US5385707A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Metal matrices for use in high gradient magnetic separation of biological materials and method for coating the same
Assignee: Miltenyi Biotec GmbH
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Filing: 1993-01-21
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Granted: 1995-01-31
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Expires: 2013-01-21
Abstract
Abstract Improvements in the existing procedures and materials for conduct of high gradient magnetic separation (HGMS) are disclosed. Superior superparamagnetic particles, optionally coated with a polysaccharide or other, usually organic, materials can be prepared in uniform compositions with homogeneous magnetizations. The coating can conveniently be conjugated to a specific binding moiety complementary to a biological material whose purification or separation is desired. In addition, plastic coated matrices which form superior magnetic gradient-intensifying supports are disclosed, along with improved methods and apparatus to conduct HGMS.
Inventors
- 👤 Stefan Miltenyi
- 👤 Andreas Radbruch
- 👤 Walter Weichel
- 👤 Werner Muller
- 👤 Christoph Gottlinger
- 👤 Klaus L. Meyer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 23:59
CPC Classifications
B; B03; B03C; B03C1/00; B03C1/02; B03C1/025; B03C1/031; B03C1/033; B03C1/034; B01; B01J; B01J20/00; B01J20/02; B01J20/0203; B01J20/0225; B01J20/0229; B01J20/06; B01J20/22; B01J20/24; B01J20/28; B01J20/28002; B01J20/28004; B01J20/28007; B01J20/28009; B01J20/281; B01J20/286; B01J20/30; B01J20/3028; B01J20/3071; B01J20/3078; B01J20/3085; B01J20/3092; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3231; B01J20/3242; B01J20/3268; B01J20/3272; B01J20/3274; B01J20/328; B01J20/3287; B82; B82Y; B82Y30/00; G; G01; G01N; G01N30/00; G01N30/02; G01N30/50; G01N30/52; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; B01J2220/00; B01J2220/50; B01J2220/54; B01J2220/58; G01N2030/022; G01N2030/027; G01N30/56; G01N2030/567; G01N30/88; G01N2030/8809; G01N2030/8813; G01N2400/00; G01N2400/10; G01N2400/12; G01N2400/14; G01N2400/22; G01N2446/00; G01N2446/20; G01N2446/30; G01N2446/80; G01N2446/90; Y; Y10; Y10S; Y10S209/00; Y10S209/932; Y10S435/00; Y10S435/803; Y10S435/8215; Y10S435/822; Y10S435/824; Y10S436/00; Y10S436/803; Y10S436/807
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
174 words
A method to coat a magnetic gradient-intensifying matrix for use in a separation chamber of an apparatus for performing high gradient magnetic separation (HGMS) processes, said apparatus includes a magnet having a pole gap for receiving said separation chamber, said magnet providing a magnetic field in said pole gap, which method includes the steps of: treating said matrix comprised of finely divided magnetically susceptible metal parts with a coat-forming liquid preparation in a preparation vessel, thereby covering the surfaces of said matrix of magnetizable metal parts, which preparation comprises a monomer capable of forming a polymer or a prepolymerized polymer; removing excess coat-forming liquid from said so covered matrix; and effecting the formation of a solid polymer coating of said matrix to form a solid, coated matrix, said solid polymer coating being impermeable to ions and containing substantially less than 30% water by weight based on the total weight of the coating on the matrix to form a coated matrix; transferring said matrix from said preparation vessel to said separation chamber of said apparatus.
Claim 2
Independent Claim
174 words
A method to coat a magnetic gradient-intensifying matrix for use in a separation chamber of an apparatus for performing high gradient magnetic separation (HGMS) processes, said apparatus includes a magnet having a pole gap for receiving said separation chamber, said magnet providing a magnetic field in said pole gap, which method includes the steps of: treating said matrix comprised of finely divided magnetically susceptible metal parts with a coat-forming liquid preparation in a preparation vessel, thereby covering the surfaces of said matrix of magnetizable metal parts, which preparation comprises a monomer capable of forming a polymer or a prepolymerized polymer; removing excess coat-forming liquid from said so covered matrix; and effecting the formation of a solid polymer coating of said matrix to form a solid, coated matrix, said solid polymer coating being impermeable to ions and containing substantially less than 30% water by weight based on the total weight of the coating on the matrix to form a coated matrix; transferring said matrix from said preparation vessel to said separation chamber of said apparatus.
