Patent US5286789A successfully refreshed from Google Patents.
US5286789A
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Solid phase multiple peptide synthesis
Assignee: Applied Immune Sciences Inc
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Filing: 1993-04-02
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Granted: 1994-02-15
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Expires: 2013-04-02
Abstract
Abstract Methods and compositions are provided, where oligopeptides are produced on a transparent surface while retaining transparency by the cyclical addition of protected monomers. Reagents are specifically selected to allow for efficient reproducible addition, while maintaining transparency of the support. Linkers are provided which permit retention of the oligopeptide to the surface or release of the oligopeptide at completion of the preparation of the oligopeptide. The oligopeptide bound to the support finds use in diagnostic assays, as well as other application, while the free oligopeptides may be used in a variety of ways.
Inventors
- 👤 David Okrongly
- 👤 Brian R. Clark
- 👤 Jack Spesard
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 17:34
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/04; C07K1/042; B; B01; B01J; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00306; B01J2219/00313; B01J2219/00315; B01J2219/00351; B01J2219/00364; B01J2219/00497; B01J2219/00511; B01J2219/00583; B01J2219/00585; B01J2219/0059; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/0061; B01J2219/00614; B01J2219/00617; B01J2219/00623; B01J2219/00626; B01J2219/00659; B01J2219/00718; B01J2219/0072; B01J2219/00725; C40; C40B; C40B40/00; C40B40/04; C40B40/10; C40B60/00; C40B60/14; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
91 words
A fabricated polystyrene article characterized by: being optically clear; being functionalized at high density at an activated polystyrene support article surface with linking groups terminating in a primary amino or hydroxy group, and having linked to said terminating amino or hydroxy group (a) an amino acid the amino group thereof optionally coupled to a protecting group used for peptide synthesis or (b) an oligopeptide, wherein said oligopeptide is substantially uniform in composition and the N-terminal amino group of said oligopeptide is optionally coupled to a protecting group used for peptide synthesis.
Claim 2
Dependent Claim
91 words
A fabricated polystyrene article characterized by: being optically clear; being functionalized at high density at an activated polystyrene support article surface with linking groups terminating in a primary amino or hydroxy group, and having linked to said terminating amino or hydroxy group (a) an amino acid the amino group thereof optionally coupled to a protecting group used for peptide synthesis or (b) an oligopeptide, wherein said oligopeptide is substantially uniform in composition and the N-terminal amino group of said oligopeptide is optionally coupled to a protecting group used for peptide synthesis.
Claim 3
Dependent Claim
23 words
An article according to claim 1, wherein said article is a multiwell plate and is functionalized in a well of said multiwell plate.
Claim 4
Dependent Claim
19 words
An article according to claim 9 wherein said polystyrene is activated with a group which comprises a methyleneaminoacetyl group.
Claim 5
Dependent Claim
26 words
An article according to claim 3 wherein said linking group comprises a thioalkyleneamine group bonded through the thio to said acetyl group of said activating group.
Claim 6
Dependent Claim
27 words
An article according to claims 1 or 4 wherein said linking group is extended with a group comprising a benzyl alcohol group bonded to said terminating group.
Claim 7
Dependent Claim
26 words
An article according to claim 2 wherein all of the linking groups in a well have either all amino or all hydroxy groups in a well.
Claim 8
Dependent Claim
12 words
An article according to claim 1, wherein said article is a container.
Claim 9
Dependent Claim
88 words
A polystyrene bead characterized by: being optically clear; being functionalized at high density at an activated polystyrene bead surface with linking groups terminating in primary amino or hydroxy group; and having linked to said terminating amino or hydroxy group (a) an amino acid the amino group thereof optionally coupled to a protecting group used for peptide synthesis or (b) an oligopeptide, wherein said oligopeptide is substantially uniform in composition and the N-terminal amino group of said oligopeptide is optionally coupled to a protecting group used for peptide synthesis.
