US5393670A
Expired / Lapsed
US
⏱️ 0.0 years remaining
DNA, vectors and transformed hosts encoding Trichoderma reesei endoglucanase I
Assignee: AB Enzymes Oy
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Filing: 1993-07-23
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Granted: 1995-02-28
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Expires: 2013-07-23
Abstract
Abstract Methods and means for the construction of strains of yeast capable of producing cellulolytic enzymes, achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expression of these cellulolytic genes in yeast leads to the production of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze 3-1,4-glucan substrates such as cellulose.
Inventors
- 👤 Jonathan Knowles
- 👤 Merja Penttila
- 👤 Tuula Teeri
- 👤 Helena M. K. Nevalainen
- 👤 Paivi Lehtovaara-Helenius
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 09:54
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2437; C12N15/00; C12N15/09; C12N15/11; C12N15/62; C12N15/625; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C12Y; C12Y302/00; C12Y302/01; C12Y302/01091; C07; C07K; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/034; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/945
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
26 words
A substantially pure DNA molecule comprising DNA encoding a polypeptide comprising the mature endoglucanase I protein amino acid sequence shown in FIG. 6 or FIG. 11.
Claim 2
Dependent Claim
26 words
A substantially pure DNA molecule comprising DNA encoding a polypeptide comprising the mature endoglucanase I protein amino acid sequence shown in FIG. 6 or FIG. 11.
Claim 3
Dependent Claim
44 words
The DNA molecule of claim 1, additionally comprising DNA encoding the endoglucanase I signal sequence, said DNA encoding said signal sequence being operably linked to said DNA encoding said polypeptide, said DNA for said signal sequence having the DNA sequence encoding the peptide MetAlaProSerValThrLeuProLeuThrThrAlaIleLeuAlaIleAlaArgLeuValAlaAla.
Claim 4
Dependent Claim
20 words
The DNA molecule of claim 2, wherein said DNA encoding said endoglucanase I signal sequence has the DNA sequence ATGGCGCCCTCAGTTACACTGCCGTTGACCACGGCCATCCTGGCNATTGCCCGGCTCGTCGCCGCC.
Claim 5
Dependent Claim
35 words
A substantially pure DNA molecule comprising the cDNA sequence shown in FIG. 6 or FIG. 11, encoding a polypeptide comprising the mature endoglucanase I protein amino acid sequence shown in FIG. 6 or FIG. 11.
Claim 6
Dependent Claim
35 words
A substantially pure DNA molecule comprising the cDNA sequence shown in FIG. 6 or FIG. 11, encoding a polypeptide comprising the mature endoglucanase I protein amino acid sequence shown in FIG. 6 or FIG. 11.
Claim 7
Dependent Claim
44 words
The DNA molecule of claim 4, additionally comprising DNA encoding the endoglucanase I signal sequence, said DNA encoding said signal sequence being operably linked to said DNA encoding said polypeptide, said DNA for said signal sequence having the DNA sequence encoding the peptide MetAlaProSerValThrLeuProLeuThrThrAlaIleLeuAlaIleAlaArgLeuValAlaAla.
Claim 8
Dependent Claim
20 words
The DNA molecule of claim 5, wherein said DNA encoding said endoglucanase I signal sequence has the DNA sequence ATGGCGCCCTCAGTTACACTGCCGTTGACCACGGCCATCCTGGCNATTGCCCGGCTCGTCGCCGCC.
Claim 9
Dependent Claim
33 words
A substantially pure DNA molecule encoding an amino acid sequence comprising the endoglucanase I signal sequence shown in FIG. 6 or FIG. 11, said endoglucanase I signal DNA sequence encoding the peptide MetAlaProSerValThrLeuProLeuThrThrAlaIleLeuAlaIleAlaArgLeuValAlaAla.
Claim 10
Dependent Claim
33 words
A substantially pure DNA molecule encoding an amino acid sequence comprising the endoglucanase I signal sequence shown in FIG. 6 or FIG. 11, said endoglucanase I signal DNA sequence encoding the peptide MetAlaProSerValThrLeuProLeuThrThrAlaIleLeuAlaIleAlaArgLeuValAlaAla.
Claim 11
Dependent Claim
21 words
The substantially pure DNA molecule of claim 7, wherein said DNA sequence encoding signal DNA sequence has the DNA sequence ATGGCGCCCTCAGTTACACTGCCGTTGACCACGGCCATCCTGGCNATTGCCCGGCTCGTCGCCGCC.
Claim 12
Dependent Claim
21 words
A recombinant DNA vector comprising the DNA sequence of any of claims 1, 3, 4, 6, 8, 2, 5, or 7.
Claim 13
Dependent Claim
10 words
A host cell transformed with the vector of claim 9.
Claim 14
Dependent Claim
13 words
The host cell of claim 10 wherein said host cell is E. coli.
Claim 15
Dependent Claim
13 words
The host cell of claim 10 wherein said host is a yeast host.
Claim 16
Dependent Claim
15 words
The yeast host cell of claim 12, wherein said host is of the genus Saccharomyces.
Claim 17
Dependent Claim
14 words
The yeast host cell of claim 13, wherein said Saccharomyces host is S. cerevisiae.
Claim 18
Dependent Claim
2 words
Plasmid pTT11.
Claim 19
Dependent Claim
2 words
Plasmid pTT11.
Claim 20
Dependent Claim
8 words
E. coli host ALKO2296 having deposit number DSM6873.
Claim 21
Dependent Claim
8 words
E. coli host ALKO2296 having deposit number DSM6873.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-02-27 | 1998-08-29 | 1999-02-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-02-27 | 2002-08-29 | 2003-02-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-02-27 | 2006-08-29 | 2007-02-28 | $7700.00 | Window Closed / Lapsed |