US5256536A
Expired / Lapsed
US
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Nucleotide probe for Neisseria gonrrhoeae
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1990-11-09
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Granted: 1993-10-26
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Expires: 2010-11-09
Abstract
Abstract A nucleotide sequence characteristic of Neisseria gonorrhoeae is disclosed. The sequence can be the basis for hybridization type, nucleic acid-based, rapid, in vitro diagnostic assays. The unique nature of the sequence makes it possible to clearly discriminate N. gonorrhoeae from other Neisseria species thus eliminating or substantially reducing the number of false positive readings. A 350 base pair N. gonorrhoeae DNA restriction fragment was cloned after subtractive hybridization to Neisseria meningitidis DNA. In further cloning experiments the sequences adjacent to the original 350 base pair fragment were determined. A portion of this sequence was shown to detect 105 of 106 N. gonorrhoeae strains and no other Neisseria species. In addition to use as detection probes, all or portions of the nucleotide sequence can be used as a ligand for the sandwich capture of N. gonorrhoeae sequences and as primers for in vitro amplification of N. gonorrhoeae sequences. The polypeptides encoded by the presently disclosed sequence, including antibodies thereto, are also disclosed as are their uses.
Inventors
- 👤 Charles G. Miyada
- 👤 Teresa L. Born
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 17:31
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6888; C12Q1/689; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/871
Claims Analysis
Total Claims: 66
Claim 1
Independent Claim
32 words
A sequence of nucleotides specific for Neisseria gonorrhoeae consisting essentially of about seventeen or more contiguous nucleotides of the sequence having the formula of FIG. 1 (SEQ ID NO:1) or its complement.
Claim 2
Dependent Claim
32 words
A sequence of nucleotides specific for Neisseria gonorrhoeae consisting essentially of about seventeen or more contiguous nucleotides of the sequence having the formula of FIG. 1 (SEQ ID NO:1) or its complement.
Claim 3
Dependent Claim
27 words
A polynucleotide probe specific for N. gonorrhoeae which is capable of selectively hybridizing to the DNA sequence of FIG. 1 (SEQ ID NO: 1) or its complement.
Claim 4
Dependent Claim
27 words
A polynucleotide probe specific for N. gonorrhoeae which is capable of selectively hybridizing to the DNA sequence of FIG. 1 (SEQ ID NO: 1) or its complement.
Claim 5
Dependent Claim
15 words
The probe of claim 2 which is complementary to said DNA sequence or its complement.
Claim 6
Dependent Claim
24 words
The probe of claim 2 which is capable of hybridizing to at least seventeen effectively contiguous nucleotides in said DNA sequence or its complement.
Claim 7
Dependent Claim
22 words
The probe of claim 4 wherein the hybridized regions of said probe and said DNA sequence or its complement have 90-100% complementarity.
Claim 8
Dependent Claim
15 words
The probe of claim 2 which consists essentially of at least seventeen effectively contiguous nucleotides.
Claim 9
Dependent Claim
17 words
The probe of claim 6 wherein said probe is complementary to said DNA sequence or its complement.
Claim 10
Dependent Claim
24 words
The probe of claim 6 which is capable of hybridizing to at least seventeen effectively contiguous nucleotides in said DNA sequence or its complement.
Claim 11
Dependent Claim
22 words
The probe of claim 8 wherein the hybridized regions of said probe and said DNA sequence or its complement have 90-100% complementarity.
Claim 12
Dependent Claim
33 words
A conjugate comprising a label bound to a polynucleotide probe specific for N. gonorrhoeae which is capable of selectively hybridizing to the DNA sequence of FIG. 1 (SEQ ID NO:1) or its complement.
Claim 13
Dependent Claim
33 words
A conjugate comprising a label bound to a polynucleotide probe specific for N. gonorrhoeae which is capable of selectively hybridizing to the DNA sequence of FIG. 1 (SEQ ID NO:1) or its complement.
