US5149797A
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Method of site-specific alteration of rna and production of encoded polypeptides
Assignee: WORCESTER FOUNDATION FOR EXPERIMENTAL BIOLOGY A CORP OF MASSACHUSETTS
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Filing: 1990-02-15
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Granted: 1992-09-22
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Expires: 2010-02-15
Abstract
Abstract A method of site-directed alteration (removal or removal followed by replacement) of selected nucleotides in an RNA molecule, as well as to mixed phosphate backbone oligonucleotides useful in the method. It further relates to a method of producing polypeptides or proteins encoded by the RNA molecule altered by the present method. Through use of the present method, site-directed cleavage of an RNA molecule is effected, followed by excision of the selected or target segment of the RNA molecule.
Inventors
- 👤 Thoru Pederson
- 👤 Sudhir Agrawal
- 👤 Sandra Mayrand
- 👤 Paul C. Zamecnik
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 18:34
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/10; C12N15/102; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C12N15/8222; C12N15/823; C12N15/8233; C12N15/8235; C12N2310/00; C12N2310/30; C12N2310/31; C12N2310/312; C12N2310/3125; C12N2310/314; C12N2310/315
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
92 words
A mixed phosphate backbone oligonucleotide consisting essentially of an internal segment of modified nucleotides which activates RNase H and two modified nucleotide sequences which do not activate RNAse H, in which the two modified nucleotide sequences flank the internal segments, one on each side of the internal segment, wherein the internucleoside bridging phosphate residues of the internal segment are modified phosphates which are phosphorothioates and the internucleoside bridging phosphate residues of the two flanking modified nucleotide sequences are modified phosphate selected from the group consisting of: methyl phosphonates, phosphoromorpholidates, phosphoropiperazidates, and phosphoramidates.
Claim 2
Dependent Claim
92 words
A mixed phosphate backbone oligonucleotide consisting essentially of an internal segment of modified nucleotides which activates RNase H and two modified nucleotide sequences which do not activate RNAse H, in which the two modified nucleotide sequences flank the internal segments, one on each side of the internal segment, wherein the internucleoside bridging phosphate residues of the internal segment are modified phosphates which are phosphorothioates and the internucleoside bridging phosphate residues of the two flanking modified nucleotide sequences are modified phosphate selected from the group consisting of: methyl phosphonates, phosphoromorpholidates, phosphoropiperazidates, and phosphoramidates.
Claim 3
Dependent Claim
24 words
The mixed phosphate backbone oligonucleotide of claim 1, wherein the internucleoside bridging phosphate residues of the two flanking modified nucleotide sequences are methyl phosphonates.
Claim 4
Dependent Claim
23 words
The mixed phosphate backbone oligonucleotide of claim 1, wherein the internucleoside bridging phosphate residues of the two flanking modified nucleotide sequences are phosphoromorpholidates.
Claim 5
Dependent Claim
23 words
The mixed phosphate backbone oligonucleotide of claim 1, wherein the internucleoside bridging phosphate residues of the two flanking modified nucleotide sequences are phosphoropiperazidates.
Claim 6
Dependent Claim
23 words
The mixed phosphate backbone oligonucleotide of claim 1, wherein the internucleoside bridging phosphate residues of the two flanking modified nucleotide sequences are phosphoramidates.
References Cited (Backward Citations)
US4661450A
Cited
US5013830A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-09-22 | 1996-03-23 | 1996-09-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-09-22 | 2000-03-23 | 2000-09-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-09-22 | 2004-03-23 | 2004-09-22 | $7700.00 | Window Closed / Lapsed |