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EP0513804A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Biosensor and method of quantitative analysis using the same

Assignee: Arkray Inc
•
Filing: 1992-05-15
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Granted: —
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Expires: 2012-05-15
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Abstract

Abstract 57 A biosensor which electrochemically detects a material which relates to a reaction of a specific compound in a liquid sample with a biologically active substance or its related substance, in which the biologically active material or an optionally used mediator is placed at a part which is remote from a position of an electrode which acts as electrochemical detector means, and optionally the biological active material or the mediator is covered with a polymer layer.

Inventors

  • 👤 Harumi Uenoyama
  • 👤 Hisashi Okuda

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-10-29 01:20
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; Y; Y10; Y10S; Y10S435/00; Y10S435/817

Claims Analysis

Total Claims: 9
Claim 1
Independent Claim 54 words
A biosensor which electrochemically detects a material which relates to a reaction of a specific compound in a liquid sample with a biologically active substance or its related substance, wherein the biologically active material is placed at a part which is remote from a position of an electrode which acts as electrochemical detector means.
Claim 2
Dependent Claim 54 words
A biosensor which electrochemically detects a material which relates to a reaction of a specific compound in a liquid sample with a biologically active substance or its related substance, wherein the biologically active material is placed at a part which is remote from a position of an electrode which acts as electrochemical detector means.
Claim 3
Dependent Claim 35 words
The biosensor according to claim 1, which comprises a mediator which is placed at a part which is remote from said position of said electrode together with said biologically active substance or its related substance.
Claim 4
Dependent Claim 16 words
The biosensor according to claim 1, which comprises a mediator which is provided on said electrode.
Claim 5
Dependent Claim 23 words
The biosensor according to claim 1, wherein said biologically active substance or its related substance is bound with a hydrophilic polymer and placed.
Claim 6
Dependent Claim 18 words
The biosensor according to claim 3, wherein said mediator is covered with a layer of a hydrophilic polymer.
Claim 7
Dependent Claim 14 words
The biosensor according to claim 1, wherein said biologically active substance is an enzyme.
Claim 8
Dependent Claim 12 words
The biosensor according to claim 6, wherein said enzyme is an oxidoreductase.
Claim 9
Dependent Claim 154 words
A method of quantitative analysis of a material which relates to a reaction of a specific compound in a liquid sample with the biologically active substance or its related substance, the method comprising supplying said liquid sample to a biosensor as claimed in claim 1, reading at least two electrochemical signals from the liquid sample, which are an electrochemical signal at the supply of the sample relating to an electrochemically active substance present in the sample but not to a biologically active substance or its related substance and an electrochemical signal after a sufficient time from the supply of the sample relating to both the biologically active substance or its related substance and the electrochemically active substance present in the sample and operating both signals, whereby the substance which specifically reacts with the biologically active substance or its related substance and the electrochemically active substance present in the sample are separated and quantitatively analyzed.

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