Claim 14
Dependent Claim
17 words
The conjugate of claim 10 wherein said probe is complementary to said DNA sequence or its complement.
Claim 15
Dependent Claim
26 words
The conjugate of claim 10 wherein said probe is capable of hybridizing to at least seventeen effectively contiguous nucleotides in said DNA sequence or its complement.
Claim 16
Dependent Claim
22 words
The conjugate of claim 12 wherein the hybridized regions of said probe and said DNA sequence or its complement have 90-100% complementarity.
Claim 17
Dependent Claim
17 words
The conjugate of claim 10 wherein said probe consists essentially of at least seventeen effectively contiguous nucleotides.
Claim 18
Dependent Claim
22 words
The conjugate of claim 14 wherein the hybridized regions of said probe and said DNA sequence or its complement have 90-100% complementarity.
Claim 19
Dependent Claim
26 words
The conjugate of claim 14 wherein said probe is capable of hybridizing to at least seventeen effectively contiguous nucleotides in said DNA sequence or its complement.
Claim 20
Dependent Claim
22 words
The conjugate of claim 16 wherein the hybridized regions of said probe and said DNA sequence or its complement have 90-100% complementarity.
Claim 21
Independent Claim
84 words
A method for detecting the presence of a gonorrhea infection, comprising the steps of: (a) providing in combination (1) a medium suspected of containing N. gonorrhoeae nucleic acids and (2) at least one polynucleotide probe specific for N. gonorrhoeae which is capable of selectively hybridizing to the DNA sequence of FIG. 1 (SEQ ID NO:1) or its complement, under conditions where complexes of said probe and single stranded N. gonorrhoeae nucleic acids are formed if gonorrhea infection is present; and (b) detecting said complexes.
Claim 22
Independent Claim
84 words
A method for detecting the presence of a gonorrhea infection, comprising the steps of: (a) providing in combination (1) a medium suspected of containing N. gonorrhoeae nucleic acids and (2) at least one polynucleotide probe specific for N. gonorrhoeae which is capable of selectively hybridizing to the DNA sequence of FIG. 1 (SEQ ID NO:1) or its complement, under conditions where complexes of said probe and single stranded N. gonorrhoeae nucleic acids are formed if gonorrhea infection is present; and (b) detecting said complexes.
Claim 23
Dependent Claim
17 words
The method of claim 18 wherein said probe is complementary to said DNA sequence or its complement.
Claim 24
Dependent Claim
26 words
The method of claim 18 wherein said probe is capable of hybridizing to at least seventeen effectively contiguous nucleotides in said DNA sequence or its complement.
Claim 25
Dependent Claim
22 words
The method of claim 20 wherein the hybridized regions of said probe and said DNA sequence or its complement have 90-100% complementarity.
Claim 26
Dependent Claim
17 words
The conjugate of claim 18 wherein said probe consists essentially of at least seventeen effectively contiguous nucleotides.
Claim 27
Dependent Claim
22 words
The method of claim 22 wherein the hybridized regions of said probe and said DNA sequence or its complement have 90-100% complementarity.
Claim 28
Dependent Claim
26 words
The method of claim 22 wherein said probe is capable of hybridizing to at least seventeen effectively contiguous nucleotides in said DNA sequence or its complement.
Claim 29
Dependent Claim
22 words
The method of claim 24 wherein the hybridized regions of said probe and said DNA sequence or its complement have 90-100% complementarity.
Claim 30
Dependent Claim
18 words
The method of claim 18 which further comprises the step of binding said nucleic acids to a support.
Claim 31
Dependent Claim
17 words
The method of claim 18 wherein said probe further comprises a means for rendering said complexes detectable.
Claim 32
Dependent Claim
31 words
The method of claim 27 wherein said means for rendering said complexes detectable includes a label selected from the group consisting of catalysts, enzymes, coenzymes, dyes, fluorescers, quenchers, chemiluminescers and particles.
