US5013830A
Expired / Lapsed
US
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Compounds for the cleavage at a specific position of RNA, oligomers employed for the formation of said compounds, and starting materials for the synthesis of said oligomers
Assignee: Ajinomoto Co Inc
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Filing: 1990-02-12
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Granted: 1991-05-07
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Expires: 2010-02-12
Abstract
Abstract There is disclosed a compound having a double chain which is composed of an RNA (+chain) and a complementary DNA (-chain), wherein a portion of the DNA (-chain) has been replaced by an RNA or a derivative thereof, and wherein, when the compound is subjected to the action of an enzyme having a ribonuclease H activity, it is possible to preferentially cleave the RNA (+chain) in a position corresponding to the unsubstituted portion of the DNA (-chain). The compound can thus be used for the preferential cleavage of a phosphodiester bond in a specific position of RNA. Accordingly, the invention provides a useful means for preparing, for instance, a deletion mutant. There is also disclosed a mixed oligomer which comprises an oligomer of RNA or a derivative thereof and a DNA oligomer, wherein the RNA oligomer or a derivative thereof is conjugated to the DNA oligomer via a phosphate diester linkage between the 5'-hydroxyl group and the 3'-hydroxyl group in the ribose or deoxyribose moiety. There is further disclosed a nucleoside derivative of a given general formula for use as a starting material.
Inventors
- 👤 Eiko Ohtsuka
- 👤 Hideo Inoue
- 👤 Hirokazu Morisawa
- 👤 Susumu Shibahara
- 👤 Sachiko Mukai
- 👤 Tohru Nishihara
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 23:00
CPC Classifications
C; C07; C07H; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/10; C07H19/16; C07H19/20; C07H21/00; C12; C12N; C12N15/00; C12N15/09; C12N15/10
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
88 words
A compound having a double chain and which is composed of an RNA (+chain) and a complementary DNA (-chain), wherein at least one portion of said DNA (-chain) has been replaced by a 2'-O-substituted RNA, wherein said 2'-O-substituted RNA has at least one substituted 2'-hydroxyl group on the ribose moieties of said 2'-O-substituted RNA, and wherein, when said compound is subjected to the action of an enzyme having ribonuclease H activity, the RNA (+chain) is cleaved at a position corresponding to the unreplaced portion of the DNA (-chain).
Claim 2
Dependent Claim
88 words
A compound having a double chain and which is composed of an RNA (+chain) and a complementary DNA (-chain), wherein at least one portion of said DNA (-chain) has been replaced by a 2'-O-substituted RNA, wherein said 2'-O-substituted RNA has at least one substituted 2'-hydroxyl group on the ribose moieties of said 2'-O-substituted RNA, and wherein, when said compound is subjected to the action of an enzyme having ribonuclease H activity, the RNA (+chain) is cleaved at a position corresponding to the unreplaced portion of the DNA (-chain).
Claim 3
Dependent Claim
28 words
The compound of claim 1, wherein said 2'-O-substituted RNA is substituted on at least one 2'-hydroxyl group of the ribose moieties of the RNA by a methyl group.
Claim 4
Dependent Claim
26 words
The compound of claim 1, wherein said 2'-O-substituted RNA is substituted on all hydroxyl groups of the ribose moieties of the RNA by a methyl group.
Claim 5
Dependent Claim
28 words
The compound of claim 1, wherein said 2'-O-substituted RNA is substituted on at least one 2'-hydroxyl group of the ribose moieties of the RNA by an ethyl group.
Claim 6
Dependent Claim
28 words
The compound of claim 1, wherein said 2'-O-substituted RNA is substituted on at least one 2'-hydroxyl group of the ribose moieties of the RNA by a propyl group.
Claim 7
Dependent Claim
63 words
A mixed oligomer which comprises a 2'-O-substituted RNA oligomer and a DNA oligomer, wherein said 2'-O-substituted RNA oligomer has at least one substituted 2'-hydroxyl group on the ribose moieties of said 2'-O-substituted RNA oligomer, wherein said 2'-O-substituted RNA oligomer is conjugated to said DNA oligomer via a phosphodiester linkage between the 5'-hydroxyl group and the 3'-hydroxyl group in the ribose or deoxyribose moiety.
