US4741619A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Hydrophilic microplates for vertical beam photometry
Assignee: MOECULAR DEVICES Corp A CORP OF
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Filing: 1987-05-05
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Granted: 1988-05-03
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Expires: 2007-05-05
Abstract
Abstract A microtiter plate prepared from a hydrophobic plastic is rendered more hydrophilic by treatment of the surfaces of the wells of the microplate prior to use. The resulting plates are particularly useful in vertical beam photometry.
Inventors
- 👤 Gillian M. K. Humphries
- 👤 Viola T. Kung
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 22:33
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/508; B01L3/5085; B29; B29C; B29C59/00; B29C59/14; G; G01; G01N; G01N21/00; G01N21/01; G01N21/03; G01N2201/00; G01N2201/04; G01N2201/0446
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
61 words
A method for improving the precision of optical density measurements made utilizing a vertical beam photometer to determine the presence or amount of analyte in the wells of a microplate, which comprises: treating the surfaces of the wells of said microplate in order to increase the hydrophilic character of said surfaces prior to adding aqueous sample or reagent to said wells.
Claim 2
Independent Claim
61 words
A method for improving the precision of optical density measurements made utilizing a vertical beam photometer to determine the presence or amount of analyte in the wells of a microplate, which comprises: treating the surfaces of the wells of said microplate in order to increase the hydrophilic character of said surfaces prior to adding aqueous sample or reagent to said wells.
Claim 3
Dependent Claim
14 words
The method of claim 1, wherein treating comprises coating said surfaces with a surfactant.
Claim 4
Dependent Claim
25 words
The method of claim 2, wherein said surfactant is selected from the group consisting of polyoxyethylene sorbitan fatty acid esters and polyoxyethylene fatty acid esters.
Claim 5
Dependent Claim
24 words
The method of claim 2, wherein said surfactant is applied to said surface at the rate of from 0.01 to 1 μg/cm 2 .
Claim 6
Dependent Claim
17 words
The method of claim 1, wherein treating said surfaces comprises subjecting said surfaces to an electric discharge.
Claim 7
Dependent Claim
16 words
The method of claim 1, wherein treating said surfaces comprises covalent attachment of hydrophilic chemical moieties.
Claim 8
Dependent Claim
16 words
The method of claim 1, wherein said treating occurs during the process of manufacturing said microplate.
Claim 9
Dependent Claim
36 words
A microplate for use in vertical beam photometry, wherein the surfaces of the wells of said microplate comprise a hydrophobic plastic treated to increase hydrophilicity beyond that which is present for said hydrophobic plastic when untreated.
Claim 10
Dependent Claim
36 words
A microplate for use in vertical beam photometry, wherein the surfaces of the wells of said microplate comprise a hydrophobic plastic treated to increase hydrophilicity beyond that which is present for said hydrophobic plastic when untreated.
Claim 11
Dependent Claim
16 words
The microplate of claim 8, wherein said plastic comprises polystyrene or a copolymer containing styrene monomers.
Claim 12
Dependent Claim
13 words
The microplate of claim 9, wherein said polystyrene is coated with a surfactant.
Claim 13
Dependent Claim
25 words
The microplate of claim 10, wherein said surfactant is selected from the group consisting of polyoxyethylene fatty acid esters and polyoxyethylene sorbitan fatty acid esters.
Claim 14
Dependent Claim
17 words
The microplate of claim 8, wherein said surfaces have been subjected to corona discharge or plasma etching.
Claim 15
Dependent Claim
17 words
The microplate of claim 8, wherein said surfaces are modified by covalent attachment of hydrophilic chemical moieties.
Claim 16
Dependent Claim
19 words
The microplate of claim 8, wherein the wetting angle for water on said treated surfaces is less than 3°.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-05-03 | 1991-11-02 | 1992-05-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-05-03 | 1995-11-02 | 1996-05-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-05-03 | 1999-11-02 | 2000-05-03 | $7700.00 | Window Closed / Lapsed |