US5360728A
Expired / Lapsed
US
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Modified apoaequorin having increased bioluminescent activity
Assignee: Woods Hole Oceanographic Institution
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Filing: 1992-12-01
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Granted: 1994-11-01
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Expires: 2012-12-01
Abstract
Abstract The present invention encompasses modified apoaequorin nucleotide and amino acid sequences capable of emitting light in the presence of luciferin and a light-triggering cation such as Ca2+, which possess bioluminescent activity greater than unmodified apoaequorin and methods of use therefore.
Inventors
- 👤 Douglas Prasher
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 12:00
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/43504; C07K14/43595; Y; Y10; Y10S; Y10S530/00; Y10S530/855
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
62 words
A composition comprising an isolated altered apoaequorin peptide, wherein at least the amino acid at amino acid position 124 in the amino acid sequence of natural apoaequorin is changed from aspartic acid to serine, said isolated altered apoaequorin, when combined with a luciferin and a light-triggering cation, having greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 2
Dependent Claim
62 words
A composition comprising an isolated altered apoaequorin peptide, wherein at least the amino acid at amino acid position 124 in the amino acid sequence of natural apoaequorin is changed from aspartic acid to serine, said isolated altered apoaequorin, when combined with a luciferin and a light-triggering cation, having greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 3
Dependent Claim
14 words
The composition of claim 1 wherein the altered apoaequorin is SEQ ID NO: 3.
Claim 4
Dependent Claim
62 words
A composition comprising an isolated altered apoaequorin peptide, wherein at least the amino acid at amino acid position 135 in the amino acid sequence of natural apoaequorin is changed from glutamic acid to serine, said isolated altered apoaequorin, when combined with a luciferin and a light-triggering cation, having greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 5
Dependent Claim
62 words
A composition comprising an isolated altered apoaequorin peptide, wherein at least the amino acid at amino acid position 135 in the amino acid sequence of natural apoaequorin is changed from glutamic acid to serine, said isolated altered apoaequorin, when combined with a luciferin and a light-triggering cation, having greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 6
Dependent Claim
14 words
The composition of claim 3 wherein the altered apoaequorin is SEQ ID NO: 4.
Claim 7
Dependent Claim
61 words
A composition comprising an isolated altered apoaequorin peptide, wherein at least the amino acid at amino acid position 129 in the amino acid sequence of natural apoaequorin is changed from glycine to alanine, said isolated altered apoaequorin, when combined with a luciferin and a light-triggering cation, having greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 8
Dependent Claim
61 words
A composition comprising an isolated altered apoaequorin peptide, wherein at least the amino acid at amino acid position 129 in the amino acid sequence of natural apoaequorin is changed from glycine to alanine, said isolated altered apoaequorin, when combined with a luciferin and a light-triggering cation, having greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 9
Dependent Claim
14 words
The composition of claim 5 wherein the altered apoaecluorin is SEQ ID NO: 5.
Claim 10
Dependent Claim
57 words
A composition comprising an isolated altered apoaequorin peptide, wherein at least the amino acid at position 117 of SEQ ID NO:2 is altered from aspartic acid to serine, said isolated altered apoaequorin peptide, when combined with a luciferin and a light-triggering cation, having greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 11
Dependent Claim
57 words
A composition comprising an isolated altered apoaequorin peptide, wherein at least the amino acid at position 117 of SEQ ID NO:2 is altered from aspartic acid to serine, said isolated altered apoaequorin peptide, when combined with a luciferin and a light-triggering cation, having greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 12
Dependent Claim
57 words
A composition comprising an isolated altered apoaequorin peptide, wherein at least the amino acid at position 128 of SEQ ID NO:2 is altered from glutamic acid to serine, said isolated altered apoaecluorin peptide, when combined with a luciferin and a light-triggering cation, having greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 13
Dependent Claim
57 words
A composition comprising an isolated altered apoaequorin peptide, wherein at least the amino acid at position 128 of SEQ ID NO:2 is altered from glutamic acid to serine, said isolated altered apoaecluorin peptide, when combined with a luciferin and a light-triggering cation, having greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 14
Dependent Claim
57 words
A composition comprising an isolated altered apoaequorin peptide, wherein at least the amino acid at position 122 of SEQ ID NO: 2 is altered from glycine to alanine, said isolated altered apoaequorin peptide, when combined with a luciferin and a light-triggering cation, having greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
Claim 15
Dependent Claim
57 words
A composition comprising an isolated altered apoaequorin peptide, wherein at least the amino acid at position 122 of SEQ ID NO: 2 is altered from glycine to alanine, said isolated altered apoaequorin peptide, when combined with a luciferin and a light-triggering cation, having greater bioluminescent activity than natural apoaequorin combined with a luciferin and a light-triggering cation.
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