Patent US5239171A successfully refreshed from Google Patents.
US5239171A
Expired / Lapsed
US
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Photographing apparatus balancing brightness for microscopes
Assignee: Olympus Corp
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Filing: 1992-02-27
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Granted: 1993-08-24
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Expires: 2012-02-27
Abstract
Abstract A photographing apparatus for microscopes is constructed so that a simultaneous observation is made by overlapping an image of a sample formed by transmitted illumination with that formed by reflected fluorescence, in which signals for designating the simultaneous observation at once cause a second shutter for the transmitted illumination to be closed and a first shutter for the reflected fluorescence to be opened, the brightness of the sample image formed by the reflected fluorescence is measured by a light-detecting element disposed in an optical path for observation, and after a light-adjusting member for the transmitted illumination is controlled so that, based on data outputted from a memory device corresponding to a measured value, the brightness of the sample image by the transmitted illumination in the simultaneous observation is balanced with that of the sample image by the reflected fluorescence, the second shutter is opened. Thus, this photographing apparatus has advantages that whenever the microscopy is switched, an operator can dispense with the need for a fine adjustment of the amount of transmitted illumination light, with the resultant ease of operation, the balance adjustment of brightness in switching is made in a short time, and the bleaching of fluorescence of the sample can be suppressed.
Inventors
- 👤 Yutaka Takabayashi
- 👤 Hiromi Utsunomiya
- 👤 Takashi Nagano
- 👤 Jitsunari Kojima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 10:03
CPC Classifications
G; G02; G02B; G02B21/00; G02B21/36; G02B21/365
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
193 words
A photographing apparatus for microscopes, comprising: a light source for reflected fluorescence illumination; a first shutter disposed in an optical path for reflected fluorescence illumination; a light source for transmitted illumination; a second shutter disposed in an optical path for transmitted illumination; a light-adjusting member disposed in said optical path for transmitted illumination, for adjusting an amount of transmitted light; a light-detecting element disposed in an optical path for observation; and memory means, wherein a simultaneous observation is made by overlapping an image of a sample formed by the transmitted illumination with that formed by the reflected fluorescence, and wherein signals for designating said simultaneous observation at once cause said second shutter to be closed and said first shutter to be opened, brightness of the sample image formed by the reflected fluorescence is measured by said light-detecting element, and after said light-adjusting member is controlled so that, based on data outputted from said memory means corresponding to a measured value, the brightness of the sample image formed by the transmitted illumination in said simultaneous observation is balanced with that of the sample image formed by the reflected fluorescence, said second shutter is opened.
Claim 2
Dependent Claim
193 words
A photographing apparatus for microscopes, comprising: a light source for reflected fluorescence illumination; a first shutter disposed in an optical path for reflected fluorescence illumination; a light source for transmitted illumination; a second shutter disposed in an optical path for transmitted illumination; a light-adjusting member disposed in said optical path for transmitted illumination, for adjusting an amount of transmitted light; a light-detecting element disposed in an optical path for observation; and memory means, wherein a simultaneous observation is made by overlapping an image of a sample formed by the transmitted illumination with that formed by the reflected fluorescence, and wherein signals for designating said simultaneous observation at once cause said second shutter to be closed and said first shutter to be opened, brightness of the sample image formed by the reflected fluorescence is measured by said light-detecting element, and after said light-adjusting member is controlled so that, based on data outputted from said memory means corresponding to a measured value, the brightness of the sample image formed by the transmitted illumination in said simultaneous observation is balanced with that of the sample image formed by the reflected fluorescence, said second shutter is opened.
Claim 3
Dependent Claim
184 words
A photographing apparatus for microscopes, comprising: a light source for reflected fluorescence illumination; a first shutter disposed in an optical path for reflected fluorescence illumination; a light source for transmitted illumination; a second shutter disposed in an optical path for transmitted illumination; a third shutter disposed in an optical path for photography; a light-detecting element disposed in said optical path for photography; and memory means, wherein in multiple-exposure photography based on reflected fluorescence and transmitted light, said first shutter is opened to measure brightness of an image of a sample formed by the reflected fluorescence by means of said light-detecting element, said first shutter is closed when a proper light exposure is reached, a measure value is stored in said memory means, said second shutter is opened to measure the brightness of the sample image formed by the transmitted light by means of said light-detecting element, and when the light exposure is reached which is operated on the basis of the measured value stored in said memory means, one of said second shutter and said third shutter is closed to thereby complete multiple exposure.
Claim 4
Dependent Claim
184 words
A photographing apparatus for microscopes, comprising: a light source for reflected fluorescence illumination; a first shutter disposed in an optical path for reflected fluorescence illumination; a light source for transmitted illumination; a second shutter disposed in an optical path for transmitted illumination; a third shutter disposed in an optical path for photography; a light-detecting element disposed in said optical path for photography; and memory means, wherein in multiple-exposure photography based on reflected fluorescence and transmitted light, said first shutter is opened to measure brightness of an image of a sample formed by the reflected fluorescence by means of said light-detecting element, said first shutter is closed when a proper light exposure is reached, a measure value is stored in said memory means, said second shutter is opened to measure the brightness of the sample image formed by the transmitted light by means of said light-detecting element, and when the light exposure is reached which is operated on the basis of the measured value stored in said memory means, one of said second shutter and said third shutter is closed to thereby complete multiple exposure.
Claim 5
Dependent Claim
44 words
The photographing apparatus according to claim 2, wherein after said first shutter is closed, members constituting said optical path for reflected fluorescence illumination are electrically replaced with members constituting said optical path for transmitted illumination and then exposure on the transmitted light is started.
Claim 6
Dependent Claim
20 words
The photographing apparatus according to claim 2 or 3, wherein a two-dimensional area sensor is used as said light-detecting element.
References Cited (Backward Citations)
US3987463A
Cited
JPS5471652A
Cited
US4475802A
Cited
US4555620A
Cited
US4643540A
Cited
JPS59172618A
Cited
JPS6037538A
Cited
US4756611A
Cited
JPS61185025A
Cited
US4803352A
Cited
US4837595A
Cited
JPS6319810A
Cited
US4897537A
Cited
US5046834A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-08-23 | 1997-02-22 | 1997-08-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-08-23 | 2001-02-22 | 2001-08-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-08-23 | 2005-02-22 | 2005-08-24 | $7700.00 | Window Closed / Lapsed |