Patent US5217950A successfully refreshed from Google Patents.
US5217950A
Expired / Lapsed
US
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Nisin compositions for use as enhanced, broad range bactericides
Assignee: Applied Microbiology Inc
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Filing: 1992-01-21
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Granted: 1993-06-08
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Expires: 2012-01-21
Abstract
Abstract Bacteriocin compositions comprising lanthionine containing bacteriocins and non-bactericidal agents. When the bacteriocin compositions are combined with a suitable carrier with each component present in sufficient quantities such that the composition is effective against Gram negative bacteria in addition to Gram positive bacteria, they become enhanced, rapid acting, broad range bactericides suitable for a variety of applications.
Inventors
- 👤 Peter Blackburn
- 👤 June Polak
- 👤 Sara-Ann Gusik
- 👤 Stephen D. Rubino
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 18:59
CPC Classifications
A; A61; A61Q; A61Q11/00; A01; A01N; A01N37/00; A01N37/44; A01N37/46; A01N63/00; A01N63/50; A23; A23B; A23B11/00; A23B11/60; A23B11/65; A23B11/67; A23B2/00; A23B2/70; A23B2/725; A23B2/729; A23B2/7295; A23B2/767; A23B2/783; A23C; A23C9/00; A23C9/152; A23C9/158; A23C9/1585; A61K; A61K8/00; A61K8/18; A61K8/30; A61K8/33; A61K8/37; A61K8/375; A61K8/40; A61K8/41; A61K8/416; A61K8/44; A61K8/64
Claims Analysis
Total Claims: 62
Claim 1
Independent Claim
25 words
A method of synergistically enhancing the bactericidal activity of a lanthionine-containing bacteriocin against gram positive bacteria comprising combining the lanthionine-containing bacteriocin with a suitable chelator.
Claim 2
Independent Claim
25 words
A method of synergistically enhancing the bactericidal activity of a lanthionine-containing bacteriocin against gram positive bacteria comprising combining the lanthionine-containing bacteriocin with a suitable chelator.
Claim 3
Independent Claim
23 words
A method of imparting bactericidal activity against gram negative bacteria to a lanthionine-containing bacteriocin comprising combining the lanthionine-containing bacteriocin with a suitable chelator.
Claim 4
Independent Claim
23 words
A method of imparting bactericidal activity against gram negative bacteria to a lanthionine-containing bacteriocin comprising combining the lanthionine-containing bacteriocin with a suitable chelator.
Claim 5
Independent Claim
29 words
A method of inhibiting the growth of or killing gram negative bacteria comprising simultaneously contacting the bacteria with a suitable amount of a lanthionine-containing bacteriocin and a suitable chelator.
Claim 6
Independent Claim
29 words
A method of inhibiting the growth of or killing gram negative bacteria comprising simultaneously contacting the bacteria with a suitable amount of a lanthionine-containing bacteriocin and a suitable chelator.
Claim 7
Independent Claim
30 words
A method of synergistically inhibiting the growth of or killing gram positive bacteria comprising simultaneously contacting the bacteria with a suitable amount of a lanthionine-containing bacteriocin and a suitable chelator.
Claim 8
Independent Claim
30 words
A method of synergistically inhibiting the growth of or killing gram positive bacteria comprising simultaneously contacting the bacteria with a suitable amount of a lanthionine-containing bacteriocin and a suitable chelator.
Claim 9
Independent Claim
39 words
A method of inhibiting the growth of or killing gram negative and gram positive bacteria which comprises contacting the bacteria with a bactericidal composition comprising an effective amount of a lanthionine-containing bacteriocin, a suitable chelator and a suitable carrier.
Claim 10
Independent Claim
39 words
A method of inhibiting the growth of or killing gram negative and gram positive bacteria which comprises contacting the bacteria with a bactericidal composition comprising an effective amount of a lanthionine-containing bacteriocin, a suitable chelator and a suitable carrier.
Claim 11
Dependent Claim
19 words
The method of claim 1 wherein the bacteriocin is also combined with an effective amount of a suitable surfactant.
Claim 12
Dependent Claim
19 words
The method of claim 2 wherein the bacteriocin is also combined with an effective amount of a suitable surfactant.
