US4810638A
Expired / Lapsed
US
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Enzyme-labeled antibody reagent with polyalkyleneglycol linking group
Assignee: Bayer Corp
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Filing: 1986-07-24
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Granted: 1989-03-07
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Expires: 2006-07-24
Abstract
Abstract Enzyme-labeled antibody reagents wherein the enzyme and an antibody reagent, e.g., whole polyclonal or monoclonal antibody or a fragment thereof, are covalently linked through a bis-maleimidopolyalkyleneglycol bridge group. The reagents are useful in immunoassay and other methods for detecting an antigen or hapten that can be bound by the antibody reagent. The conjugates are highly stable and water soluble, and are characterized by a high degree of immunoreactivity and enzyme activity.
Inventors
- 👤 James P. Albarella
- 👤 Robert T. Buckler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 09:01
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6804; C12Q1/6876; C12Q1/6888; C12Q1/689; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; Y; Y10; Y10S; Y10S435/00; Y10S435/964
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
84 words
A water soluble enzyme-labeled antibody reagent comprising an antibody reagent and an enzyme covalently linked thereto through a bis-maleimidopolyalkyleneglycol bridge group covalently bound to sulfhydryl groups, said antibody reagent being IgG or an Fab or Fab' fragment thereof and said bridge group being of the formula: ##STR4## wherein (S) represents sulfhydryl groups in the antibody reagent and enzyme, respectively, to which the bridge group is covalently linked, n is an integer from 2 through 6, and x is an integer from 2 through 12.
Claim 2
Dependent Claim
84 words
A water soluble enzyme-labeled antibody reagent comprising an antibody reagent and an enzyme covalently linked thereto through a bis-maleimidopolyalkyleneglycol bridge group covalently bound to sulfhydryl groups, said antibody reagent being IgG or an Fab or Fab' fragment thereof and said bridge group being of the formula: ##STR4## wherein (S) represents sulfhydryl groups in the antibody reagent and enzyme, respectively, to which the bridge group is covalently linked, n is an integer from 2 through 6, and x is an integer from 2 through 12.
Claim 3
Dependent Claim
10 words
The labeled reagent of claim 1 wherein n is 2.
Claim 4
Dependent Claim
10 words
The labeled reagent of claim 2 wherein x is 5.
Claim 5
Dependent Claim
17 words
The labeled reagent of claim 1 wherein the antibody reagent is an IgG Fab or Fab' fragment.
Claim 6
Dependent Claim
11 words
The labeled reagent of claim 1 wherein the enzyme is β-galactosidase.
Claim 7
Dependent Claim
27 words
The labeled reagent of claim 1 wherein said sulfhydryl groups to which the bridge group is linked are native groups in the antibody reagent and the enzyme.
Claim 8
Dependent Claim
34 words
The labeled reagent of claim 1 wherein one or both of said sulfhydryl groups to which the bridge group is linked have been synthetically introduced into the antibody reagent or the enzyme, or both.
Claim 9
Dependent Claim
27 words
The use of the enzyme-labeled antibody reagent of claim 1 in an immunoassay for detecting an antigen or hapten that can be bound by the antibody reagent.
References Cited (Backward Citations)
US4101380A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-03-06 | 1992-09-05 | 1993-03-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-03-06 | 1996-09-05 | 1997-03-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-03-06 | 2000-09-05 | 2001-03-07 | $7700.00 | Window Closed / Lapsed |