EP0137280A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Recombinant fungal cellobiohydrolases
Assignee: AB Enzymes Oy
•
Filing: 1984-08-29
•
Granted: —
•
Expires: 2004-08-29
Abstract
Abstract Methods and materials for obtaining recombinant forms of fungal cellulase enzymes are disclosed. An uninterrupted coding sequence for a desired cellulase can be obtained by manipulation of the genomic DNA of a cellulolytic fungus, and ligated into appropriate vectors for expression as mature, glycosylated, functional active proteins in yeast.
Inventors
- 👤 Sharon Payne Shoemaker
- 👤 David Harrow Gelfand
- 👤 Michael Alan Innis
- 👤 Shirley Yee Kwok
- 👤 Martha Baillie Ladner
- 👤 Vicky Schweickart
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 17:04
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2437; C12N15/00; C12N15/09; C12N15/10; C12N15/11; C12N15/62; C12N15/625; C12N15/63; C12N15/70; C12N15/79; C12N15/80; C12N15/81; C12N9/2445; C12Y; C12Y302/00; C12Y302/01; C12Y302/01004; C12Y302/01021; C12Y302/01091; C07; C07K; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/036; C07K2319/90; C07K2319/91
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
28 words
A recombinant DNA sequence which encodes, without interruption, an amino acid sequence substantially equivalent to the amino acid sequence of a cellulase derived from a species of fungus.
Claim 2
Dependent Claim
28 words
A recombinant DNA sequence which encodes, without interruption, an amino acid sequence substantially equivalent to the amino acid sequence of a cellulase derived from a species of fungus.
Claim 3
Dependent Claim
16 words
The DNA sequence of Claim 1, wherein the species of fungus is a species of. Trichoderma.
Claim 4
Dependent Claim
14 words
The DNA sequence of Claim 2, wherein the species of fungus is T. reesei.
Claim 5
Dependent Claim
12 words
The DNA sequence of Claim 1, wherein the cellulase is a cellobiohydrolase.
Claim 6
Dependent Claim
13 words
The DNA sequence of Claim 4, wherein the cellobiohydrolase is T. reesei cellobiohydrolase-I.
Claim 7
Dependent Claim
12 words
The DNA sequence of Claim 1, wherein the cellulase is an endoglucanase.
Claim 8
Dependent Claim
13 words
The DNA sequence of Claim 6, wherein the endoglucanase is T. reesei endoglucanase-I.
Claim 9
Dependent Claim
13 words
The DNA sequence of Claim 1, wherein the cellulase is T. reesei p-glucosidase.
Claim 10
Dependent Claim
21 words
A mature, glycosylated, functionally active fungal cellulase produced by yeast transformed with a yeast expression vector containing a fungal-derived cellulase gene.
Claim 11
Dependent Claim
21 words
A mature, glycosylated, functionally active fungal cellulase produced by yeast transformed with a yeast expression vector containing a fungal-derived cellulase gene.
Claim 12
Dependent Claim
17 words
The cellulase of Claim 9, which is substantially equivalent, in amino acid sequence, to a Trichodermal cellobiohydrolase.
Claim 13
Dependent Claim
18 words
The cellulase of Claim 10, which is substantially equivalent, in amino acid sequence, to cellobiohydrolase-I from T. reesei.
Claim 14
Dependent Claim
17 words
The endoglucanase of Claim 7, which is substantially equivalent, in amino acid sequence, to a Trichodermal endoglucanase.
Claim 15
Dependent Claim
18 words
The cellobiohydrolase of Claim 12, which is substantially equivalent, in amino acid sequence, to endoglucanase-I from T. reesei.
Claim 16
Independent Claim
43 words
A method of treating cellulose comprising: providing an expression vector capable of transforming yeast to express a mature, glycosylated, functionally active fungal cellulase, transforming yeast with such expression vector to produce such cellulase, and reacting the cellulose enzymatically with the cellulase so produced.
Claim 17
Independent Claim
43 words
A method of treating cellulose comprising: providing an expression vector capable of transforming yeast to express a mature, glycosylated, functionally active fungal cellulase, transforming yeast with such expression vector to produce such cellulase, and reacting the cellulose enzymatically with the cellulase so produced.
Claim 18
Dependent Claim
15 words
The method of Claim 10, wherein the transformed yeast are capable of secreting the cellulase.
Claim 19
Dependent Claim
22 words
The method of Claim 15, wherein the cellulase produced is encoded by an uninterrupted coding sequence derived from a Trichodermal endoglucanase gene.
Claim 20
Dependent Claim
23 words
The method of Claim 16, wherein the cellulase produced is encoded by an uninterrupted coding sequence derived from a T. reesei endoglucanase-I gene.
Claim 21
Dependent Claim
22 words
The method of Claim 15, wherein the cellulase produced is encoded by an uninterrupted coding sequence derived from a Trichodermal cellobiohydrolase gene.
Claim 22
Dependent Claim
23 words
The method of Claim 18, wherein the cellulase produced is encoded by an uninterrupted coding sequence derived from a T. reesei cellobiohydrolase-I gene.
Claim 23
Dependent Claim
17 words
The method of Claim 15, wherein said reacting is carried out in the presence of such yeast.
Claim 24
Dependent Claim
18 words
The method of Claim 15, which further includes separating such cellulase from the yeast prior to said reacting.
Claim 25
Dependent Claim
25 words
The method of Claim 15, wherein such cellulase is a cellobiohydrolase and said reacting further includes reacting the cellulose with a p-glucosidase and/or an endoglucanase.
Claim 26
Dependent Claim
25 words
The method of Claim 15, wherein such cellulase is an endoglucanase and said reacting further includes reacting the cellulose with a cellobiohydrolase and/or a p-glucosidase.
Claim 27
Dependent Claim
46 words
An expression vector for expressing a mature heterologous protein in yeast comprising, in a 5'- to -3' direction, a yeast promoter sequence, a cellulase signal sequence operatively linked to the promoter sequences, and the coding region of the heterologous protein operatively linked to the signal sequence.
Claim 28
Dependent Claim
46 words
An expression vector for expressing a mature heterologous protein in yeast comprising, in a 5'- to -3' direction, a yeast promoter sequence, a cellulase signal sequence operatively linked to the promoter sequences, and the coding region of the heterologous protein operatively linked to the signal sequence.
Claim 29
Dependent Claim
22 words
The vector of Claim 24, which further includes a yeast terminator sequence operatively joined to the 3' end of said coding region.
Claim 30
Dependent Claim
14 words
The vector of Claim 24, wherein said promoter sequence includes a yeast enolase-1 promoter.
Claim 31
Dependent Claim
21 words
The vector of Claim 24, wherein the signal sequence includes the leader coding sequence derived from the T. reesei endoglucanase-I gene.
Claim 32
Dependent Claim
21 words
The vector of Claim 24, wherein the signal sequence includes the leader coding sequence derived from the T. reesei cellobiohydrolase-I gene.
Claim 33
Dependent Claim
19 words
The vector of Claim 24, wherein the coding region includes an uninterrupted coding sequence which encodes a fungal cellulase.
Claim 34
Dependent Claim
13 words
The vector of Claim 29, wherein the fungal cellulase is T. reesei endoglucanase-I.
Claim 35
Dependent Claim
13 words
The vector of Claim 29, wherein the fungal cellulase is T. reesei cellobiohydrolase-I.
References Cited (Backward Citations)
EP0011767A1
Cited
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.