Patent US5221620A successfully refreshed from Google Patents.
US5221620A
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Cloning and expression of transforming growth factor β2
Assignee: Oncogen LP
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Filing: 1989-12-05
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Granted: 1993-06-22
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Expires: 2009-12-05
Abstract
Abstract cDNA clones coding for TGF- beta 2 which are used to construct expression vectors capable of directing the high-level expression of mature, biologically active TGF- beta 2, as well as precursor TGF- beta 2 forms, in transfected Chinese Hamster Ovary cells (CHO cells) and transfected COS cells are described. CHO and COS transfectants secreting TGF- beta 2 at high levels are also described. CHO cells transfected with a plasmid vector carrying the complete 414 amino acid simian TGF- beta 2 precursor secrete approximately 5 mu g per ml culture media.
Inventors
- 👤 Anthony F. Purchio
- 👤 Linda Madisen
- 👤 Nancy Webb
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 15:28
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/495; A; A61; A61K; A61K38/00; C07K2319/00
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
57 words
An isolated and purified nucleotide sequence encoding a hybrid transforming growth factor-β1/transforming growth factor-β2 precursor, said nucleotide sequence comprising a simian TGF-β1 precursor coding sequence upstream from and joined in frame with a human TGF-β2 mature coding sequence, as depicted in FIGS. 1D through 1F from about nucleotide residue number -70 to about nucleotide residue number 1755.
Claim 2
Dependent Claim
57 words
An isolated and purified nucleotide sequence encoding a hybrid transforming growth factor-β1/transforming growth factor-β2 precursor, said nucleotide sequence comprising a simian TGF-β1 precursor coding sequence upstream from and joined in frame with a human TGF-β2 mature coding sequence, as depicted in FIGS. 1D through 1F from about nucleotide residue number -70 to about nucleotide residue number 1755.
Claim 3
Dependent Claim
19 words
The cell line CHO 1β9, 12.5, CL 36 as deposited with the ATCC and assigned accession number CRL 9800.
Claim 4
Dependent Claim
19 words
The cell line CHO 1β9, 12.5, CL 36 as deposited with the ATCC and assigned accession number CRL 9800.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-06-21 | 1996-12-21 | 1997-06-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-06-21 | 2000-12-21 | 2001-06-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-06-21 | 2004-12-21 | 2005-06-22 | $7700.00 | Window Closed / Lapsed |