plant tissue and expressing the plant acetyl CoA carboxylase gene in an amount effective to render the acetyl CoA carboxylase and/or plant cell substantially tolerant to the herbicides. The method of altering the oil content in a plant includes the steps of introducing an expression cassette into plant cells and expressing the acetyl CoA carboxylase gene in an amount effective to alter the oil content of the cells. The expression cassette can also be introduced into a prokaryotic cell to increase yield of a plant acetyl CoA carboxylase so that crystallized enzyme can be used to screen and identify other herbicides that bind to and inhibit the enzyme.
An expression cassette comprising: a DNA molecule comprising DNA SEQ. ID NO:1 coding for a maize acetyl CoA carboxylase enzyme operably linked to a promoter functional in a host cell.
Claim 2
Dependent Claim
30 words
An expression cassette comprising: a DNA molecule comprising DNA SEQ. ID NO:1 coding for a maize acetyl CoA carboxylase enzyme operably linked to a promoter functional in a host cell.
Claim 3
Dependent Claim
40 words
An expression cassette according to claim 1 further comprising: a DNA molecule encoding an amino terminal maize chloroplast transit peptide, wherein the DNA molecule is operably linked between the promoter and the gene coding for the maize acetyl CoA carboxylase.
Claim 4
Dependent Claim
15 words
An expression cassette according to claim 1, wherein the promoter is a tissue specific promoter.
Claim 5
Dependent Claim
12 words
An expression cassette according to claim 1, which is on a plasmid.
Claim 6
Dependent Claim
32 words
An expression cassette according to claim 1, wherein the promoter provides for about a 2- to 20-fold increase in expression of the acetyl CoA carboxylase enzyme over that of a native enzyme.
Claim 7
Dependent Claim
27 words
An expression cassette according to claim 1, wherein the promoter is selected from the group consisting of 35S cauliflower mosaic virus promoter, and the nopaline synthase promoter.