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US5457527A Expired / Lapsed US ⏱️ 0.0 years remaining

Microplate forming wells with transparent bottom walls for assays using light measurements

Assignee: Revvity Health Sciences Inc
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Filing: 1994-03-30
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Granted: 1995-10-10
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Expires: 2014-03-30
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Abstract

Abstract A microplate forms a multiplicity of sample wells for holding samples to be assayed by light emissions or light transmission. The microplate comprises a unitary upper plate and a unitary lower plate. The unitary upper plate forms the side walls of the sample wells, while the unitary lower plate forms the bottom walls of the sample wells. The side walls are opaque so that light cannot be transmitted between adjacent wells through the side walls. The bottom walls are transparent to allow the transmission of light therethrough. Bands of opaque material surround the bottom wall of each well and are located below a level of an upper surface of, the bottom wall of each well. The bands of opaque material are constructed and arranged to block the transmission of light between adjacent wells through the lower plate.

Inventors

  • 👤 Roy L. Manns
  • 👤 Alfred J. Kolb
  • 👤 Bernard S. Effertz

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-26 21:26
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/508; B01L3/5085; G; G01; G01N; G01N21/00; G01N21/01; G01N21/03; G01N21/17; G01N21/25; G01N21/251; G01N21/253; B29; B29C; B29C45/00; B29C45/16; G01N2021/0346; G01N21/62; G01N21/63; G01N21/64; G01N21/645; G01N2021/6482; G01N21/6452; G01N21/75; G01N21/76; G01N2201/00; G01N2201/06; G01N2201/064