Claim 13
Dependent Claim
28 words
The method of claim 1, 2 or 5 wherein the lanthionine-containing bacteriocin is selected from the group consisting of nisin, subtilin, epidermin, cinnamycin, duramycin, ancovenin and Pep 5.
Claim 14
Dependent Claim
11 words
The method of claim 8 wherein the lanthionine-containing bacteriocin is nisin.
Claim 15
Dependent Claim
33 words
The method of claim 1, 2 or 5 wherein the chelator is selected from the group consisting of EDTA, CaEDTA, CaNa 2 EDTA and other alkyldiamine tetraacetates as well as EGTA and citrate.
Claim 16
Dependent Claim
28 words
The method of claim 6 or 7 wherein the surfactant is selected from the group consisting of nonionic surfactants, glycerides, fatty acids, quaternary compounds, emulsifiers and amphoteric surfactants.
Claim 17
Dependent Claim
36 words
The method of claim 5 wherein the concentration of lanthionine-containing bacteriocin is in the range from 0.1 μg/ml to 300 μg/ml and the concentration of chelator is in the range from 0.1 mM to 20 mM.
Claim 18
Dependent Claim
50 words
The method of claim 6 or 7 wherein the concentration of lanthionine-containing bacteriocin is in the range from 0.1 μg/ml to 300 μg/ml, the concentration of chelator is in the range from 0.1 mM to 20 mM and the concentration of surfactant is in the range from 0.01% to 1.0%.
Claim 19
Independent Claim
36 words
A method of inhibiting the growth of or killing gram negative bacteria with a lanthionine-containing bacteriocin which comprises contacting the bacteria simultaneously with an effective amount of nisin and an effective amount of a suitable chelator.
Claim 20
Independent Claim
36 words
A method of inhibiting the growth of or killing gram negative bacteria with a lanthionine-containing bacteriocin which comprises contacting the bacteria simultaneously with an effective amount of nisin and an effective amount of a suitable chelator.
Claim 21
Dependent Claim
10 words
The method of claim 14 wherein the chelator is EDTA.
Claim 22
Dependent Claim
21 words
The method of claim 14 or 15 wherein the bacteria are also contacted simultaneously with an effective amount of a surfactant.
Claim 23
Independent Claim
37 words
A method of preventing contamination of food products by gram negative bacteria which comprises applying to a food product a lanthionine-containing-bacteriocin composition comprising an effective amount of a lanthionine-containing bacteriocin and an effective amount of a chelator.
Claim 24
Independent Claim
37 words
A method of preventing contamination of food products by gram negative bacteria which comprises applying to a food product a lanthionine-containing-bacteriocin composition comprising an effective amount of a lanthionine-containing bacteriocin and an effective amount of a chelator.
Claim 25
Independent Claim
49 words
A method of preventing contamination of food products by gram negative bacteria and more effectively preventing contamination of food products by gram positive bacteria which comprises applying to a food product a lanthionine-containing-bacteriocin composition comprising an effective amount of a lanthionine-containing bacteriocin and an effective amount of a chelator.
Claim 26
Independent Claim
49 words
A method of preventing contamination of food products by gram negative bacteria and more effectively preventing contamination of food products by gram positive bacteria which comprises applying to a food product a lanthionine-containing-bacteriocin composition comprising an effective amount of a lanthionine-containing bacteriocin and an effective amount of a chelator.
Claim 27
Dependent Claim
38 words
The method of claim 17 or 18 wherein the concentration of lanthionine-containing bacteriocin is in the range from 0.1 μg/ml to 300 μg/ml and the concentration of chelator is in the range from 0.1 mM to 20 mM.
Claim 28
Dependent Claim
18 words
The method of claim 17 or 18 wherein the composition further comprises an effective amount of a surfactant.
Claim 29
Dependent Claim
48 words
The method of claim 20 wherein the concentration of lanthionine-containing bacteriocin is in the range from 0.1 μg/ml to 300 μg/ml, the concentration of chelator is in the range from 0.1 mM to 20 mM and the concentration of surfactant is in the range from 0.01% to 1.0%.
