US5221620A
Expired / Lapsed
US
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Cloning and expression of transforming growth factor β2
Assignee: Oncogen LP
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Filing: 1989-12-05
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Granted: 1993-06-22
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Expires: 2009-12-05
Abstract
Abstract cDNA clones coding for TGF- beta 2 which are used to construct expression vectors capable of directing the high-level expression of mature, biologically active TGF- beta 2, as well as precursor TGF- beta 2 forms, in transfected Chinese Hamster Ovary cells (CHO cells) and transfected COS cells are described. CHO and COS transfectants secreting TGF- beta 2 at high levels are also described. CHO cells transfected with a plasmid vector carrying the complete 414 amino acid simian TGF- beta 2 precursor secrete approximately 5 mu g per ml culture media.
Inventors
- 👤 Anthony F. Purchio
- 👤 Linda Madisen
- 👤 Nancy Webb
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 11:34
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/495; A; A61; A61K; A61K38/00; C07K2319/00
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
57 words
An isolated and purified nucleotide sequence encoding a hybrid transforming growth factor-β1/transforming growth factor-β2 precursor, said nucleotide sequence comprising a simian TGF-β1 precursor coding sequence upstream from and joined in frame with a human TGF-β2 mature coding sequence, as depicted in FIGS. 1D through 1F from about nucleotide residue number -70 to about nucleotide residue number 1755.
Claim 2
Dependent Claim
57 words
An isolated and purified nucleotide sequence encoding a hybrid transforming growth factor-β1/transforming growth factor-β2 precursor, said nucleotide sequence comprising a simian TGF-β1 precursor coding sequence upstream from and joined in frame with a human TGF-β2 mature coding sequence, as depicted in FIGS. 1D through 1F from about nucleotide residue number -70 to about nucleotide residue number 1755.
Claim 3
Dependent Claim
19 words
The cell line CHO 1β9, 12.5, CL 36 as deposited with the ATCC and assigned accession number CRL 9800.
Claim 4
Dependent Claim
19 words
The cell line CHO 1β9, 12.5, CL 36 as deposited with the ATCC and assigned accession number CRL 9800.
References Cited (Backward Citations)
US4399216A
Cited
US4338397A
Cited
US4546082A
Cited
EP0105014A2
Cited
EP0128849A1
Cited
EP0155433A1
Cited
WO1985004421A1
Cited
US4708948A
Cited
US4774322A
Cited
EP0200341A1
Cited
EP0268561A2
Cited
EP0293785A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-06-21 | 1996-12-21 | 1997-06-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-06-21 | 2000-12-21 | 2001-06-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-06-21 | 2004-12-21 | 2005-06-22 | $7700.00 | Window Closed / Lapsed |