Biocompatible, low protein adsorption affinity matrix

Claim 1

An affinity matrix for chromatography and immobilization of biologically active materials comprising a support having a polyurethane coating thereon of said polymer thereon, wherein said polyurethane polymer is derived from prepolymer units of oxyethylene based alcohols having essentially all of the hydroxyl groups capped with polyisocyanate groups and having at least 15% of said polyisocyanate groups reacted with a modifying compound having a first NCO reactive group and a second functional group having a substantially less NCO reactive group, and wherein said polyurethane polymer has been activated by converting said second functional groups to an active functional group capable of covalently attaching a bioaffinity agent, said matrix being characterized by a biocompatible surface which resists nonspecific protein adsorption, wherein the modifying compound is selected from the group consisting of ethanolamine; aminoethyl hydrogensulfate; taurine; 4-aminosulfonyl-1-hydroxy-2-napththoic acid; glucosamine; 5-(aminosulfonyl) N-((1-ethyl-2-pyrrolidinyl)methyl)-2-methoxybenzamide; sulfamylphenyl-D-glucosylamine; 4-carboxybenzene-sulfonamide; sulfanilamide; cyclic-adenosine monophosphate; 2-aminoethyl phosphonic acid; tyrosine; tyramine; dibutylamine; L- or DL-cysteine; L- or DL-cysteine ethyl ester; L- or DL-cystine dimethyl ester; L- or DL-Cystine; L- or DL-cysteinesulfonic acid; L- or DL-cysteic acid; cystamine; 2-mercaptoethanol; ethanethiol; glutathione; 3-amino-1,2-propanediol; 3-amino-1-propane sulfonic acid; 3-aminophenyl boronic acid; 2-amino-2-deoxy-D-galactose; 1-amino-1-deoxy-D-galactose; p-aminophenyl-alpha-D-glucose; p-aminophenyl-1-thio-beta-D-galactose; penicillamine; peptides with sulfhydryl groups; peptides with free amino groups; animal hormones; polysaccharides; lipids; nucleic acids; amino sugars; amino acids; amine surfactants; diamines and polyamines.