Claim 33
Dependent Claim
40 words
The method of claim 27 wherein said means for rendering said complexes detectable includes a first specific binding pair (sbp) member bound to said probe and a second sbp member complementary to said sbp first member bound to a label.
Claim 34
Dependent Claim
17 words
The method of claim 18 which further comprises the step of binding said probe to a support.
Claim 35
Dependent Claim
50 words
The method of claim 18 further comprising providing in said combination a second polynucleotide probe which is capable of hybridizing to said DNA or its complement in a region other than that with which said first probe hybridizes, wherein said second probe comprises a means for rendering said complexes detectable.
Claim 36
Dependent Claim
31 words
The method of claim 31 wherein said means for rendering said complexes detectable includes a label selected from the group consisting of catalysts, enzymes, coenzymes, dyes, fluorescers, quenchers, chemiluminescers and particles.
Claim 37
Dependent Claim
40 words
The method of claim 31 wherein said means for rendering said complexes detectable includes a first specific binding pair (sbp) member bound to said probe and a second sbp member complementary to said sbp first member bound to a label.
Claim 38
Independent Claim
81 words
A method for detecting the presence of a gonorrhea infection, comprising the steps of: (a) contacting a sample suspected of containing N. gonorrhoeae nucleic acids with at least one polynucleotide probe specific for N. gonorrhoeae which is capable of selectively hybridizing to the DNA sequence of FIG. 1 (SEQ ID NO: 1) or its complement, under conditions where complexes of said probe and single stranded N. gonorrhoeae nucleic acids are formed if gonorrhea infection is present; and (b) detecting said complexes.
Claim 39
Independent Claim
81 words
A method for detecting the presence of a gonorrhea infection, comprising the steps of: (a) contacting a sample suspected of containing N. gonorrhoeae nucleic acids with at least one polynucleotide probe specific for N. gonorrhoeae which is capable of selectively hybridizing to the DNA sequence of FIG. 1 (SEQ ID NO: 1) or its complement, under conditions where complexes of said probe and single stranded N. gonorrhoeae nucleic acids are formed if gonorrhea infection is present; and (b) detecting said complexes.
Claim 40
Dependent Claim
17 words
The method of claim 34 wherein said probe is complementary to said DNA sequence or its complement.
Claim 41
Dependent Claim
26 words
The method of claim 34 wherein said probe is capable of hybridizing to at least seventeen effectively contiguous nucleotides in said DNA sequence or its complement.
Claim 42
Dependent Claim
22 words
The method of claim 36 wherein the hybridized regions of said probe and said DNA sequence or its complement have 90-100% complementarity.
Claim 43
Dependent Claim
15 words
The method of claim 34 wherein said probe comprises at least seventeen effectively contiguous nucleotides.
Claim 44
Dependent Claim
22 words
The method of claim 38 wherein the hybridized regions of said probe and said DNA sequence or its complement have 90-100% complementarity.
Claim 45
Dependent Claim
26 words
The method of claim 38 wherein said probe is capable of hybridizing to at least seventeen effectively contiguous nucleotides in said DNA sequence or its complement.
Claim 46
Dependent Claim
22 words
The method of claim 40 wherein the hybridized regions of said probe and said DNA sequence or its complement have 90-100% complementarity.
Claim 47
Dependent Claim
10 words
The method of claim 34 wherein said sample is pre-treated.
Claim 48
Dependent Claim
18 words
The method of claim 34 which further comprises the step of binding said nucleic acids to a support.
Claim 49
Dependent Claim
17 words
The method of claim 34 wherein said probe further comprises a means for rendering said complexes detectable.
Claim 50
Dependent Claim
31 words
The method of claim 44 wherein said means for rendering said complexes detectable includes a label selected from the group consisting of catalysts, enzymes, coenzymes, dyes, fluorescers, quenchers, chemiluminescers and particles.
Claim 51
Dependent Claim
40 words
The method of claim 44 wherein said means for rendering said complexes detectable includes a first specific binding pair (sbp) member bound to said probe and a second sbp member complementary to said sbp first member bound to a label.
