US4412000A
Expired / Lapsed
US
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Method of binding a biologically active material to a carrier containing hydroxyl groups
Assignee: BIOCOMPAT Inc A CORP OF WA
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Filing: 1981-11-12
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Granted: 1983-10-25
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Expires: 2001-11-12
Abstract
Abstract Biologically active material such as an enzyme is bonded to a carrier containing hydroxyl groups by binding an isocyanate compound to the carrier and bonding the biologically active material to the bound isocyanate compound. Binding of the isocyanate compound to the carrier is achieved with the use of a non-toxic titanium based compound which catalyzes the formation of urethane bonds. The preferred non-toxic based titanium compound is an orthotitanium acid ester such as tetrabutyltitanate.
Inventors
- 👤 Hans-Dieter Lehmann
- 👤 Gerd G. Krisam
- 👤 Ruth S. Golla
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 02:20
CPC Classifications
C; C08; C08G; C08G18/00; C08G18/06; C08G18/28; C08G18/40; C08G18/64; C08G18/6415; C08G18/6446; C12; C12N; C12N11/00; C12N11/02
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
61 words
A method of binding a biologically active material to a carrier containing hydroxyl groups, said method comprising the steps of reacting a sufficient amount of an isocyanate compound with said carrier in the presence of an orthotitanium acid ester which catalyzes the formation of urethane bonds to form an isocyanate-containing carrier; and binding said biologically active material to the isocyanate-containing carrier.
Claim 2
Independent Claim
61 words
A method of binding a biologically active material to a carrier containing hydroxyl groups, said method comprising the steps of reacting a sufficient amount of an isocyanate compound with said carrier in the presence of an orthotitanium acid ester which catalyzes the formation of urethane bonds to form an isocyanate-containing carrier; and binding said biologically active material to the isocyanate-containing carrier.
Claim 3
Independent Claim
72 words
A method of binding a biologically active material to a carrier containing hydroxyl groups, said method comprising the steps of reacting a sufficient amount of an isocyanate compound with said carrier in the presence of a non-toxic titanium based compound which catalyzes the formation of urethane bonds to form an isocyanate-containing carrier; treating the isocyanate-containing carrier to remove therefrom titanium based compound; and binding said biologically active material to the isocyanate-containing carrier.
Claim 4
Independent Claim
72 words
A method of binding a biologically active material to a carrier containing hydroxyl groups, said method comprising the steps of reacting a sufficient amount of an isocyanate compound with said carrier in the presence of a non-toxic titanium based compound which catalyzes the formation of urethane bonds to form an isocyanate-containing carrier; treating the isocyanate-containing carrier to remove therefrom titanium based compound; and binding said biologically active material to the isocyanate-containing carrier.
Claim 5
Dependent Claim
23 words
A method according to claim 2, wherein said non-toxic titanium based compound catalyzing the formation of urethane bonds is an orthotitanium acid ester.
Claim 6
Dependent Claim
22 words
A method according to claim 1 or 2, wherein said orthotitanium acid ester is an ester containing low molecular weight alkyl groups.
Claim 7
Dependent Claim
26 words
A method according to claim 4, wherein said low molecular weight alkyl groups are selected from methyl, ethyl, n-propyl, i-propyl, n-butyl and isomers and mixtures thereof.
Claim 8
Dependent Claim
15 words
A method according to claim 1 or 2, wherein said urethane forming catalyst is tetrabutyltitanate.
Claim 9
Dependent Claim
22 words
A method according to claim 1 or 2 wherein said isocyanate compound is selected from diisocyanates, triisocyanates, polyisocyanates and prepolymers formed thereof.
Claim 10
Dependent Claim
24 words
A method according to claim 7, wherein said isocyanate compound is a triisocyanate, said carrier is cellulose, and said biologically active material is urease.
Claim 11
Dependent Claim
19 words
A method according to claim 8, wherein said isocyanate compound is selected from hexamethylene diisocyanate, trimethylhexamethylene diisocyanate and N,N',N"-tris(6-isocyanatohexyl)biuret.
Claim 12
Dependent Claim
22 words
A method according to claim 8, wherein said isocyanate compound is dissolved in a solvent selected from dichloromethane, ether, acetone and frigen.
Claim 13
Dependent Claim
25 words
A method according to claim 10, wherein said isocyanate solution contains about 1 to about 24 g isocyanate compound per 100 ml of said solution.
Claim 14
Dependent Claim
40 words
A method according to claim 7, wherein said non-toxic titanium based compound catalyzing the formation of urethane bonds is in the form of a solution of an orthotitanium acid ester in a solvent selected from ether, dichloromethane, acetone and frigen.
Claim 15
Dependent Claim
28 words
A method according to claim 12, wherein said orthotitanium acid ester solution is a solution containing between about 0.3 and about 1.5 g tetrabutyltitanate per 100 ml dichloromethane.
References Cited (Backward Citations)
US3841969A
Cited
US4177038A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-10-24 | 1987-04-25 | 1987-10-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-10-24 | 1991-04-25 | 1991-10-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-10-24 | 1995-04-25 | 1995-10-25 | $7700.00 | Window Closed / Lapsed |