Claim 30
Independent Claim
42 words
A method for preventing or treating infections caused by gram negative or gram positive bacteria which comprises applying to a wound, burn or surgical incision a lanthionine-containing-bacteriocin composition comprising an effective amount of lanthionine-containing bacteriocin and an effective amount of a chelator.
Claim 31
Independent Claim
42 words
A method for preventing or treating infections caused by gram negative or gram positive bacteria which comprises applying to a wound, burn or surgical incision a lanthionine-containing-bacteriocin composition comprising an effective amount of lanthionine-containing bacteriocin and an effective amount of a chelator.
Claim 32
Dependent Claim
36 words
The method of claim 22 wherein the concentration of lanthionine-containing bacteriocin is in the range from 0.1 μg/ml to 300 μg/ml and the concentration of chelator is in the range from 0.1 mM to 20 mM.
Claim 33
Dependent Claim
16 words
The method of claim 22 wherein the composition further comprises an effective amount of a surfactant.
Claim 34
Dependent Claim
48 words
The method of claim 24 wherein the concentration of lanthionine-containing bacteriocin is in the range from 0.1 μg/ml to 300 μg/ml, the concentration of chelator is in the range from 0.1 mM to 20 mM and the concentration of surfactant is in the range from 0.01% to 1.0%.
Claim 35
Independent Claim
42 words
A method for preventing or treating conditions caused by gram negative or gram positive bacteria on the skin which comprises applying to the affected skin a lanthionine-containing-bacteriocin composition comprising an effective amount of lanthionine-containing bacteriocin and an effective amount of a chelator.
Claim 36
Independent Claim
42 words
A method for preventing or treating conditions caused by gram negative or gram positive bacteria on the skin which comprises applying to the affected skin a lanthionine-containing-bacteriocin composition comprising an effective amount of lanthionine-containing bacteriocin and an effective amount of a chelator.
Claim 37
Dependent Claim
36 words
The method of claim 26 wherein the concentration of lanthionine-containing bacteriocin is in the range from 0.1 μg/ml to 300 μg/ml and the concentration of chelator is in the range from 0.1 mM to 20 mM.
Claim 38
Dependent Claim
16 words
The method of claim 26 wherein the composition further comprises an effective amount of a surfactant.
Claim 39
Dependent Claim
48 words
The method of claim 28 wherein the concentration of lanthionine-containing bacteriocin is in the range from 0.1 μg/ml to 300 μg/ml, the concentration of chelator is in the range from 0.1 mM to 20 mM and the concentration of surfactant is in the range from 0.01% to 1.0%.
Claim 40
Independent Claim
42 words
A method for preventing or treating conditions caused by gram negative or gram positive bacteria on mucosal epithelia which comprises applying to the affected tissue a lanthionine-containing-bacteriocin composition comprising an effective amount of lanthionine-containing bacteriocin and an effective amount of a chelator.
Claim 41
Independent Claim
42 words
A method for preventing or treating conditions caused by gram negative or gram positive bacteria on mucosal epithelia which comprises applying to the affected tissue a lanthionine-containing-bacteriocin composition comprising an effective amount of lanthionine-containing bacteriocin and an effective amount of a chelator.
Claim 42
Dependent Claim
36 words
The method of claim 30 wherein the concentration of lanthionine-containing bacteriocin is in the range from 0.1 μg/ml to 300 μg/ml and the concentration of chelator is in the range from 0.1 mM to 20 mM.
Claim 43
Dependent Claim
16 words
The method of claim 30 wherein the composition further comprises an effective amount of a surfactant.
Claim 44
Dependent Claim
48 words
The method of claim 32 wherein the concentration of lanthionine-containing bacteriocin is in the range from 0.1 μg/ml to 300 μg/ml, the concentration of chelator is in the range from 0.1 mM to 20 mM and the concentration of surfactant is in the range from 0.01% to 1.0%.
Claim 45
Independent Claim
37 words
A method of sanitizing hard surfaces against gram negative or gram positive bacteria which comprises applying to a hard surface a lanthionine-containing-bacteriocin composition comprising an effective amount of a lanthionine-containing-bacteriocin and an effective amount of a chelator.
Claim 46
Independent Claim
37 words
A method of sanitizing hard surfaces against gram negative or gram positive bacteria which comprises applying to a hard surface a lanthionine-containing-bacteriocin composition comprising an effective amount of a lanthionine-containing-bacteriocin and an effective amount of a chelator.
