US5378360A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Push column and chromatography apparatus
Assignee: Agilent Technologies Inc
•
Filing: 1993-06-28
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Granted: 1995-01-03
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Expires: 2013-06-28
Abstract
Abstract An apparatus for chromatography of DNA, RNA, proteins and other molecules includes the use of a column adapted to hold a chromatography material and a sample to be filtered. A pneumatic pressure differential is applied across the column and the sample is urged through the chromatography material. A selected portion of the sample may then be collected.
Inventors
- 👤 William D. Huse
- 👤 Anthony M. Sorge
- 👤 Keith V. Sylvester
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 18:25
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/34; C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1003; C12N15/1006; C12N15/101; G; G01; G01N; G01N1/00; G01N1/28; G01N1/40; G01N1/405; G01N30/00; G01N30/02; G01N30/26; G01N30/28; G01N30/32; G01N30/60; G01N30/6047
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
146 words
A chromatography apparatus comprising: a tubular chromatography column including a tube containing a gel chromatography material and a sample, wherein the column has first and second openings therein; a sample of DNA or RNA fragments and unincorporated nucleotides, wherein the DNA or RNA fragments are larger in molecular size than the unincorporated nucleotides; a gel chromatography material having an internal porosity selected intermediate between the molecular size of the DNA or RNA fragments and the molecular size of the unincorporated nucleotides; a syringe attached to the first opening of the column and having a plunger moveable within the syringe that applies pressure sufficient to cause the sample to pass through the gel chromatography material; and a collection vial coupled to the second opening of the column for collecting eluent from the column, wherein the eluent contains said DNA or RNA fragments substantially free of unincorporated nucleotides.
Claim 2
Dependent Claim
146 words
A chromatography apparatus comprising: a tubular chromatography column including a tube containing a gel chromatography material and a sample, wherein the column has first and second openings therein; a sample of DNA or RNA fragments and unincorporated nucleotides, wherein the DNA or RNA fragments are larger in molecular size than the unincorporated nucleotides; a gel chromatography material having an internal porosity selected intermediate between the molecular size of the DNA or RNA fragments and the molecular size of the unincorporated nucleotides; a syringe attached to the first opening of the column and having a plunger moveable within the syringe that applies pressure sufficient to cause the sample to pass through the gel chromatography material; and a collection vial coupled to the second opening of the column for collecting eluent from the column, wherein the eluent contains said DNA or RNA fragments substantially free of unincorporated nucleotides.
Claim 3
Dependent Claim
18 words
A chromatography apparatus as recited in claim 1 wherein the gel chromatography material is a polysaccharide or polyacrylamide.
Claim 4
Dependent Claim
18 words
A chromatography apparatus as recited in claim 1 further comprising support means for supporting said column and syringe.
Claim 5
Dependent Claim
103 words
A chromatography apparatus comprising: a tubular chromatography column including a tube containing a chromatography material and a sample, wherein the column has first and second openings therein; a sample of polyadenylated RNA and nonpolyadenylated RNA; a chromatography material including oligo(dT)-cellulose; a syringe attached to the first opening of the column and having a plunger moveable within the syringe that applies pressure sufficient to cause the sample to pass through the gel chromatography material; and a collection vial coupled to the second opening of the column for collecting eluent from the column, wherein the eluent contains said nonpolyadenylated RNA substantially free of polyadenylated RNA.
Claim 6
Dependent Claim
103 words
A chromatography apparatus comprising: a tubular chromatography column including a tube containing a chromatography material and a sample, wherein the column has first and second openings therein; a sample of polyadenylated RNA and nonpolyadenylated RNA; a chromatography material including oligo(dT)-cellulose; a syringe attached to the first opening of the column and having a plunger moveable within the syringe that applies pressure sufficient to cause the sample to pass through the gel chromatography material; and a collection vial coupled to the second opening of the column for collecting eluent from the column, wherein the eluent contains said nonpolyadenylated RNA substantially free of polyadenylated RNA.
Claim 7
Dependent Claim
18 words
A chromatography apparatus as recited in claim 4 further comprising support means for supporting said column and syringe.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-01-02 | 1998-07-04 | 1999-01-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-01-02 | 2002-07-04 | 2003-01-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-01-02 | 2006-07-04 | 2007-01-03 | $7700.00 | Window Closed / Lapsed |