Claims Analysis

Total Claims: 23
Claim 1
Independent Claim 133 words
A microplate forming a multiplicity of sample wells for holding samples to be assayed by light emissions or light transmission, said plate comprising a unitary upper plate forming the side walls of the sample wells, said side walls being opaque so that light cannot be transmitted between adjacent wells through said side walls, a unitary lower plate forming the bottom walls of the sample wells, said bottom walls being transparent to allow the transmission of light therethrough, said bottom walls having respective upper surfaces, and bands of opaque material surrounding the bottom wall of each well and located below a level of the upper surface of the bottom wall of each well, said bands of opaque material constructed and arranged to block the transmission of light between adjacent wells through said lower plate.
Claim 2
Dependent Claim 133 words
A microplate forming a multiplicity of sample wells for holding samples to be assayed by light emissions or light transmission, said plate comprising a unitary upper plate forming the side walls of the sample wells, said side walls being opaque so that light cannot be transmitted between adjacent wells through said side walls, a unitary lower plate forming the bottom walls of the sample wells, said bottom walls being transparent to allow the transmission of light therethrough, said bottom walls having respective upper surfaces, and bands of opaque material surrounding the bottom wall of each well and located below a level of the upper surface of the bottom wall of each well, said bands of opaque material constructed and arranged to block the transmission of light between adjacent wells through said lower plate.
Claim 3
Dependent Claim 17 words
The microplate of claim 1, wherein said bands of opaque material are disposed in said lower plate.
Claim 4
Dependent Claim 23 words
The microplate of claim 1, wherein said bands of opaque material are formed from opaque beads surrounding the bottom wall of each well.
Claim 5
Dependent Claim 19 words
The microplate of claim 3, wherein said beads are integrally formed with the lower surface of said upper plate.
Claim 6
Dependent Claim 19 words
The microplate of claim 4, further including grooves in the upper surface of said lower plate receiving said beads.
Claim 7
Dependent Claim 15 words
The microplate of claim 1, wherein said upper plate is a single molded plastic part.
Claim 8
Dependent Claim 15 words
The microplate of claim 1, wherein said lower plate is a single molded plastic part.
Claim 9
Dependent Claim 110 words
A microplate forming a multiplicity of sample wells for homing samples to be assayed by light emissions or light transmission, said plate comprising a unitary upper plate forming the side walls of the sample wells, said side walls being opaque so that light cannot be transmitted between adjacent wells through said side walls, a unitary lower plate forming the bottom walls of the sample wells, said bottom walls being transparent to allow the transmission of light therethrough, said bottom walls having respective upper surfaces, and opaque beads surrounding the bottom wall of each well and located below a level of the upper surface of the bottom wall of each well.
Claim 10
Dependent Claim 110 words
A microplate forming a multiplicity of sample wells for homing samples to be assayed by light emissions or light transmission, said plate comprising a unitary upper plate forming the side walls of the sample wells, said side walls being opaque so that light cannot be transmitted between adjacent wells through said side walls, a unitary lower plate forming the bottom walls of the sample wells, said bottom walls being transparent to allow the transmission of light therethrough, said bottom walls having respective upper surfaces, and opaque beads surrounding the bottom wall of each well and located below a level of the upper surface of the bottom wall of each well.
Claim 11
Dependent Claim 19 words
The microplate of claim 8, wherein said beads are integrally formed with the lower surface of said upper plate.
Claim 12
Dependent Claim 19 words
The microplate of claim 9, further including grooves in the upper surface of said lower plate receiving said beads.
Claim 13
Dependent Claim 33 words
The microplate of claim 8, wherein said beads form bands of opaque material surrounding the bottom wall of each well to block the transmission of light between adjacent wells through said lower plate.
Claim 14
Dependent Claim 15 words
The microplate of claim 8, wherein said upper plate is a single molded plastic part.
Claim 15
Dependent Claim 15 words
The microplate of claim 8, wherein said lower plate is a single molded plastic part.
Claim 16
Dependent Claim 116 words
A microplate forming a multiplicity of sample wells for holding samples to be assayed by light emissions or light transmission, said plate comprising a unitary upper plate forming the side walls of the sample wells, said side walls being opaque so that light cannot be transmitted between adjacent wells through said side walls, a unitary lower plate forming the bottom walls of the sample wells, said bottom walls being transparent to allow the transmission of light therethrough, and grooves formed in the upper surface of said lower plate and surrounding the bottom wall of each well, said grooves being filled with opaque material to block the transmission of light between adjacent wells through said lower plate.
Claim 17
Dependent Claim 116 words
A microplate forming a multiplicity of sample wells for holding samples to be assayed by light emissions or light transmission, said plate comprising a unitary upper plate forming the side walls of the sample wells, said side walls being opaque so that light cannot be transmitted between adjacent wells through said side walls, a unitary lower plate forming the bottom walls of the sample wells, said bottom walls being transparent to allow the transmission of light therethrough, and grooves formed in the upper surface of said lower plate and surrounding the bottom wall of each well, said grooves being filled with opaque material to block the transmission of light between adjacent wells through said lower plate.
Claim 18
Dependent Claim 15 words
The microplate of claim 14, wherein said upper plate is a single molded plastic part.
Claim 19
Dependent Claim 15 words
The microplate of claim 14, wherein said lower plate is a single molded plastic part.
Claim 20
Dependent Claim 123 words
A microplate forming a multiplicity of sample wells for holding samples to be assayed by light emissions or light transmission, said plate comprising a unitary upper plate forming the side walls of the sample wells, said side walls being opaque so that light cannot be transmitted between adjacent wells through said side walls, a unitary lower plate forming the bottom walls of the sample wells, said bottom walls being transparent to allow the transmission of light therethrough, and opaque material disposed at a surface of said lower plate between adjacent wells and beneath a level of an upper surface of the bottom wall of each sample well so as to reduce the transmission of light between the adjacent wells through said lower plate.
Claim 21
Dependent Claim 123 words
A microplate forming a multiplicity of sample wells for holding samples to be assayed by light emissions or light transmission, said plate comprising a unitary upper plate forming the side walls of the sample wells, said side walls being opaque so that light cannot be transmitted between adjacent wells through said side walls, a unitary lower plate forming the bottom walls of the sample wells, said bottom walls being transparent to allow the transmission of light therethrough, and opaque material disposed at a surface of said lower plate between adjacent wells and beneath a level of an upper surface of the bottom wall of each sample well so as to reduce the transmission of light between the adjacent wells through said lower plate.
Claim 22
Dependent Claim 11 words
The microplate of claim 17, wherein said opaque material is dark-colored.
Claim 23
Dependent Claim 11 words
The microplate of claim 18, wherein said opaque material is black.

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1998-10-09 1999-04-10 1999-10-10 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 2002-10-09 2003-04-10 2003-10-10 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2006-10-09 2007-04-10 2007-10-10 $7700.00 Window Closed / Lapsed

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