US5164299A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Use of a mixture of conjugated and unconjugated solid phase binding reagent to enhance the performance of assays
Assignee: EIDP Inc
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Filing: 1990-03-20
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Granted: 1992-11-17
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Expires: 2010-03-20
Abstract
Abstract In a solid phase homogeneous or heterogeneous assay for detection or quantitation of an analyte in a biological fluid, use of a combination of unconjugated binding reagent and carrier-conjugated binding reagent immobilized on the solid phase provides enhanced assay performance.
Inventors
- 👤 Stephen B. Lambert
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 21:09
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/576; G01N33/5761; G01N33/5762; G01N33/543; G01N33/54306
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
76 words
A solid phase heterogeneous or homogeneous immunoassay to detect or quantitate an analyte in a liquid sample which comprises: a) reacting binding reagent, immobilized on a solid phase, with sample; and b) determining the extent of binding which occurs in step (a); wherein the immobilized binding reagent is a mixture of carrier-conjugated and unconjugated binding reagent in which the ratio of carrier-conjugated binding reagent to unconjugated binding reagent is in the range from 1:19 to 19:1.
Claim 2
Dependent Claim
76 words
A solid phase heterogeneous or homogeneous immunoassay to detect or quantitate an analyte in a liquid sample which comprises: a) reacting binding reagent, immobilized on a solid phase, with sample; and b) determining the extent of binding which occurs in step (a); wherein the immobilized binding reagent is a mixture of carrier-conjugated and unconjugated binding reagent in which the ratio of carrier-conjugated binding reagent to unconjugated binding reagent is in the range from 1:19 to 19:1.
Claim 3
Dependent Claim
18 words
An immunoassay of claim 1 wherein the analyte and binding reagent are members of an immunochemical binding pair.
Claim 4
Dependent Claim
22 words
Immunoassay of claim 2 wherein the carrier is a protein selected from bovine serum albumin, human serum albumin, ovalbumin, gelatin and casein.
Claim 5
Dependent Claim
52 words
Immunoassay of claim 3 wherein said assay is a competition immunoassay and further wherein the immobilized mixture of carrier protein-conjugated binding reagent and unconjugated binding reagent is contacted with sample and with labeled analyte, then reagents are added to determine the extent of binding of the immobilized binding reagent to labeled analyte.
Claim 6
Dependent Claim
33 words
Competition immunoassay of claim 4 wherein the binding reagent is a mixture of conjugated and unconjugated rHBcAg, the analyte is anti-HBc antibody, and the labeled analyte is enzyme-labeled high affinity monoclonal anti-HBc antibody.
Claim 7
Dependent Claim
34 words
Sandwich immunoassay of claim 1 wherein the immobilized binding reagent is contacted with a liquid sample, then reagents are added to determine the extent of binding of the immobilized binding reagent to the analyte.
Claim 8
Dependent Claim
57 words
Agglutination assay of claim 1 wherein a binding reagent which selectively binds the analyte is immobilized on the surface of a high refractive index particle and the immobilized binding reagent is contacted with a liquid sample and with a reagent which selectively binds the analyte, then the extent of agglutination of the immobilized binding reagent is determined.
Claim 9
Dependent Claim
64 words
Inhibition of agglutination assay of claim 1 wherein a binding reagent which selectively binds a reagent which selectively binds the analyte is immobilized on the surface of a high refractive index particle and the immobilized binding reagent is contacted with a liquid sample and with the reagent which selectively binds the analyte, then the extent of agglutination of the immobilized binding reagent is determined.
Claim 10
Dependent Claim
22 words
Agglutination assay of claim 8 wherein the binding reagent is the same as the analyte or is an analog of the analyte.
Claim 11
Dependent Claim
21 words
An immunoassay according to claim 1 wherein the reacting binding reagent in step (a) is immobilized directly on the solid phase.
Claim 12
Dependent Claim
22 words
An immunoassay according to claim 1 wherein the binding reagent in step (a) is immobilized through an intermediate on the solid phase.
Claim 13
Dependent Claim
23 words
An immunoassay according to claims 10 or 11 wherein another reagent which selectively binds the analyte is additionally reacted with the binding reagent.
Claim 14
Dependent Claim
20 words
An immunoassay according to claims 10, 11 or 12 wherein a labeled analyte is additionally reacted with the binding reagent.
Claim 15
Dependent Claim
40 words
An immunoassay according to claim 1 wherein the immobilized binding reagent is a mixture of carrier-conjugated and unconjugated binding reagent in which the ratio of carrier-conjugated binding reagent to unconjugated binding reagent is in the range from 1:3 to 3:1.
Claim 16
Dependent Claim
40 words
An immunoassay according to claim 1 wherein the immobilized binding reagent is a mixture of carrier-conjugated and unconjugated binding reagent in which the ratio of carrier-conjugated binding reagent to unconjugated binding reagent is in the range from 2:3 to 3:2.
References Cited (Backward Citations)
US4818688A
Cited
EP0282192A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-11-17 | 1996-05-18 | 1996-11-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-11-17 | 2000-05-18 | 2000-11-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-11-17 | 2004-05-18 | 2004-11-17 | $7700.00 | Window Closed / Lapsed |