Claim 47
Dependent Claim
35 words
The method of claim 34 wherein the concentration of lanthionine-containing-bacteriocin is in the range from 0.1 μg/ml to 300 μg/ml and the concentration of chelator is in the range from 0.1 mM to 20 mM.
Claim 48
Dependent Claim
16 words
The method of claim 34 wherein the composition further comprises an effective amount of a surfactant.
Claim 49
Dependent Claim
47 words
The method of claim 36 wherein the concentration of lanthionine-containing-bacteriocin is in the range from 0.1 μg/ml to 300 μg/ml, the concentration of chelator is in the range from 0.1 mM to 20 mM and the concentration of surfactant is in the range from 0.01% to 1.0%.
Claim 50
Independent Claim
31 words
A method of inhibiting the growth of or killing Salmonella typhimurium which comprises contacting the bacteria with an effective amount of a composition comprising the lanthionine-containing-bacteriocin nisin and a suitable chelator.
Claim 51
Independent Claim
31 words
A method of inhibiting the growth of or killing Salmonella typhimurium which comprises contacting the bacteria with an effective amount of a composition comprising the lanthionine-containing-bacteriocin nisin and a suitable chelator.
Claim 52
Independent Claim
31 words
A method of inhibiting the growth of or killing Escherichia coli which comprises contacting the bacteria with an effective amount of a composition comprising the lanthionine-containing-bacteriocin nisin and a suitable chelator.
Claim 53
Independent Claim
31 words
A method of inhibiting the growth of or killing Escherichia coli which comprises contacting the bacteria with an effective amount of a composition comprising the lanthionine-containing-bacteriocin nisin and a suitable chelator.
Claim 54
Independent Claim
31 words
A method of inhibiting the growth of or killing Pseudomonas aeruginosa which comprises contacting the bacteria with an effective amount of a composition comprising the lanthionine-containing-bacteriocin nisin and a suitable chelator.
Claim 55
Independent Claim
31 words
A method of inhibiting the growth of or killing Pseudomonas aeruginosa which comprises contacting the bacteria with an effective amount of a composition comprising the lanthionine-containing-bacteriocin nisin and a suitable chelator.
Claim 56
Independent Claim
31 words
A method of inhibiting the growth of or killing Bacteroides gingivalis which comprises contacting the bacteria with an effective amount of a composition comprising the lanthionine-containing-bacteriocin nisin and a suitable chelator.
Claim 57
Independent Claim
31 words
A method of inhibiting the growth of or killing Bacteroides gingivalis which comprises contacting the bacteria with an effective amount of a composition comprising the lanthionine-containing-bacteriocin nisin and a suitable chelator.
Claim 58
Independent Claim
31 words
A method of inhibiting the growth of or killing Actinobacillus actinomycetescomitans which comprises contacting the bacteria with an effective amount of a composition comprising the lanthionine-containing-bacteriocin nisin and a suitable chelator.
Claim 59
Independent Claim
31 words
A method of inhibiting the growth of or killing Actinobacillus actinomycetescomitans which comprises contacting the bacteria with an effective amount of a composition comprising the lanthionine-containing-bacteriocin nisin and a suitable chelator.
Claim 60
Independent Claim
31 words
A method of inhibiting the growth of or killing Klebsiella pneumoniae which comprises contacting the bacteria with an effective amount of a composition comprising the lanthionine-containing-bacteriocin nisin and a suitable chelator.
Claim 61
Independent Claim
31 words
A method of inhibiting the growth of or killing Klebsiella pneumoniae which comprises contacting the bacteria with an effective amount of a composition comprising the lanthionine-containing-bacteriocin nisin and a suitable chelator.
Claim 62
Independent Claim
40 words
The method of any one of claims 14 and 38-43 wherein the concentration of nisin is in the range from 0.1 μg/ml to 300 μg/ml and the concentration of chelator is in the range from 0.1 mM to 20 mM.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-06-07 | 1996-12-07 | 1997-06-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-06-07 | 2000-12-07 | 2001-06-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-06-07 | 2004-12-07 | 2005-06-08 | $7700.00 | Window Closed / Lapsed |