Abstract In a preferred embodiment, taxol is treated with mesyl chloride to prepare a taxol derivative with a contracted A-ring, which has comparable activity to taxol in a tubulin depolymerization assay, and which shows cytotoxicity against KB cells in a cell culture assay. In an alternate preferred embodiment, taxol is treated with triethyloxonium tetrafluoroborate (Meerwein's reagent) to produce a taxol derivative with an opened oxetane ring. In another alternate preferred embodiment, reaction of taxol with acetyl chloride yields a taxol derivative in which the oxetane ring is opened and the A-ring is contracted. All of the aforementioned products show in vivo activity in KB cell culture assays. Further, the preferred compounds have different in vivo activities, which makes them ideal to form a range of standards for use in biological testing of other compounds.
A taxol compound having the following structure: ##STR1## wherein R1 is C(O)CH 3 , R2 is OH or OSi(CH 2 CH 3 ) 3 , R3 is OSO 2 CH 3 , and R4 is H or Si(CH 2 CH 3 ) 3 .
Claim 2
Dependent Claim
44 words
A taxol compound having the following structure: ##STR1## wherein R1 is C(O)CH 3 , R2 is OH or OSi(CH 2 CH 3 ) 3 , R3 is OSO 2 CH 3 , and R4 is H or Si(CH 2 CH 3 ) 3 .
Claim 3
Dependent Claim
44 words
A process for producing rearranged taxol compounds, comprising the reaction of taxol with an oxonium compound to form a taxol compound with an opened oxetane ring; and reacting said taxol compound with an opened oxetane ring with dimethoxypropane in the presence of p-toluenesulfonic acid.
Claim 4
Dependent Claim
44 words
A process for producing rearranged taxol compounds, comprising the reaction of taxol with an oxonium compound to form a taxol compound with an opened oxetane ring; and reacting said taxol compound with an opened oxetane ring with dimethoxypropane in the presence of p-toluenesulfonic acid.
Claim 5
Dependent Claim
12 words
The process of claim 2, wherein said oxonium compound is triethyloxonium tetrafluoroborate.