US5487984A
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Processes for producing tumor necrosis factor
Assignee: Biogen Inc
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Filing: 1985-12-20
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Granted: 1996-01-30
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Expires: 2005-12-20
Abstract
Abstract A process of producing mammalian tumor necrosis factors (TNF) and TNF-like polypeptides by culturing eukaryotic or prokaryotic hosts transformed with DNA sequences encoding those polypeptides. A process for purifying TNF-like polypeptides using an anion exchanger. The TNFs and TNF-like polypeptides produced by the processes of this invention, and compositions and methods utilizing those TNFs and TNF-like polypeptides, are useful in anticancer, antitumor and antimalarial therapies. They are also useful together with interferon therapy, chemotherapy in anticancer and antitumor therapies, and in combination with actinomycin D in the treatment of tumor-bearing mammals.
Inventors
- 👤 Bernard Allet
- 👤 Eric H. Kawashima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 21:43
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; A; A61; A61K; A61K38/00; A61K38/16; A61K38/17; A61K38/19; A61K38/191; A61K38/21; A61P; A61P33/00; A61P33/02; A61P35/00; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/525; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
95 words
A recombinant DNA molecule characterized by a DNA sequence operatively linked to an expression control sequence selected from T4 or λpL-T4, said DNA sequence being selected from the group consisting of: (a) the DNA insert of p-mTNF-3 (DSM 3159); (b) the DNA insert of p-hTNF-1 (DSM 3160); (c) DNA sequences that hybridize to one or both of the foregoing DNA inserts and which code on expression for a tumor necrosis factor; and (d) DNA sequences that code on expression for a polypeptide coded for on expression by any of the foregoing DNA inserts and sequences.
Claim 2
Dependent Claim
95 words
A recombinant DNA molecule characterized by a DNA sequence operatively linked to an expression control sequence selected from T4 or λpL-T4, said DNA sequence being selected from the group consisting of: (a) the DNA insert of p-mTNF-3 (DSM 3159); (b) the DNA insert of p-hTNF-1 (DSM 3160); (c) DNA sequences that hybridize to one or both of the foregoing DNA inserts and which code on expression for a tumor necrosis factor; and (d) DNA sequences that code on expression for a polypeptide coded for on expression by any of the foregoing DNA inserts and sequences.
Claim 3
Dependent Claim
20 words
The recombinant DNA molecule according to claim 1, wherein the DNA sequence is selected from the group consisting of ##STR6##
Claim 4
Dependent Claim
20 words
The recombinant DNA molecule according to claim 1, wherein the DNA sequence is selected from the group consisting of ##STR7##
Claim 5
Dependent Claim
21 words
A recombinant DNA molecule selected from the group consisting of pBR322-pL-T4-hTNF (DSM 3175), pBR322-pL-T4-Δ2-hTNF, 153-pL-T4-hTNF-CA3-cts-T4ter (DSM 3460), and 153-T4-hTNF-CA5-T4ter (DSM 3461).
Claim 6
Dependent Claim
21 words
A recombinant DNA molecule selected from the group consisting of pBR322-pL-T4-hTNF (DSM 3175), pBR322-pL-T4-Δ2-hTNF, 153-pL-T4-hTNF-CA3-cts-T4ter (DSM 3460), and 153-T4-hTNF-CA5-T4ter (DSM 3461).
Claim 7
Dependent Claim
45 words
A process for producing a tumor necrosis factor polypeptide comprising the steps of culturing a prokaryotic host transformed with a recombinant DNA molecule selected from the group consisting of 153-pL-T4-hTNF-CA3(B), pBR322-pL-T4-hTNF (DSM 3175), pBR322-pL-T4-Δ2-hTNF, 153-pL-T4-hTNF-CA3-cts-T4ter (DSM 3460), and 153-T4-hTNF-CAS-T4ter (DSM 3461); and collecting said polypeptide.
Claim 8
Dependent Claim
45 words
A process for producing a tumor necrosis factor polypeptide comprising the steps of culturing a prokaryotic host transformed with a recombinant DNA molecule selected from the group consisting of 153-pL-T4-hTNF-CA3(B), pBR322-pL-T4-hTNF (DSM 3175), pBR322-pL-T4-Δ2-hTNF, 153-pL-T4-hTNF-CA3-cts-T4ter (DSM 3460), and 153-T4-hTNF-CAS-T4ter (DSM 3461); and collecting said polypeptide.
Claim 9
Dependent Claim
21 words
The process according to claim 5, wherein the host is selected from strains of E.coli, Bacillus, Streptomyces, yeasts and other fungi.
Claim 10
Dependent Claim
31 words
The process according to claim 16, wherein the transformed host is selected from the group consisting of E coli W3110 (153-pL-T4-hTNF-CA3(13), E.coli W3110 (153-pL-T4-hTNF-CA3-cts-T4ter), E.coli W3110 (153-T4-hTNF-CA5-T4ter), and E.coli WA802 (153-T4-hTNF-CA5-T4ter).
Claim 11
Dependent Claim
55 words
A process for producing a tumor necrosis factor polypeptide comprising the steps of culturing a prokaryotic host transformed with a recombinant DNA molecule characterized by a DNA sequence operatively linked to an expression control sequence selected from T4 or λpL-T4, said DNA sequence being selected from the group consisting of ##STR8## and collecting said polypeptide.
Claim 12
Dependent Claim
55 words
A process for producing a tumor necrosis factor polypeptide comprising the steps of culturing a prokaryotic host transformed with a recombinant DNA molecule characterized by a DNA sequence operatively linked to an expression control sequence selected from T4 or λpL-T4, said DNA sequence being selected from the group consisting of ##STR8## and collecting said polypeptide.
Claim 13
Dependent Claim
55 words
A process for producing a tumor necrosis factor polypeptide comprising the steps of culturing a prokaryotic host transformed with a recombinant DNA molecule characterized by a DNA sequence operatively linked to an Expression control sequence selected from T4 or λpL-T4, said DNA sequence being selected from the group consisting of ##STR9## and collecting said polypeptide.
Claim 14
Dependent Claim
55 words
A process for producing a tumor necrosis factor polypeptide comprising the steps of culturing a prokaryotic host transformed with a recombinant DNA molecule characterized by a DNA sequence operatively linked to an Expression control sequence selected from T4 or λpL-T4, said DNA sequence being selected from the group consisting of ##STR9## and collecting said polypeptide.
Claim 15
Dependent Claim
7 words
The recombinant DNA molecule 153-T4-hTNF-CA5-T4ter (DSM 3461).
Claim 16
Dependent Claim
7 words
The recombinant DNA molecule 153-T4-hTNF-CA5-T4ter (DSM 3461).
Claim 17
Dependent Claim
33 words
A process for producing a tumor necrosis factor comprising the step of culturing a prokaryotic host transformed with a recombinant DNA molecule as defined in claim 10 and collecting said tumor necrosis factor.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-01-29 | 1999-07-31 | 2000-01-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-01-29 | 2003-07-31 | 2004-01-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-01-29 | 2007-07-31 | 2008-01-30 | $7700.00 | Window Closed / Lapsed |