US5316784A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the production of a solid phase matrix coated with an immunologically active substance
Assignee: Roche Diagnostics GmbH
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Filing: 1992-12-21
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Granted: 1994-05-31
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Expires: 2012-12-21
Abstract
Abstract To produce a solid phase coated with an immunologically active substance, a carrier material is absorptively coated with a mixture of an immunologically active substance and a compound which contains at least two functional groups which can be photoactivated and at least one moiety which interacts with the carrier material and the immunologically active substance and it is subsequently irradiated for the cross-linkage.
Inventors
- 👤 Eberhard Maurer
- 👤 Rolf Deeg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 19:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54353
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
131 words
Process for the production of a solid phase matrix coated with an immunologically active substance, comprising the steps of: a) adsorptively coating a carrier material selected from the group consisting of polyamide, polyacrylamide, polyester, polycarbonate, polyvinylchloride, polymethylacrylate, polystyrene and copolymers of polystyrene, with a mixture of (a) an immunologically active substance and (b) a linking or bridging compound which contains at least two functional groups which can be photoactivated and at least one moiety which interacts adsorptively to link the immunologically active substance to the carrier material in the absence of radiation capable of photoactivating said at least two functional groups, thus producing a coated carrier material, and b) subsequently irradiating the coated carrier material to intermolecularly cross-link the immunologically active substance and the carrier material via the linking or bridging.
Claim 2
Dependent Claim
131 words
Process for the production of a solid phase matrix coated with an immunologically active substance, comprising the steps of: a) adsorptively coating a carrier material selected from the group consisting of polyamide, polyacrylamide, polyester, polycarbonate, polyvinylchloride, polymethylacrylate, polystyrene and copolymers of polystyrene, with a mixture of (a) an immunologically active substance and (b) a linking or bridging compound which contains at least two functional groups which can be photoactivated and at least one moiety which interacts adsorptively to link the immunologically active substance to the carrier material in the absence of radiation capable of photoactivating said at least two functional groups, thus producing a coated carrier material, and b) subsequently irradiating the coated carrier material to intermolecularly cross-link the immunologically active substance and the carrier material via the linking or bridging.
Claim 3
Dependent Claim
23 words
Process as claimed in claim 1, wherein the immunologically active substance is selected from the group consisting of antibodies, antigens or binding proteins.
Claim 4
Dependent Claim
35 words
Process as claimed in claims 1 or 2, wherein the immunologically active substance and the linking or bridging compound which can be photoactivated are used in a molar ratio of 1:1 to 1:1×10 6 .
Claim 5
Dependent Claim
29 words
Process as claimed in claims 1 or 2, wherein anti-T4 antibodies, anti-prolactin antibodies, anti-LH antibodies, anti-TSH antibodies, T3-polyhapten, streptavidin, thermo-BSA-streptavidin and/or polystreptavidin are used as the immunologically active substance.
Claim 6
Dependent Claim
20 words
Process as claimed in claims 1 or 2, wherein a homo-bifunctional compound is used as the linking or bridging compound.
Claim 7
Dependent Claim
49 words
Process as claimed in claims 1 or 2 wherein a compound of the general formula is used as the linking or bridging compound in which V and W are the same or different and each denote a functional group which can be photoactivated and Hy denotes a hydrophobic group.
Claim 8
Dependent Claim
57 words
Process as claimed in claims 1 or 2, wherein a compound of the following formula: ##STR2## is used as the linking or bridging compound in which x 1 , x 2 and y 1 , y 2 denote H, NO 2 or a halogen, in each case independently from each other, and Z denotes a spacer group.
Claim 9
Dependent Claim
22 words
Process as claimed in claims 1 or 2, wherein the irradiation is carried out at a wavelength of 250 to 500 nm.
Claim 10
Dependent Claim
22 words
Process as claimed in claims 1 or 2, wherein the irradiation is carried out at a wavelength of 320 to 400 nm.
Claim 11
Dependent Claim
21 words
Process as claimed in claims 1 or 2, wherein the coated solid phase matrix is irradiated for 0.5 to 1 hour.
Claim 12
Dependent Claim
23 words
The process of claim 1 wherein the carrier material is selected from the group consisting of polyvinyl chloride, polymethylacrylate, polystyrene, and polystyrene copolymers.
Claim 13
Dependent Claim
11 words
The process of claim 1, wherein the carrier material is polystyrene.
Claim 14
Dependent Claim
12 words
The process of claim 1, wherein the carrier material is polystyrene-acrylonitrile copolymer.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-05-30 | 1997-11-29 | 1998-05-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-05-30 | 2001-11-29 | 2002-05-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-05-30 | 2005-11-29 | 2006-05-31 | $7700.00 | Window Closed / Lapsed |