Patent US2967981A successfully refreshed from Google Patents.
EP0200341A1
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EP
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Nucleic acid encoding TGF-beta and its uses
Assignee: Genentech Inc
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Filing: 1986-03-21
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Granted: —
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Expires: 2006-03-21
Abstract
Abstract Nucleic acid encoding TGF-β has been isolated and cloned into vectors which are replicated in bacteria and expressed in eukaryotic cells. TGF-β is recovered from transformed cultures for use in known therapeutic modalities. The labelled nucleic acid is useful in diagnosis.
Inventors
- 👤 Rik Michel Andre Derynck
- 👤 David Vannorman Goeddel
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 23:18
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/495
Claims Analysis
Total Claims: 42
Claim 1
Independent Claim
34 words
A method comprising (a) constructing a vector which includes nucleic acid encoding TGF-β, (b) transforming a host eukaryotic cell with the vector, (c) culturing the transformed cell and (d) recovering TGF-β from the culture.
Claim 2
Independent Claim
34 words
A method comprising (a) constructing a vector which includes nucleic acid encoding TGF-β, (b) transforming a host eukaryotic cell with the vector, (c) culturing the transformed cell and (d) recovering TGF-β from the culture.
Claim 3
Dependent Claim
16 words
The method of claim 1 wherein the eukaryotic cell is a chinese hamster ovary cell line.
Claim 4
Dependent Claim
13 words
The method of claim 1 or claim 2 wherein the TGF-β is preTGF-β.
Claim 5
Dependent Claim
23 words
The method of claim 3 wherein the preTGF-β is a fusion of a viral secretory signal sequence and the sequence of mature TGF-β.
Claim 6
Dependent Claim
19 words
The method of claim 4 wherein the nucleic acid encoding the preTGF-β is operably linked to a viral promoter.
Claim 7
Dependent Claim
12 words
The method of claim 5 wherein the promoter is an SV40 promoter.
Claim 8
Dependent Claim
19 words
The method of claim 1 or claim 2 wherein the TGF-β is mature TGF-β recovered from the culture medium.
Claim 9
Dependent Claim
15 words
DNA encoding TGF-β which is free of one or more introns present in genomic DNA.
Claim 10
Dependent Claim
15 words
DNA encoding TGF-β which is free of one or more introns present in genomic DNA.
Claim 11
Dependent Claim
4 words
Extrachromosomal DNA encoding TGF-β.
Claim 12
Dependent Claim
4 words
Extrachromosomal DNA encoding TGF-β.
Claim 13
Dependent Claim
21 words
mRNA encoding TGF-β of a given species which is cell-free and free of mRNA encoding other proteins of the given species.
Claim 14
Dependent Claim
21 words
mRNA encoding TGF-β of a given species which is cell-free and free of mRNA encoding other proteins of the given species.
Claim 15
Dependent Claim
17 words
The DNA of any one of claims 8 to 10 which is labelled with a detectable moiety.
Claim 16
Dependent Claim
8 words
A replicable vector comprising DNA that encodes TGF-β.
Claim 17
Dependent Claim
8 words
A replicable vector comprising DNA that encodes TGF-β.
Claim 18
Dependent Claim
12 words
The vector of claim 12 wherein the DNA is free of introns.
Claim 19
Dependent Claim
12 words
A host cell containing the vector of claim 12 or claim 13.
Claim 20
Dependent Claim
10 words
The cell of claim 14 which is a eukaryotic cell.
Claim 21
Dependent Claim
15 words
The cell of claim 14 which is a bacterial cell. Claims for contracting state: AT
Claim 22
Independent Claim
34 words
A method comprising (a) constructing a vector which includes nucleic acid encoding TGF-β, (b) transforming a host eukaryotic cell with the vector, (c) culturing the transformed cell and (d) recovering TGF-β from the culture.
Claim 23
Independent Claim
34 words
A method comprising (a) constructing a vector which includes nucleic acid encoding TGF-β, (b) transforming a host eukaryotic cell with the vector, (c) culturing the transformed cell and (d) recovering TGF-β from the culture.
Claim 24
Dependent Claim
16 words
The method of claim 1 wherein the eukaryotic cell is a chinese hamster ovary cell line.
Claim 25
Dependent Claim
13 words
The method of claim 1 or claim 2 wherein the TGF-β is preTGF-β.
Claim 26
Dependent Claim
23 words
The method of claim 3 wherein the preTGF-β is a fusion of a viral secretory signal sequence and the sequence of mature TGF-β.
Claim 27
Dependent Claim
19 words
The method of claim 4 wherein the nucleic acid encoding the preTGF-β is operably linked to a viral promoter.
Claim 28
Dependent Claim
12 words
The method of claim 5 wherein the promoter is an SV40 promoter.
Claim 29
Dependent Claim
19 words
The method of claim 1 or claim 2 wherein the TGF-β is mature TGF-β recovered from the culture medium.
Claim 30
Independent Claim
21 words
A method comprising the production of DNA encoding TGF-β which is free of one or more introns present in genomic DNA.
Claim 31
Independent Claim
21 words
A method comprising the production of DNA encoding TGF-β which is free of one or more introns present in genomic DNA.
Claim 32
Independent Claim
10 words
A method comprising the production of extrachromosomal DNA encoding TGF-β.
Claim 33
Independent Claim
10 words
A method comprising the production of extrachromosomal DNA encoding TGF-β.
Claim 34
Independent Claim
27 words
A method comprising the production of mRNA encoding TGF-β of a given species which is cell-free and free of mRNA encoding other proteins of the given species.
Claim 35
Independent Claim
27 words
A method comprising the production of mRNA encoding TGF-β of a given species which is cell-free and free of mRNA encoding other proteins of the given species.
Claim 36
Independent Claim
19 words
A method of any one of claims 8 to 10 wherein the DNA is labelled with a detectable moiety.
Claim 37
Independent Claim
14 words
A method comprising the production of a replicable vector comprising DNA that encodes TGF-β.
Claim 38
Independent Claim
14 words
A method comprising the production of a replicable vector comprising DNA that encodes TGF-β.
Claim 39
Dependent Claim
12 words
A method of claim 12 wherein the DNA is free of introns.
Claim 40
Dependent Claim
19 words
A method which comprises the preparation of a host cell containing the vector of claim 12 or claim 13.
Claim 41
Dependent Claim
12 words
A method of claim 14 wherein the cell is a eukaryotic cell.
Claim 42
Dependent Claim
12 words
A method of claim 14 wherein the cell is a bacterial cell.
References Cited (Backward Citations)
EP0154434A1
Cited
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