Claim 10
Dependent Claim
88 words
A polystyrene bead characterized by: being optically clear; being functionalized at high density at an activated polystyrene bead surface with linking groups terminating in primary amino or hydroxy group; and having linked to said terminating amino or hydroxy group (a) an amino acid the amino group thereof optionally coupled to a protecting group used for peptide synthesis or (b) an oligopeptide, wherein said oligopeptide is substantially uniform in composition and the N-terminal amino group of said oligopeptide is optionally coupled to a protecting group used for peptide synthesis.
Claim 11
Dependent Claim
12 words
An article according to claim 1 wherein the protecting group is fluorenylmethoxycarbonyl.
Claim 12
Dependent Claim
11 words
Beads according to claim 1 wherein the protecting group is fluorenylmethoxycarbonyl.
Claim 13
Dependent Claim
52 words
A fabricated polystyrene article characterized by: being optically clear; being functionalized at high density at an activated polystyrene support article surface with linkage groups terminating in a primary amino or hydroxy group; and having linked to said terminating amino or hydroxy group an oligopeptide, wherein said oligopeptide is substantially uniform in composition.
Claim 14
Dependent Claim
52 words
A fabricated polystyrene article characterized by: being optically clear; being functionalized at high density at an activated polystyrene support article surface with linkage groups terminating in a primary amino or hydroxy group; and having linked to said terminating amino or hydroxy group an oligopeptide, wherein said oligopeptide is substantially uniform in composition.
Claim 15
Dependent Claim
27 words
An article according to claim 1 or 3 wherein said linking group terminates in an aliphatic primary amine, an aliphatic primary hydroxyl, or a benzyl alcohol group.
Claim 16
Dependent Claim
19 words
An article according to claim 1 said linking group is extended by a group comprising a benzyl alcohol group.
Claim 17
Dependent Claim
14 words
An article according to claim 5 wherein said benzyl alcohol group is hydroxymethylphenoxyacetic acid.
Claim 18
Dependent Claim
21 words
An article according to claim 1 or 3 wherein said polystyrene is characterized by having fewer than about 0.1 percent crosslinks.
Claim 19
Dependent Claim
17 words
An article according to claim 1 or 3 wherein said terminating group is a primary amino group.
Claim 20
Dependent Claim
16 words
An article according to claim 1 or 3 wherein said terminating group is a hydroxy group.
Claim 21
Dependent Claim
21 words
An article according to claim 1 or 3 wherein said polystyrene is activated with a group which comprises a methyleneaminoacetyl group.
Claim 22
Dependent Claim
41 words
An article according to claim 2 wherein said activating group comprises a methyleneaminoacetyl group, said linking group is a thioalkyleneamine or hydroxy group optionally further extended with a group comprising a benzyl alcohol group bonded to said amino or hydroxy functionality.
Claim 23
Dependent Claim
19 words
A bead according to claim 8 wherein said polystyrene is activated with a group which comprises a methyleneaminoacetyl group.
Claim 24
Dependent Claim
22 words
A bead according to claim 8 wherein said linking group comprises a thioalkyleneamine group bonded through the thio to said activating group.
Claim 25
Dependent Claim
25 words
A bead according to claim 21 wherein said linking group is extended with a group comprising a benzyl alcohol group bonded to said terminating group.
Claim 26
Dependent Claim
15 words
A bead according to claim 8 wherein said terminating group is a primary amino group.
Claim 27
Dependent Claim
14 words
A bead according to claim 8 wherein said terminating group is a hydroxy group.
Claim 28
Dependent Claim
18 words
A bead according to claim 8 wherein said polystyrene is characterized by fewer than about 0.1 percent crosslinks.
Claim 29
Dependent Claim
41 words
A bead according to claim 8 wherein said activating group comprises a methyleneaminoacetyl group, said linking group is a thioalkyleneamine or hydroxy group optionally further extended with a group comprising a benzyl alcohol group bonded to said amino or hydroxy functionality.
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