Claim 3
Independent Claim
150 words
A method to coat a magnetic gradient-intensifying matrix for use in a separation chamber of an apparatus for performing high gradient magnetic separation (HGMS) processes, said apparatus includes a magnet having a pole gap for receiving said separation chamber, which method includes the steps of: treating said matrix comprised of finely divided magnetically susceptible metal parts with a coat-forming liquid preparation in said separation chamber, thereby covering the surfaces of said matrix of magnetizable metal parts, which preparation comprises a monomer capable of forming a polymer or a prepolymerized polymer; removing excess coat-forming liquid from said so covered matrix; and effecting the formation of a solid polymer coating of said matrix to form a solid, coated matrix, said solid polymer coating being impermeable to ions and containing substantially less than 30% water by weight based on the total weight of the coating on the matrix to form a coated matrix.
Claim 4
Independent Claim
150 words
A method to coat a magnetic gradient-intensifying matrix for use in a separation chamber of an apparatus for performing high gradient magnetic separation (HGMS) processes, said apparatus includes a magnet having a pole gap for receiving said separation chamber, which method includes the steps of: treating said matrix comprised of finely divided magnetically susceptible metal parts with a coat-forming liquid preparation in said separation chamber, thereby covering the surfaces of said matrix of magnetizable metal parts, which preparation comprises a monomer capable of forming a polymer or a prepolymerized polymer; removing excess coat-forming liquid from said so covered matrix; and effecting the formation of a solid polymer coating of said matrix to form a solid, coated matrix, said solid polymer coating being impermeable to ions and containing substantially less than 30% water by weight based on the total weight of the coating on the matrix to form a coated matrix.
Claim 5
Dependent Claim
24 words
The method of claim 1 or claim 2 wherein said method further including the step of inserting said separation chamber into said pole gap.
Claim 6
Dependent Claim
42 words
The method of claim 1 or claim 2 wherein said magnet provides for a magnetic field in said pole gap, and said matrix consists of fibers of magnetically susceptible material, and wherein said fibers are oriented substantially normal to the magnetic field.
Claim 7
Dependent Claim
65 words
A coated matrix for intensifying locally the magnetic gradient in a separation chamber of an HGMS apparatus in the conduct of HGMS, which coated matrix comprises a metal matrix; and wherein said matrix is magnetized in the presence of a magnetic field, said matrix being coated using a method as claimed in claim 1 or in claim 2, said coated matrix being permeable to liquids.
Claim 8
Dependent Claim
21 words
The coated matrix of claim 5 which includes less than 10% water by weight based on total weight of said coating.
Claim 9
Dependent Claim
21 words
The coated matrix of claim 5 which includes less than 1% water by weight based on total weight of said coating.
Claim 10
Dependent Claim
49 words
The method to conduct HGMS in a separation chamber of an HGMS apparatus, said chamber containing a coated matrix as claimed in claim 5, which method comprises magnetizing said matrix by applying a magnetic field and applying concurrently said sample containing magnetic particles to be separated to said chamber.
Claim 11
Dependent Claim
22 words
The method of claim 8 wherein said coating contains less than 10% water by weight based on total weight of said coating.
Claim 12
Dependent Claim
22 words
The method of claim 8 wherein said coating contains less than 1% water by weight based on total weight of said coating.
Claim 13
Dependent Claim
13 words
The coated matrix of claim 5 wherein said metal matrix is filamentous steel.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-01-30 | 1998-08-01 | 1999-01-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-01-30 | 2002-08-01 | 2003-01-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-01-30 | 2006-08-01 | 2007-01-31 | $7700.00 | Window Closed / Lapsed |