US4871663A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Exporession vector for human TnF
Assignee: Suntory Ltd
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Filing: 1986-09-16
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Granted: 1989-10-03
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Expires: 2006-09-16
Abstract
Abstract The present invention provides a vector plasmid capable of efficient tumor necrosis factor (TNF) production, a process capable of efficient TNF production in a host transformed with said plasmid and a composition containing the TNF produced by said process. The novel plasmid of the present invention is characterized by having inserted therein a DNA fragment that has a phage-derived promoter region upstream of a structural gene for TNF and in which a DNA fragment containing an E. coli gene-derived transcription termination coding base sequence (terminator) is joined immediately downstream of a base sequence coding for the termination of translation of said structural gene.
Inventors
- 👤 Takehiro Oshima
- 👤 Shoji Tanaka
- 👤 Shigekazu Matsukura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 21:53
CPC Classifications
C; C12; C12N; C12N15/00; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/525; C12N15/09; C12N15/63; C12N15/70; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/14; Y10S930/144; Y10S930/31
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
33 words
A plasmid capable of efficient expression of human tumor necrosis factor (TNF) in Escherichia coli (E coli), which plasmid is selected from the group consisting of pPLT4TNFST8, pT4TNFST8 and pT4TNFST8 rop - .
Claim 2
Dependent Claim
33 words
A plasmid capable of efficient expression of human tumor necrosis factor (TNF) in Escherichia coli (E coli), which plasmid is selected from the group consisting of pPLT4TNFST8, pT4TNFST8 and pT4TNFST8 rop - .
Claim 3
Dependent Claim
61 words
A process for producing human tumor necrosis factor (TNF) which comprises the steps of: (a) transforming an Escherichia coli strain with a plasmid selected from the group consisting of pPLT4TNFST8, pT4TNFST8 and pT4TNFST8 rop - ; (b) culturing the transformed Escherichia coli strain under conditions which optimize expression of TNF; and (c) extracting the purifying said TNF from the transformed strain.
Claim 4
Dependent Claim
61 words
A process for producing human tumor necrosis factor (TNF) which comprises the steps of: (a) transforming an Escherichia coli strain with a plasmid selected from the group consisting of pPLT4TNFST8, pT4TNFST8 and pT4TNFST8 rop - ; (b) culturing the transformed Escherichia coli strain under conditions which optimize expression of TNF; and (c) extracting the purifying said TNF from the transformed strain.
Claim 5
Dependent Claim
30 words
A process according to claim 2 wherein the transformed strain is selected from the group consisting of W3110/CI/pPLT4TNFST8, WA802/CI/pPLT4TNFST8, W3110/pT4TNFST8, WA802/pT4TNFST8, W3110/pT4TNFST8 rop - ; and WA802/pT4TNFST8 rop - .
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-10-02 | 1993-04-03 | 1993-10-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-10-02 | 1997-04-03 | 1997-10-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-10-02 | 2001-04-03 | 2001-10-03 | $7700.00 | Window Closed / Lapsed |