US5366878A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of site-specific alteration of RNA and production of encoded polypeptides
Assignee: Worcester Foundation for Biomedical Research
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Filing: 1993-03-24
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Granted: 1994-11-22
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Expires: 2013-03-24
Abstract
Abstract A method of site-directed alteration (removal or removal followed by replacement) of selected nucleotides in an RNA molecule, as well as to mixed phosphate backbone oligonucleotides useful in the method. It further relates to a method of producing polypeptides or proteins encoded by the RNA molecule altered by the present method. Through use of the present method, site-directed cleavage of an RNA molecule is effected, followed by excision of the selected or target segment of the RNA molecule.
Inventors
- 👤 Thoru Pederson
- 👤 Sudhir Agrawal
- 👤 Sandra Mayrand
- 👤 Paul C. Zamecnik
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 21:41
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/10; C12N15/102; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C12N15/8222; C12N15/823; C12N15/8233; C12N15/8235; C12N2310/00; C12N2310/30; C12N2310/31; C12N2310/312; C12N2310/3125; C12N2310/314; C12N2310/315
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
152 words
A method of site-specific cleavage of a target RNA segment of an RNA molecule consisting essentially of combining the RNA molecule, in the presence of RNase H, with a mixed phosphate backbone oligonucleotide which is complementary to all or a portion of the RNA molecule wherein the mixed phosphate backbone oligonucleotide has an internal segment which activates RNase H and two flanking nucleotide sequences which are unable to activate RNasc H, one of the two flanking nucleotide sequences being present on either side of the internal segment, and wherein the internucleoside bridging phosphate residues of the internal segment are modified phosphates which are phosphorothioates and the internucleoside bridging phosphate residues of the two flanking modified nucleotide sequences are modified phosphates selected from the group consisting of methyl phosphonates, phosphoromorpholidates, phosphoropiperazidates, and phosphoramidates, under conditions appropriate for hybridization of complementary nucleotide sequences and activation of RNase H, thereby cleaving the target RNA segment.
Claim 2
Independent Claim
152 words
A method of site-specific cleavage of a target RNA segment of an RNA molecule consisting essentially of combining the RNA molecule, in the presence of RNase H, with a mixed phosphate backbone oligonucleotide which is complementary to all or a portion of the RNA molecule wherein the mixed phosphate backbone oligonucleotide has an internal segment which activates RNase H and two flanking nucleotide sequences which are unable to activate RNasc H, one of the two flanking nucleotide sequences being present on either side of the internal segment, and wherein the internucleoside bridging phosphate residues of the internal segment are modified phosphates which are phosphorothioates and the internucleoside bridging phosphate residues of the two flanking modified nucleotide sequences are modified phosphates selected from the group consisting of methyl phosphonates, phosphoromorpholidates, phosphoropiperazidates, and phosphoramidates, under conditions appropriate for hybridization of complementary nucleotide sequences and activation of RNase H, thereby cleaving the target RNA segment.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-11-21 | 1998-05-23 | 1998-11-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-11-21 | 2002-05-23 | 2002-11-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-11-21 | 2006-05-23 | 2006-11-22 | $7700.00 | Window Closed / Lapsed |