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US5354447A
Expired / Lapsed
US
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Biosensor and method of quantitative analysis using the same
Assignee: Arkray Inc
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Filing: 1992-12-11
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Granted: 1994-10-11
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Expires: 2012-12-11
Abstract
Abstract A biosensor for electrochemically detecting an electrochemically active material generated by a reaction of a compound to be analyzed in a liquid sample and at least one compound which specifically reacts with the compound to be analyzed, in which a working electrode has at least two electrode parts and the supplied liquid sample contacts the electrode parts successively at an interval, and with which, the electrochemically active material and the compound to be analyzed are quantitatively analyzed separately.
Inventors
- 👤 Harumi Uenoyama
- 👤 Hisashi Okuda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 22:26
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
61 words
A biosensor for electrochemically detecting an electrochemically active material which is generated by a reaction of a compound in a liquid sample to be analyzed and at least a first compound which specifically reacts with said compound to be analyzed, wherein a working electrode has at least two electrode parts and the liquid sample contacts said electrode parts successively in time.
Claim 2
Dependent Claim
61 words
A biosensor for electrochemically detecting an electrochemically active material which is generated by a reaction of a compound in a liquid sample to be analyzed and at least a first compound which specifically reacts with said compound to be analyzed, wherein a working electrode has at least two electrode parts and the liquid sample contacts said electrode parts successively in time.
Claim 3
Dependent Claim
19 words
The biosensor according to claim 1, wherein said first compound is placed on only one of said electrode parts.
Claim 4
Dependent Claim
19 words
The biosensor according to claim 1, wherein a mediator is placed on at least one of said electrode parts.
Claim 5
Dependent Claim
121 words
A method of quantitative analysis of a compound to be analyzed in a liquid sample comprising steps of: supplying the liquid sample on a biosensor claimed in claim 1, detecting an electric signal which depends solely on an electrochemically active material at the first electrode part which the liquid sample contact firstly, after a certain time period from the first contact of the liquid sample with the first electrode part, when the liquid sample reaches the second electrode part, detecting an electric signal which depends on both the electrochemically active material and the compound to be analyzed at the second electrode part, and calculating two electric signals to quantitatively analyze the electrochemically active material and the compound to be analyzed separately.
Claim 6
Dependent Claim
110 words
A sensor for the electrochemical measurement of an analyte and an interfering material contained in a sample consisting essentially of: a single pair of electrodes, said pair having a counter electrode and a measuring electrode, wherein said measuring electrode comprises two portions, a first portion of which reacts electrochemically with said interfering material, and a second portion of which comprises a biologically active material which specifically reacts with an analyte to produce an intermediate which is also electrochemically active with the measuring electrode, wherein said first and second portions of the measuring electrode are joined by a channel which conducts the sample from said first portion to said second portion.
Claim 7
Dependent Claim
110 words
A sensor for the electrochemical measurement of an analyte and an interfering material contained in a sample consisting essentially of: a single pair of electrodes, said pair having a counter electrode and a measuring electrode, wherein said measuring electrode comprises two portions, a first portion of which reacts electrochemically with said interfering material, and a second portion of which comprises a biologically active material which specifically reacts with an analyte to produce an intermediate which is also electrochemically active with the measuring electrode, wherein said first and second portions of the measuring electrode are joined by a channel which conducts the sample from said first portion to said second portion.
Claim 8
Dependent Claim
20 words
The biosensor according to claim 5, wherein said biologically active material is an enzyme, which generates an electrochemically active species.
Claim 9
Dependent Claim
22 words
The biosensor according to claim 5, wherein said analyte is a substance selected from the group consisting of glucose and lactic acid.
Claim 10
Dependent Claim
21 words
The biosensor according to claim 5, wherein said sample is a sample selected from the group of blood, urine and saliva.
Claim 11
Dependent Claim
13 words
The biosensor according to claim 5, wherein said measuring electrode carries a mediator.
Claim 12
Dependent Claim
12 words
The biosensor according to claim 9, wherein said mediator is a ferricyanide.
Claim 13
Dependent Claim
12 words
The biosensor according to claim 10, wherein said ferricyanide is potassium ferricyanide.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-10-10 | 1998-04-11 | 1998-10-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-10-10 | 2002-04-11 | 2002-10-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-10-10 | 2006-04-11 | 2006-10-11 | $7700.00 | Window Closed / Lapsed |