US5203992A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Apparatus for optimizing the liquid chromatographic separation of a sample
Assignee: Agilent Technologies Inc
•
Filing: 1992-03-02
•
Granted: 1993-04-20
•
Expires: 2012-03-02
Abstract
Abstract An apparatus for optimizing the liquid chromatographic separation of a sample comprises means for performing liquid chromatographic separations at selectable values of an optimization parameter, such as the composition of the mobile phase. Based upon measured chromatographic data of the sample and upon a mathematical model, the retention behavior of the sample as a function of the optimization parameter is derived by data processing means and displayed on a display means. An input means permits the chromatographer to select from the displayed retention behavior a desired value of the optimization parameter. In response to such a selection, the resulting chromatogram for this value is calculated and displayed. By means of the displayed retention behavior and the corresponding chromatograms, the chromatographer can quickly locate a value of the optimization parameter for which optimal results can be expected. Then, a chromatographic separation of the sample is performed with the selected value. The chromatographic data obtained in that separation are used to refine the mathematical model from which a new retention behavior is derived. The process is iterated until a desired optimum is achieved.
Inventors
- 👤 Antonius Drouen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 03:29
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/02; G01N30/26; G01N30/28; G01N30/34; G01N30/86; G01N30/8658
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
87 words
An apparatus for optimizing the liquid chromatographic separation of a sample comprising a plurality of components with respect to an optimization parameter, comprising: means for performing liquid chromatographic separations at selected values of the optimization parameter; data processing means programmed to: derive retention behavior of each component as a function of the optimization parameter; and derive chromatograms corresponding to selectable values of the optimization parameter; and display means for displaying the derived retention behavior as a function of the optimization parameter and for displaying the derived chromatograms.
Claim 2
Independent Claim
87 words
An apparatus for optimizing the liquid chromatographic separation of a sample comprising a plurality of components with respect to an optimization parameter, comprising: means for performing liquid chromatographic separations at selected values of the optimization parameter; data processing means programmed to: derive retention behavior of each component as a function of the optimization parameter; and derive chromatograms corresponding to selectable values of the optimization parameter; and display means for displaying the derived retention behavior as a function of the optimization parameter and for displaying the derived chromatograms.
Claim 3
Dependent Claim
53 words
The apparatus of claim 1, further comprising controller means for initiating a further liquid chromatographic separation of the sample at a selected value of the optimization parameter, said further separation being initiated in response to the selection of the specific value from the values of the optimization parameter displayed on the display means.
Claim 4
Dependent Claim
34 words
The apparatus of claim 2, wherein the data processing means is programmed to derive retention behavior as a function of the optimization parameter using the retention behavior measured in the further liquid chromatographic separation.
Claim 5
Dependent Claim
66 words
The apparatus of claim 1, further comprising marking means displayed and movable on the display means for selecting certain values of the optimization parameter from the display of the retention behavior, wherein the positioning of the marking means at a certain value of the optimization parameter causes the corresponding chromatogram to be derived by the data processing means and to be displayed by the display means.
Claim 6
Dependent Claim
54 words
The apparatus of claim 1, wherein the data processing means is programmed to derive, from the retention behavior, at least one separation quality factor for the displayed values of the optimization parameter, and wherein the display means display a graphical representation of the separation quality factor simultaneously with the display of the retention behavior.
Claim 7
Dependent Claim
17 words
The apparatus of claim 1 wherein retention behavior comprises retention time for at least one sample component.
Claim 8
Dependent Claim
29 words
The apparatus of claim 6, wherein the display of the retention behavior comprises a graphical representation of retention time of at least one sample component versus the optimization parameter.
Claim 9
Dependent Claim
18 words
The apparatus of claim 6 wherein retention behavior further comprises the width of at least one chromatographic peak.
Claim 10
Dependent Claim
23 words
The apparatus of claim 8, comprising means for deriving the width of at least one chromatographic peak as a function of retention time.
Claim 11
Dependent Claim
48 words
The apparatus of claim 1, wherein the data processing means derives retention behavior according to: ln(k.sub.i)=(a·X)+b wherein k i is the chromatographic capacity factor, X is the optimization parameter, and a and b are constants, and wherein derived retention behavior are obtained by interpolating between measured retention behavior.
Claim 12
Dependent Claim
20 words
The apparatus of claim 1, wherein means for performing the liquid chromatographic separation comprises a mobile phase having known composition.
Claim 13
Dependent Claim
17 words
The apparatus of claim 11, wherein the optimization parameter is the relative composition of the mobile phase.
Claim 14
Dependent Claim
14 words
The apparatus of claim 1, wherein the selectable values are between the selected values.
References Cited (Backward Citations)
US3701609A
Cited
US4066879A
Cited
US4063077A
Cited
US4116046A
Cited
US4237422A
Cited
US4357668A
Cited
US4364263A
Cited
US4927532A
Cited
US4592842A
Cited
US4719017A
Cited
US4595496A
Cited
US4579663A
Cited
US4595495A
Cited
US4762617A
Cited
US4886590A
Cited
US4802981A
Cited
US4969993A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-04-19 | 1996-10-19 | 1997-04-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-04-19 | 2000-10-19 | 2001-04-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-04-19 | 2004-10-19 | 2005-04-20 | $7700.00 | Window Closed / Lapsed |