EP0088632A2
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EP
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Expression, processing and secretion of heterologous protein by yeast
Assignee: Genentech Inc
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Filing: 1983-03-08
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Granted: —
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Expires: 2003-03-08
Abstract
Abstract A yeast host organism is genetically altered via recombinant DNA technology so as to be directed to express, process and secrete a polypeptide ordinarily heterologous to the host which is recoverable in discrete form, free from unwanted polypeptide presequences or other artifact of expression.
Inventors
- 👤 Ronald Arthur Hitzeman
- 👤 David Wai-Hung Leung
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 18:17
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/575; C07K14/61; C07K2319/00; C07K2319/01; C07K2319/02; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/12; Y10S930/14; Y10S930/142; Y10S930/30
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
33 words
A product of expression, processing and secretion by a yeast organism, which is a protein heterologous to the yeast organism, in discrete form unaccompanied by unwanted polypeptide presequence or other artifact of expression.
Claim 2
Dependent Claim
33 words
A product of expression, processing and secretion by a yeast organism, which is a protein heterologous to the yeast organism, in discrete form unaccompanied by unwanted polypeptide presequence or other artifact of expression.
Claim 3
Dependent Claim
11 words
A product according to claim 1 which is a human interferon.
Claim 4
Dependent Claim
12 words
A product according to claim 2 which is a human leukocyte interferon.
Claim 5
Dependent Claim
14 words
A product according to claim 3 which is human leukocyte interferon A or D.
Claim 6
Dependent Claim
62 words
A yeast organism cell culture capable of producing a protein heterologous to the yeast organism comprising 1) viable yeast cells transformed with an expression vehicle functionally harboring DNA encoding said protein together with a heterologous signal polypeptide therefor and 2) a medium supporting the cell culture, the medium containing said protein as a product of the yeast organism expression, processing and secretion.
Claim 7
Dependent Claim
62 words
A yeast organism cell culture capable of producing a protein heterologous to the yeast organism comprising 1) viable yeast cells transformed with an expression vehicle functionally harboring DNA encoding said protein together with a heterologous signal polypeptide therefor and 2) a medium supporting the cell culture, the medium containing said protein as a product of the yeast organism expression, processing and secretion.
Claim 8
Dependent Claim
16 words
A cell culture according to claim 5 wherein the yeast organism is Saccharom y ces cerevisiae.
Claim 9
Dependent Claim
31 words
A cell culture according to claim 5 or 6 wherein the heterologous signal polypeptide is is a hybrid of signal native to said protein and of a second heterologous signal polypeptide.
Claim 10
Dependent Claim
44 words
A medium of a yeast organism cell culture as claimed in claim 5, 6 or 7 substantially free from viable or disrupted yeast cells and containing a protein heterologous to the yeast organism as a product of the yeast organism expression, processing and secretion.
Claim 11
Dependent Claim
17 words
A yeast organism cell culture capable of exporting into a medium therefor heterologous protein in discrete form.
Claim 12
Dependent Claim
17 words
A yeast organism cell culture capable of exporting into a medium therefor heterologous protein in discrete form.
Claim 13
Dependent Claim
16 words
A cell culture according to claim 9 wherein the yeast organism is Saccharom y ces cerevisiae.
Claim 14
Dependent Claim
16 words
Yeast expression vehicle YEplPT which functionally harbors DNA encoding a protein heterologous to the yeast organism.
Claim 15
Dependent Claim
16 words
Yeast expression vehicle YEplPT which functionally harbors DNA encoding a protein heterologous to the yeast organism.
Claim 16
Dependent Claim
25 words
A vehicle according to claim 11 which additionally harbors DNA encoding a heterologous signal polypeptide in translational reading franewith the DNA encoding said heterologous protein.
Claim 17
Dependent Claim
28 words
A vehicle according to claim 12 wherein the heterologous signal polypeptide is a hybrid of signal native to said heterologous protein and of a second heterologous signal polypeptide.
Claim 18
Dependent Claim
23 words
A yeast expression vehicle which functionally harbors DNA encoding human leukocyte DA signal polypeptide in translational reading frame with human leukocyte A protein.
Claim 19
Dependent Claim
23 words
A yeast expression vehicle which functionally harbors DNA encoding human leukocyte DA signal polypeptide in translational reading frame with human leukocyte A protein.
Claim 20
Dependent Claim
61 words
A process for obtaining protein heterologous to a yeast organism as a product of yeast expression, processing and secretion which process comprises transforming a yeast organism with an expression vehicle funtionally harboring DNA encoding said protein and a heterologous signal polypeptide, preparing a culture of the transformed organism, growing the culture and recovering said prtein from the medium of the culture.
Claim 21
Dependent Claim
61 words
A process for obtaining protein heterologous to a yeast organism as a product of yeast expression, processing and secretion which process comprises transforming a yeast organism with an expression vehicle funtionally harboring DNA encoding said protein and a heterologous signal polypeptide, preparing a culture of the transformed organism, growing the culture and recovering said prtein from the medium of the culture.
References Cited (Backward Citations)
No citation records indexed.
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