US5436151A
Expired / Lapsed
US
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Method for culturing human hematopoietic stem cells in vitro
Assignee: University of Minnesota System
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Filing: 1992-04-03
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Granted: 1995-07-25
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Expires: 2012-04-03
Abstract
Abstract A method for culturing mammalian hematopoietic stem cells is provided comprising maintaining a population of human stem cells in fixed, non-contacting relationship to a population of cultured stromal cells, which populations are in liquid stromal growth medium connection, so that the ability of the stem cells to differentiate and self-replicate is maintained during an extended culture period.
Inventors
- 👤 Philip B. McGlave
- 👤 Catherine M. Verfaillie
- 👤 Jeffrey S. Miller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 04:36
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0634; C12N5/0647; C12N2501/00; C12N2501/20; C12N2501/21; C12N2501/23; C12N2501/30; C12N2501/38; C12N2501/39; C12N2502/00; C12N2502/13; C12N2502/1394
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
75 words
A cell culture method comprising culturing in vitro a population of hematopoietic human stem cells supported in a non-contacting relationship to a supported population of cultured stromal cells, which populations are in a body of liquid stromal cell growth medium wherein the growth medium does not contain exogenous cytokines, so that the ability of the stem cell population to self-replicate and to differentiate into granulocyte-macrophage progenitors and long-term bone marrow culture initiating cells is maintained.
Claim 2
Dependent Claim
75 words
A cell culture method comprising culturing in vitro a population of hematopoietic human stem cells supported in a non-contacting relationship to a supported population of cultured stromal cells, which populations are in a body of liquid stromal cell growth medium wherein the growth medium does not contain exogenous cytokines, so that the ability of the stem cell population to self-replicate and to differentiate into granulocyte-macrophage progenitors and long-term bone marrow culture initiating cells is maintained.
Claim 3
Dependent Claim
18 words
The method of claim 1 wherein the stem cells are derived from a population of bone marrow cells.
Claim 4
Dependent Claim
17 words
The method of claim 2 wherein the stem cells are Lin - CD34 + DR - .
Claim 5
Dependent Claim
13 words
The method of claim 1 wherein the stromal cells are human stromal cells.
Claim 6
Dependent Claim
16 words
The method of claim 1 wherein the stromal cell population is supported on a microporous membrane.
Claim 7
Dependent Claim
22 words
The method of claim 5 wherein the stromal cell population and the stem cell population are cultured on separable cell culture substrata.
Claim 8
Dependent Claim
28 words
The method of claim 6 wherein the stem cell population is supported by a microporous membrane, which membrane separates the stromal cell population from the stem cell population.
Claim 9
Dependent Claim
20 words
The method of claim 6 wherein the stromal population is adhered to the bottom of a plastic cell culture well.
Claim 10
Dependent Claim
21 words
The method of claim 1 wherein the stem cell population and the stromal cell population are separated by about 0.25-2 mm.
Claim 11
Independent Claim
24 words
The method of claims 1 or 8 wherein the liquid stromal cell growth medium is 25-100% exchanged at intervals of about 8 hrs-14 days.
Claim 12
Dependent Claim
22 words
The method of claim 8 wherein the liquid stromal cell growth medium is continuously circulated through a chamber comprising said stromal cells.
Claim 13
Dependent Claim
10 words
The method of claim 4 wherein the populations are allogeneic.
Claim 14
Dependent Claim
10 words
The method of claim 4 wherein the populations are autologous.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-07-24 | 1999-01-23 | 1999-07-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-07-24 | 2003-01-23 | 2003-07-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-07-24 | 2007-01-23 | 2007-07-25 | $7700.00 | Window Closed / Lapsed |