Method for inducing periodontal tissue regeneration

Claim 1

A method for inducing periodontal regeneration in a wound of a mammal with periodontal disease or other condition requiring periodontal regeneration, said method induces soft tissue, cementum and alveolar bone healing of a type characteristic of the anatomy and architecture of undiseased periodontium, comprising the steps of: a) treating the root surface in the wound with mechanical instrumentation and a composition comprising a demineralizing agent; b) applying a therapeutically effective amount of a growth factor directly to the treated root surface, wherein said growth factor is one or more factors selected from the group consisting of platelet-derived growth factor in a form having two beta chain (PDGF-BB), platelet-derived growth factor in a form having an alpha and a beta chain (PDGF-AB) and IGF-I; c) inserting a periodontal barrier, wherein the barrier is positioned between the gingival tissue and the root surface treated according to steps. a) and b) to create and maintain a space for regeneration, and wherein the barrier is selected the group consisting of a membrane, a biodegradable polymer, and a biocompatible porous material; and d) closing the wound to allow for regeneration.

Claim 2

A method for inducing periodontal regeneration in a wound of a mammal with periodontal disease or other condition requiring periodontal regeneration, said method induces soft tissue, cementum and alveolar bone healing of a type characteristic of the anatomy and architecture of undiseased periodontium, comprising the steps of: a) treating the root surface in the wound with mechanical instrumentation and a composition comprising a demineralizing agent; b) applying a therapeutically effective amount of a growth factor directly to the treated root surface, wherein said growth factor is one or more factors selected from the group consisting of platelet-derived growth factor in a form having two beta chain (PDGF-BB), platelet-derived growth factor in a form having an alpha and a beta chain (PDGF-AB) and IGF-I; c) inserting a periodontal barrier, wherein the barrier is positioned between the gingival tissue and the root surface treated according to steps. a) and b) to create and maintain a space for regeneration, and wherein the barrier is selected the group consisting of a membrane, a biodegradable polymer, and a biocompatible porous material; and d) closing the wound to allow for regeneration.

Claim 8

A method for inducing regeneration in a wound site comprising damaged bone, periodontium, connective tissue, or ligament of a mammal, said method induces soft tissue and bone regeneration resulting in soft tissue, cementum and bone characteristic of the anatomy and architecture of undamaged tissue in the regeneration site of the mammal, comprising the steps of: a) treating damaged root surface in the wound site with mechanical instrumentation and a composition comprising a demineralizing agent; b) applying a therapeutically effective amount of a growth factor directly to the treated root surface, wherein said growth factor is one or more factors selected from the group consisting of platelet-derived growth factor in a form having two beta chain (PDGF-BB), platelet-derived growth factor in a form having an alpha and a beta chain (PDGF-AB) and IGF-I; c) inserting a barrier, wherein the barrier is positioned between the surrounding tissue of the wound site and the root surface treated according to steps a) and b) to create and maintain a space for regeneration, and wherein the barrier is selected the group consisting of a membrane, a biodegradable polymer, and a biocompatible porous material; and d) closing the wound to allow for regeneration.

Claim 9

A method for inducing regeneration in a wound site comprising damaged bone, periodontium, connective tissue, or ligament of a mammal, said method induces soft tissue and bone regeneration resulting in soft tissue, cementum and bone characteristic of the anatomy and architecture of undamaged tissue in the regeneration site of the mammal, comprising the steps of: a) treating damaged root surface in the wound site with mechanical instrumentation and a composition comprising a demineralizing agent; b) applying a therapeutically effective amount of a growth factor directly to the treated root surface, wherein said growth factor is one or more factors selected from the group consisting of platelet-derived growth factor in a form having two beta chain (PDGF-BB), platelet-derived growth factor in a form having an alpha and a beta chain (PDGF-AB) and IGF-I; c) inserting a barrier, wherein the barrier is positioned between the surrounding tissue of the wound site and the root surface treated according to steps a) and b) to create and maintain a space for regeneration, and wherein the barrier is selected the group consisting of a membrane, a biodegradable polymer, and a biocompatible porous material; and d) closing the wound to allow for regeneration.