Claim 8
Dependent Claim
63 words
A mixed oligomer which comprises a 2'-O-substituted RNA oligomer and a DNA oligomer, wherein said 2'-O-substituted RNA oligomer has at least one substituted 2'-hydroxyl group on the ribose moieties of said 2'-O-substituted RNA oligomer, wherein said 2'-O-substituted RNA oligomer is conjugated to said DNA oligomer via a phosphodiester linkage between the 5'-hydroxyl group and the 3'-hydroxyl group in the ribose or deoxyribose moiety.
Claim 9
Dependent Claim
30 words
The mixed oligomer of claim 6, wherein said 2'-O-substituted RNA oligomer is substituted on at least one 2'-hydroxyl group of the ribose moieties of the RNA by a methyl group.
Claim 10
Dependent Claim
28 words
The mixed oligomer of claim 6, wherein said 2'-O-substituted RNA oligomer is substituted on all 2'-hydroxyl groups of the ribose moieties of the RNA by a methyl group.
Claim 11
Dependent Claim
30 words
The mixed oligomer of claim 6, wherein said 2'-O-substituted RNA oligomer is substituted on at least one 2'-hydroxyl group of the ribose moieties of the RNA by an ethyl group.
Claim 12
Dependent Claim
30 words
The mixed oligomer of claim 6, wherein said 2'-O-substituted RNA oligomer is substituted on at least one 2'-hydroxyl group of the ribose moieties of the RNA by a propyl group.
Claim 13
Dependent Claim
69 words
A nucleoside compound of the formula: ##STR37## wherein X is a monomethoxy-trityl or dimethoxy-trityl radical, Y represents --P(OCH 3 )--N--(CH(CH 3 ) 2 ) 2 , --P(OCH 2 CH 2 CN)--N--(CH(CH 3 ) 2 ) 2 , or --CO(CH 2 ) m --CO--NH--(CH 2 ) n- (CPG), m and n are each an integer of 1 to 10, and R represents a radical of one of the formulae: ##STR38##
Claim 14
Dependent Claim
69 words
A nucleoside compound of the formula: ##STR37## wherein X is a monomethoxy-trityl or dimethoxy-trityl radical, Y represents --P(OCH 3 )--N--(CH(CH 3 ) 2 ) 2 , --P(OCH 2 CH 2 CN)--N--(CH(CH 3 ) 2 ) 2 , or --CO(CH 2 ) m --CO--NH--(CH 2 ) n- (CPG), m and n are each an integer of 1 to 10, and R represents a radical of one of the formulae: ##STR38##
Claim 15
Dependent Claim
10 words
The nucleoside compound of claim 11, wherein R is: ##STR39##
Claim 16
Dependent Claim
10 words
The nucleoside compound of claim 11, wherein R is: ##STR40##
Claim 17
Dependent Claim
10 words
The nucleoside compound of claim 11, wherein R is: ##STR41##
Claim 18
Dependent Claim
10 words
The nucleoside compound of claim 11, wherein R is: ##STR42##
Claim 19
Dependent Claim
52 words
A mixed oligomer which comprises a 2'-O-substituted RNA oligomer and a DNA oligomer, wherein the 2'-O-substituted RNA is conjugated to the DNA oligomer via a phosphodiester linkage between the 5'-hydroxyl group and the 3'-hydroxyl group in the ribose or deoxyribose moiety, wherein the DNA oligomer is from 3 to 6 bases long.
Claim 20
Dependent Claim
52 words
A mixed oligomer which comprises a 2'-O-substituted RNA oligomer and a DNA oligomer, wherein the 2'-O-substituted RNA is conjugated to the DNA oligomer via a phosphodiester linkage between the 5'-hydroxyl group and the 3'-hydroxyl group in the ribose or deoxyribose moiety, wherein the DNA oligomer is from 3 to 6 bases long.
Claim 21
Dependent Claim
28 words
The mixed oligomer of claim 16, wherein at least one, and up to all, of the 2'-hydroxyl groups in the sugar portion of the RNA has a substituent.
References Cited (Backward Citations)
JPS5644400A
Cited
US4503151A
Cited
US4415732A
Cited
US4401796A
Cited
EP0090789A1
Cited
US4661450A
Cited
JPS604199A
Cited
US4725677A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-05-06 | 1994-11-05 | 1995-05-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-05-06 | 1998-11-05 | 1999-05-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-05-06 | 2002-11-05 | 2003-05-07 | $7700.00 | Window Closed / Lapsed |