US4357668A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Base line correction method and apparatus
Assignee: Applied Biosystems Inc
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Filing: 1980-03-04
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Granted: 1982-11-02
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Expires: 2000-03-04
Abstract
Abstract In a solvent gradient liquid chromatography system, base line shift is compensated by operating the system in a calibrate cycle with a desired solvent gradient program while subtracting and recording in digital form correction factors sufficient to maintain a flat base line at timed intervals during the program, and then successively recalling said digital correction factors at corresponding time intervals during a run cycle with a test sample and converting the correction factors to analog form and subtracting the converted correction factors from the system output signal for base line correction.
Inventors
- 👤 Arnold Schwartz
- 👤 Edward B. Delany
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 14:27
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/02; G01N30/86; G01N30/8651; G01N30/8624; G01N30/8641; H; H03; H03K; H03K5/00; H03K5/01; H03K5/08; H03K5/082; H03K5/084
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
217 words
A liquid chromatography system for performing programmable solvent gradient scans, comprising a liquid chromatograph, a spectrophotometer connected to said chromatograph, and apparatus for compensating for base line shift as the proportions of solvents are changed comprising a digital data memory for storing a series of digital numbers representing the required values of base line shift correction signals at spaced time intervals during a solvent gradient program, control means for reading out said digital numbers in sequence at spaced time intervals, a digital-to-analog converter connected to receive said base line shift correction signal numbers and to convert each number to a corresponding base line correction signal voltage, a sample-and-hold circuit, switch means for connecting the voltage from said digital-to-analog converter to said sample-and-hold circuit at spaced time intervals as different base line shift correction signals are available at the output of said digital-to-analog converter, and an offset amplifier having at least two input signals, one input signal to said offset amplifier being received from said spectrophotometer, the other input signal of said offset amplifier being received from said sample-and-hold circuit for subtracting the base line shift correction signals stored within said sample-and-hold circuit from the signals received from said spectrophotometer, the output of said offset amplifier comprising the base line shift corrected output of said liquid chromatography system.
Claim 2
Dependent Claim
217 words
A liquid chromatography system for performing programmable solvent gradient scans, comprising a liquid chromatograph, a spectrophotometer connected to said chromatograph, and apparatus for compensating for base line shift as the proportions of solvents are changed comprising a digital data memory for storing a series of digital numbers representing the required values of base line shift correction signals at spaced time intervals during a solvent gradient program, control means for reading out said digital numbers in sequence at spaced time intervals, a digital-to-analog converter connected to receive said base line shift correction signal numbers and to convert each number to a corresponding base line correction signal voltage, a sample-and-hold circuit, switch means for connecting the voltage from said digital-to-analog converter to said sample-and-hold circuit at spaced time intervals as different base line shift correction signals are available at the output of said digital-to-analog converter, and an offset amplifier having at least two input signals, one input signal to said offset amplifier being received from said spectrophotometer, the other input signal of said offset amplifier being received from said sample-and-hold circuit for subtracting the base line shift correction signals stored within said sample-and-hold circuit from the signals received from said spectrophotometer, the output of said offset amplifier comprising the base line shift corrected output of said liquid chromatography system.
Claim 3
Dependent Claim
192 words
Apparatus as claimed in claim 1 further comprising: a comparison amplifier having at least two inputs; and wherein said switch means is operable to disconnect said digital-to-analog converter from said sample-and-hold circuit when conversion of the output of said offset amplifier to digital numbers is made for display, said switch means being operable to connect said digital-to-analog converter to one input of said comparison amplifier when disconnecting said digital-to-analog converter from said sample-and-hold circuit, the other input of said comparison amplifier being connected to receive the output of said offset amplifier, said control means being operable in a successive approximation mode to cause said digital-to-analog converter to have a digital input value which provides an output signal voltage which balances with said system output voltage at said comparision amplifier so that the digital input signal to said digital-to-analog converter corresponds in magnitude to said system output voltage, said control means being operable to convert the balanced digital input value supplied to said digital-to-analog converter to a corresponding decimal number for display, and a decimal display unit connected to receive and display the decimal number as visible indication of the system output signal.
Claim 4
Dependent Claim
208 words
A system as claimed in claim 1 wherein there is included a comparison amplifier having at least two inputs, said apparatus being operable in a calibrate mode, said switch means being operable when in the cailbrate mode to connect said digital-to-analog converter to said sample-and-hold circuit, said switch means being operable when in the calibrate mode to connect one input of said comparison amplifier to ground, the other input of said comparison amplifier being connected to receive the output of said offset amplifier, and said control means being operable by use of said comparison amplifier in a successive approximation mode to cause said digital-to-analog converter to have a digital input value which provides an output signal voltage which balances with said system output voltage so that the digital input signal to said digital-to-analog converter corresponds in magnitude to said system output voltage during a calibrate cycle when the system is run without a sample, said control means being operable to store the balanced digital input value supplied to said digital-to-analog converter in said digital memory as a base line shift correction signal number, and said control means being operable to repeat the above-mentioned operation at spaced time intervals to store a series of base line shift correction numbers.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-11-01 | 1986-05-03 | 1986-11-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-11-01 | 1990-05-03 | 1990-11-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-11-01 | 1994-05-03 | 1994-11-02 | $7700.00 | Window Closed / Lapsed |