Claim 52
Dependent Claim
17 words
The method of claim 34 which further comprises the step of binding said probe to a support.
Claim 53
Dependent Claim
50 words
The method of claim 34 further comprising providing in said combination a second polynucleotide probe which is capable of hybridizing to said DNA or its complement in a region other than that with which said first probe hybridizes, wherein said second probe comprises a means for rendering said complexes detectable.
Claim 54
Dependent Claim
31 words
The method of claim 48 wherein said means for rendering said complexes detectable includes a label selected from the group consisting of catalysts, enzymes, coenzymes, dyes, fluorescers, quenchers, chemiluminescers and particles.
Claim 55
Dependent Claim
40 words
The method of claim 48 wherein said means for rendering said complexes detectable includes a first specific binding pair (sbp) member bound to said probe and a second sbp member complementary to said sbp first member bound to a label.
Claim 56
Dependent Claim
73 words
A kit useful in a method for determining the presence of N. gonorrhoeae nucleic acids in a sample suspected of being infected with gonorrhea, which kit comprises in packaged combination: (a) at least one polynucleotide probe specific for N. gonorrhoea which is capable of selectively hybridizing to at least a portion of the DNA sequence of FIG. 1 (SEQ ID NO: 1) or its complement; and (b) means for rendering said probe detectable.
Claim 57
Dependent Claim
73 words
A kit useful in a method for determining the presence of N. gonorrhoeae nucleic acids in a sample suspected of being infected with gonorrhea, which kit comprises in packaged combination: (a) at least one polynucleotide probe specific for N. gonorrhoea which is capable of selectively hybridizing to at least a portion of the DNA sequence of FIG. 1 (SEQ ID NO: 1) or its complement; and (b) means for rendering said probe detectable.
Claim 58
Dependent Claim
17 words
The kit of claim 51 wherein said probe is complementary to said DNA sequence or its complement.
Claim 59
Dependent Claim
26 words
The kit of claim 51 wherein said probe is capable of hybridizing to at least seventeen effectively contiguous nucleotides in said DNA sequence or its complement.
Claim 60
Dependent Claim
22 words
The kit of claim 53 wherein the hybridized regions of said probe and said DNA sequence or its complement have 90-100% complementarity.
Claim 61
Dependent Claim
15 words
The kit of claim 51 wherein said probe comprises at least seventeen effectively contiguous nucleotides.
Claim 62
Dependent Claim
22 words
The kit of claim 55 wherein the hybridized regions of said probe and said DNA sequence or its complement have 90-100% complementarity.
Claim 63
Dependent Claim
26 words
The kit of claim 55 wherein said probe is capable of hybridizing to at least seventeen effectively contiguous nucleotides in said DNA sequence or its complement.
Claim 64
Dependent Claim
22 words
The kit of claim 57 wherein the hybridized regions of said probe and said DNA sequence or its complement have 90-100% complementarity.
Claim 65
Dependent Claim
31 words
The kit of claim 51 wherein said means for rendering said probe detectable includes a label selected from the group consisting of catalysts, enzymes, coenzymes, dyes, fluorescers, quenchers, chemiluminescers and particles.
Claim 66
Dependent Claim
40 words
The kit of claim 55 wherein said means for rendering said probe detectable includes a first specific binding pair (sbp) member bound to said probe and a second sbp member complementary to said first sbp member bound to a label.
References Cited (Backward Citations)
US4358535A
Cited
US4358535B1
Cited
US4446230A
Cited
US4755458A
Cited
EP0227976A2
Cited
US4900659A
Cited
WO1988003957A1
Cited
EP0337896A1
Cited
US5047523A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-10-25 | 1997-04-26 | 1997-10-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-10-25 | 2001-04-26 | 2001-10-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-10-25 | 2005-04-26 | 2005-10-26 | $7700.00 | Window Closed / Lapsed |