US5225062A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Electrophoretic gel for separation and recovery of substances and its use
Assignee: WR Grace and Co Conn
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Filing: 1992-01-28
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Granted: 1993-07-06
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Expires: 2012-01-28
Abstract
Abstract An electrophoretic gel comprising at least one crosslinked temperature-responsive polymeric compound having an LCST is disclosed. A method of separating and recovering substances involves conducting the electrophoresis of a sample containing substances to be separated using the electrophoretic gel at a temperature below the LCST, excising each portion of the gel containing the substance to be separated and raising the temperature of the excised portion to a temperature above the LCST to shrink the excised portion to recover the substances.
Inventors
- 👤 Hiroshi Yoshioka
- 👤 Yuichi Mori
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 12:56
CPC Classifications
B; B01; B01D; B01D57/00; B01D57/02; G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
85 words
An electrophoretic gel for the separation and recovery of substances comprising at least one crosslinked temperature-responsive polymeric compound having an LCST which is ia copolymer decomposable by oxidation or reduction, said copolymer being made of a monomer capable of giving a temperature-responsive polymeric compound selected from the group consisting of N-acryloyl piperidine, N-n-propyl methacrylamide, N-isopropyl acrylamide, N,N-diethyl acrylamide, N-isopropyl methacrylamide, N-cyclopropyl acrylamide, N-acryloyl pyrrolidine, N,N-ethylmethyl acrylamide, N-cyclopropyl methacrylamide and N-ethyl acrylamide and a bifunctional monomer selected from the group consisting of N,N'-diallyltartardiamide, N,N'-(1,2-dihydroxyethylene)-bisacrylamide and N,N'-bisacrylcystamine.
Claim 2
Dependent Claim
85 words
An electrophoretic gel for the separation and recovery of substances comprising at least one crosslinked temperature-responsive polymeric compound having an LCST which is ia copolymer decomposable by oxidation or reduction, said copolymer being made of a monomer capable of giving a temperature-responsive polymeric compound selected from the group consisting of N-acryloyl piperidine, N-n-propyl methacrylamide, N-isopropyl acrylamide, N,N-diethyl acrylamide, N-isopropyl methacrylamide, N-cyclopropyl acrylamide, N-acryloyl pyrrolidine, N,N-ethylmethyl acrylamide, N-cyclopropyl methacrylamide and N-ethyl acrylamide and a bifunctional monomer selected from the group consisting of N,N'-diallyltartardiamide, N,N'-(1,2-dihydroxyethylene)-bisacrylamide and N,N'-bisacrylcystamine.
Claim 3
Dependent Claim
28 words
The gel of claim 1, wherein the amount of said bifunctional monomer is 0.005 to 5% by weight based on the weight of said crosslinked temperature-responsive polymeric compound.
Claim 4
Dependent Claim
11 words
The gel of claim 1, wherein said monomer is N-isopropyl acrylamide.
Claim 5
Dependent Claim
11 words
The gel of claim 1, wherein said bifunctional monomer is N,N'-diallyltartardiamide.
Claim 6
Dependent Claim
11 words
The gel of claim 1, wherein said bifunctional monomer is N,N'-biacrylcystamine.
Claim 7
Dependent Claim
31 words
The gel of claim 1, wherein said copolymer is made of said monomer capable of giving a temperature-responsive polymeric compound, said bifunctional monomer, and a hydrophilic monomer or a hydrophobic monomer.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-07-05 | 1997-01-04 | 1997-07-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-07-05 | 2001-01-04 | 2001-07-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-07-05 | 2005-01-04 | 2005-07-06 | $7700.00 | Window Closed / Lapsed |