Patent US5324645A successfully refreshed from Google Patents.
US5324645A
Expired / Lapsed
US
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Highly retrovirus-producing DNA construct and cell line
Assignee: Ajinomoto Co Inc
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Filing: 1992-09-10
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Granted: 1994-06-28
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Expires: 2012-09-10
Abstract
Abstract A highly retrovirus-producing DNA construct, which comprises a gene encoding retrovirus which is incorporated into a vector for gene amplification.
Inventors
- 👤 Yoshiyuki Takahara
- 👤 Kumiko Hamada
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 04:52
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/13011; C12N2740/13041; C12N2740/13043; C12N2740/13051; C12N2740/13052
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
31 words
A retrovirus-producing DNA construct, comprising: a gene encoding said retrovirus and a vector for gene amplification, wherein said gene encoding said retrovirus does not contain a retrovirus long terminal repeat sequence.
Claim 2
Dependent Claim
31 words
A retrovirus-producing DNA construct, comprising: a gene encoding said retrovirus and a vector for gene amplification, wherein said gene encoding said retrovirus does not contain a retrovirus long terminal repeat sequence.
Claim 3
Dependent Claim
33 words
The DNA construct of claim 1, wherein said retrovirus encoding gene contains provirus DNA from the initiation codon of the gag domain to the termination codon of the pol domain of said retrovirus.
Claim 4
Dependent Claim
33 words
The DNA construct of claim 1, wherein said retrovirus encoding gene contains provirus DNA from the initiation codon of the env domain to the termination codon of the env domain of said retrovirus.
Claim 5
Dependent Claim
10 words
The DNA construct of claim 1, comprising a metallothionein promoter.
Claim 6
Dependent Claim
14 words
The DNA construct of claim 1, wherein said vector for gene amplification is BMGNeo.
Claim 7
Dependent Claim
11 words
The DNA construct of claim 5, wherein said construct is pGP-KV.
Claim 8
Dependent Claim
11 words
The DNA construct of claim 5, wherein said construct is pENV-KV.
Claim 9
Dependent Claim
37 words
A retrovirus producing helper cell line containing a DNA construct comprising a gene encoding said retrovirus and a vector for gene amplification, wherein said gene encoding said retrovirus does not contain a retrovirus long terminal repeat sequence.
Claim 10
Dependent Claim
37 words
A retrovirus producing helper cell line containing a DNA construct comprising a gene encoding said retrovirus and a vector for gene amplification, wherein said gene encoding said retrovirus does not contain a retrovirus long terminal repeat sequence.
Claim 11
Dependent Claim
13 words
The cell line of claim 8, further comprising a retrovirus vector DNA construct.
Claim 12
Dependent Claim
17 words
The cell line of claim 9, wherein said retrovirus vector DNA construct is pZipNeoSv (DHFR) or N2.
Claim 13
Dependent Claim
15 words
The cell line of claim 10, wherein said retrovirus vector DNA construct is pZipNeoSv (DNFR).
Claim 14
Dependent Claim
21 words
The cell line of claim 8, said cell line containing up to 100 copies of said DNA construct as circular DNA.
References Cited (Backward Citations)
US4650764A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-06-27 | 1997-12-27 | 1998-06-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-06-27 | 2001-12-27 | 2002-06-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-06-27 | 2005-12-27 | 2006-06-28 | $7700.00 | Window Closed / Lapsed |