Patent US5182196A successfully refreshed from Google Patents.
US5182196A
Expired / Lapsed
US
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Expression systems for overproduction of desired proteins
Assignee: Biogen Inc
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Filing: 1989-06-27
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Granted: 1993-01-26
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Expires: 2009-06-27
Abstract
Abstract Recombinant DNA molecules comprising DNA sequences derived from bacteriophage T4 that are useful in expressing desired polypeptides in unexpectedly high yields, hosts and expression systems comprising such recombinant DNA molecules and methods for expressing desired polypeptides in high yields by the utilization of such hosts and expression systems.
Inventors
- 👤 Bernard Allet
- 👤 Eric H. Kawashima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 23:05
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; A; A61; A61K; A61K38/00; A61K38/16; A61K38/17; A61K38/19; A61K38/191; A61K38/21; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/525
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
119 words
A method for improving the expression of a non-bacteriophage T4 protein comprising culturing a prokaryotic host transformed with a recombinant DNA molecular capable of directing expression of said non-bacteriophase T4 protein in high yields in said prokaryotic host, said recombinant DNA molecule comprising the following elements, operatively linked in the following order, 5' to 3': DNA sequence I, DNA sequence X, DNA sequence coding for a non-bacteriophage T4 protein, wherein said DNA sequence I consists of: ##STR9## said DNA sequence X comprises a DNA sequence selected from the group consisting of CGATACT, CGCGATACT, ATACTAAA, ATACT, OGCGATACTAAA, CGATACTAA and CGATTCC and said non-bacteriophase T4 protein is selected from the group consisting of human interleukin 2 and human tumor necrosis factor.
Claim 2
Independent Claim
119 words
A method for improving the expression of a non-bacteriophage T4 protein comprising culturing a prokaryotic host transformed with a recombinant DNA molecular capable of directing expression of said non-bacteriophase T4 protein in high yields in said prokaryotic host, said recombinant DNA molecule comprising the following elements, operatively linked in the following order, 5' to 3': DNA sequence I, DNA sequence X, DNA sequence coding for a non-bacteriophage T4 protein, wherein said DNA sequence I consists of: ##STR9## said DNA sequence X comprises a DNA sequence selected from the group consisting of CGATACT, CGCGATACT, ATACTAAA, ATACT, OGCGATACTAAA, CGATACTAA and CGATTCC and said non-bacteriophase T4 protein is selected from the group consisting of human interleukin 2 and human tumor necrosis factor.
Claim 3
Dependent Claim
49 words
The method according to claim 1, said DNA molecular further comprising a non-bacteriophage T4 promoter, said recombinant DNA molecule comprising the following elements, operatively linked in the following order, 5' to 3'; non-bacteriophage T4 promoter, DNA sequence I, DNA sequence X, DNA sequence coding for a non-bacteriophage T4 protein.
Claim 4
Dependent Claim
14 words
The method according to claim 2, wherein said promoter is a P L promoter.
Claim 5
Dependent Claim
52 words
The method according to claim 1, said DNA molecular further comprising bacteriophage T4 transcription terminator sequence, said recombinant DNA molecule comprising the following elements, operatively linked in the following order, 5' to 3': DNA sequence I, DNA sequence X, DNA sequence coding for a non-bacteriophage T4 protein, bacteriophage T4 transcription terminator sequence.
Claim 6
Dependent Claim
17 words
The method according to claim 4, wherein said T4 transcription terminator sequence comprises the DNA sequence: ##STR10##
Claim 7
Independent Claim
22 words
The method of any one of claims 3, 5, 2, 4, and 1 wherein said host is not infected by bacteriophage T4.
Claim 8
Dependent Claim
99 words
A recombinant DNA molecule comprising the following elements, operatively linked in the following order, 5' to 3': DNA sequence I, DNA sequence X, DNA sequence coding for a non-bacteriophage T4 protein, wherein said DNA sequence I consists of: ##STR11## said DNA sequence X comprises a DNA sequence selected from the group consisting of CGATACT, CGCGATACT, ATACTAAA, ATACT, CGCGATACTAA, CGATACTAAA and CGATTCC and said non-bacteriophage T4 protein is selected from the group consisting of human interleukin 2 and human tumor necrosis factor, said recombinant DNA molecule directing expression of said non-bacteriophage T4 protein in high yields in a prokaryotic host.
Claim 9
Dependent Claim
99 words
A recombinant DNA molecule comprising the following elements, operatively linked in the following order, 5' to 3': DNA sequence I, DNA sequence X, DNA sequence coding for a non-bacteriophage T4 protein, wherein said DNA sequence I consists of: ##STR11## said DNA sequence X comprises a DNA sequence selected from the group consisting of CGATACT, CGCGATACT, ATACTAAA, ATACT, CGCGATACTAA, CGATACTAAA and CGATTCC and said non-bacteriophage T4 protein is selected from the group consisting of human interleukin 2 and human tumor necrosis factor, said recombinant DNA molecule directing expression of said non-bacteriophage T4 protein in high yields in a prokaryotic host.
Claim 10
Dependent Claim
46 words
The recombinant DNA molecule of claim 7, further comprising a non-bacteriophage T4 promoter, said recombinant DNA molecule comprising the following elements, operatively linked in the following order, 5' to 3': non-bacteriophage T4 promoter, DNA sequence I, DNA sequence X, DNA sequence coding for anon-bacteriophage T4 protein.
Claim 11
Dependent Claim
15 words
The recombinant DNA molecule of claim 8, wherein said promoter is a P L promoter.
Claim 12
Dependent Claim
55 words
The recombinant DNA molecule of claim 7, further comprising a bacteriophage T 4 transcription terminator sequence, said recombinant DNA molecule comprising the following elements, operatively linked in the following order, 5' to 3': DNA sequence I, DNA sequence X, DNA sequence coding for a non-bacteriophage T4 protein and a bacteriophage T 4 transcription terminator sequence.
Claim 13
Dependent Claim
18 words
The recombinant DNA molecule of claim 10, wherein said T4 transcription terminator sequence comprises the DNA sequence: ##STR12##
Claim 14
Dependent Claim
20 words
A procaryotic host transformed with the recombinant DNA molecule of any one of claims 9, 11, 7, 8, and 10.
Claim 15
Dependent Claim
16 words
A prokaryotic host according to claim 12, wherein said host is not infected by bacteriophage T4.
References Cited (Backward Citations)
EP0041767A2
Cited
EP0118977A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-01-26 | 1996-07-27 | 1997-01-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-01-26 | 2000-07-27 | 2001-01-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-01-26 | 2004-07-27 | 2005-01-26 | $7700.00 | Window Closed / Lapsed |