Patent US5318914A successfully refreshed from Google Patents.
US5318914A
Expired / Lapsed
US
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Process and magnetic device for immunological analysis of a solid phase
Assignee: Bio Rad Innovations SAS
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Filing: 1992-07-21
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Granted: 1994-06-07
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Expires: 2012-07-21
Abstract
Abstract The present invention relates to a process for determining or detecting by immuno adherence a biological substance present in a sample, which consists in introducing the sample into a receptacle on whose walls there is a component which has a specific immunological affinity for the substance to be tested for, in adding magnetic particles on which there is a substance which has a specific immunological affinity for the substance to be tested for, in subjecting them to several successive magnetic actions in order to accelerate the deposition of the said particles onto the walls and to displace those which have not formed specific bonds with the substance to be tested for which adheres to the walls via the component with affinity which is fixed thereto, and in observing the particles deposited.
Inventors
- 👤 Claude Matte
- 👤 Anne Muller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 09:13
CPC Classifications
B; B03; B03C; B03C1/00; B03C1/02; B03C1/28; B03C1/288; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; G01N33/54333; B03C2201/00; B03C2201/26; Y; Y10; Y10S; Y10S436/00; Y10S436/806; Y10S436/824
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
144 words
A process for determining the presence of a biological substance in a sample comprising the steps of: a) introducing the sample into a receptacle having walls on which is immobilized a component which specifically binds the biological substance; b) introducing into the receptacle magnetic particles on which is fixed a substance which binds the biological substance; c) applying a static magnetic field to the particles in a manner such that the particles coat the walls; d) changing the receptacle's position relative to a magnetic field to subject the particles to a field of variable direction in a manner such that the particles not bound to the biological substance are displaced from the walls and collected at the bottom of the receptacle; e) observing the distribution of the magnetic particles in the receptacle as an indication of the presence or absence of the biological substance.
Claim 2
Dependent Claim
144 words
A process for determining the presence of a biological substance in a sample comprising the steps of: a) introducing the sample into a receptacle having walls on which is immobilized a component which specifically binds the biological substance; b) introducing into the receptacle magnetic particles on which is fixed a substance which binds the biological substance; c) applying a static magnetic field to the particles in a manner such that the particles coat the walls; d) changing the receptacle's position relative to a magnetic field to subject the particles to a field of variable direction in a manner such that the particles not bound to the biological substance are displaced from the walls and collected at the bottom of the receptacle; e) observing the distribution of the magnetic particles in the receptacle as an indication of the presence or absence of the biological substance.
Claim 3
Dependent Claim
41 words
The process of claim 1 wherein, following the step d), the process further comprises the step of applying a static magnetic field to the particles in order to remove the particles that are bound to the biological substance on the walls.
Claim 4
Dependent Claim
15 words
The process of claim 1 wherein the receptacle is a well of a microtitration plate.
Claim 5
Dependent Claim
15 words
The process of claim 2 wherein the receptacle is a well of a microtitration plate.
Claim 6
Dependent Claim
18 words
The process according to claim 3 wherein the magnetic particles have diameters between 0.5 μm and 5 μm.
References Cited (Backward Citations)
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US4916081A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-06-06 | 1997-12-06 | 1998-06-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-06-06 | 2001-12-06 | 2002-06-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-06-06 | 2005-12-06 | 2006-06-07 | $7700.00 | Window Closed / Lapsed |