EP0041767A2
Expired / Lapsed
EP
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Improved vectors and methods for making such vectors and for expressing cloned genes
Assignee: Biogen Inc
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Filing: 1981-04-01
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Granted: —
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Expires: 2001-04-01
Abstract
Abstract Improved vectors and methods for expressing cloned genes of prokaryotic or eukaryotic origin and methods of making such vectors are disclosed, the improved vectors comprising promoters and operators from λ phages and preferably do not include an active cro gene or an active N gene, the vectors having at least one endonuclease recognition site forcloning desired genes less than about 300 base pairs from the promoters and operators and being useful, as are methods utilizing the vectors, in producing a wide variety of prokaryotic, eukaryotic and viral polypeptides, hormones, enzymes, antigens, proteins and amino acids.
Inventors
- 👤 Walter Charles Fiers
- 👤 Erik Rene Remaut
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 16:00
CPC Classifications
C; C12; C12N; C12N15/00; C07; C07K; C07K14/00; C07K14/005; C07K14/435; C07K14/52; C07K14/555; C07K14/565; C12N15/09; C12N15/11; C12N15/52; C12N15/63; C12N15/70; C12N15/73; A; A61; A61K; A61K38/00; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/40; C07K2319/70; C07K2319/74; C07K2319/75; C12N2710/00; C12N2710/00011; C12N2710/22011; C12N2710/22022
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
46 words
A vector comprising at least one DNA sequence comprising at least one promoter and operator derived from bacteriophage X, characterized by at least one endonuclease recognition site located less than about 300 base pairs from that portion of said DNA sequence comprising said promoter and operator,
Claim 2
Dependent Claim
46 words
A vector comprising at least one DNA sequence comprising at least one promoter and operator derived from bacteriophage X, characterized by at least one endonuclease recognition site located less than about 300 base pairs from that portion of said DNA sequence comprising said promoter and operator,
Claim 3
Dependent Claim
17 words
The vector of claim 1, characterized by the absence of active cro gene or active N gene.
Claim 4
Dependent Claim
25 words
The vector of claim 1 or 2, characterized in that said promoter and operator is P L O L .or P R O R .
Claim 5
Dependent Claim
24 words
The vector of any one of claims 1 to 3, characterized in that said recognition site comprises EcoRI, BamHI, HindIII, PstI, Xba or Sal.
Claim 6
Dependent Claim
36 words
The vector of any one of claims 1 to 4, characterized in that said recognition site is located less than about 150 base pairs from that portion of said DNA sequence comprising said promoter and operator.
Claim 7
Dependent Claim
17 words
The vector of any one of claims 1 to 5, characterized by including a ribosome binding site.
Claim 8
Dependent Claim
18 words
The vector of claim 6, characterized in that said ribosome binding site is derived from bacteriophage MS2 replicase.
Claim 9
Dependent Claim
39 words
The vector of any one of claims 1 to 7, characterized by including in one of said endonuclease recognition sites a DNA sequence coding for a eukaryotic, prokaryotic or viral protein, polypeptide, enzyme, hormone, antigen or fragment thereof .
Claim 10
Independent Claim
72 words
A method for producing an improved cloning vector, characterized by inserting into an existing cloning vehicle at least one DNA sequence comprising at least one promoter and operator derived from bacteriophage λ and consisting of PROR or P L O L and providing in said cloning vehicle at least one endonuclease recognition site less than about 300 base pairs from that portion of said DNA sequence consisting of said promoter and operator.
Claim 11
Independent Claim
72 words
A method for producing an improved cloning vector, characterized by inserting into an existing cloning vehicle at least one DNA sequence comprising at least one promoter and operator derived from bacteriophage λ and consisting of PROR or P L O L and providing in said cloning vehicle at least one endonuclease recognition site less than about 300 base pairs from that portion of said DNA sequence consisting of said promoter and operator.
Claim 12
Dependent Claim
22 words
The method of claim 9, characterized by inactivating any active cro gene or any active N gene located in said DNA sequence.
Claim 13
Dependent Claim
21 words
The method of claim 9 or 10, characterized in that said recognition site comprises EcoRI, BamHI, HindIII, PstI, Xba or Sal.
Claim 14
Independent Claim
37 words
The method of any one of claims 9 to 11, characterized in that said recognition site is located less than about 150 base pairs from that portion of said DNA sequence consisting of said promoter and operator.
Claim 15
Independent Claim
20 words
The method of any one of claims 9 to 12, characterized by inserting a ribosome binding site in said vector.
Claim 16
Dependent Claim
18 words
The method of claim 13, characterized in that said ribosome binding site is derived from bacteriophage MS2 replicase.
Claim 17
Independent Claim
99 words
A method for producing a recombinant DNA molecule, characterized by inserting a DNA sequence coding for a eukaryotic, prokaryotic or viral protein, polypeptide, enzyme, hormone, antigen or fragment thereof into at least one endonuclease recognition site of a vector comprising (1) at least one DNA sequence comprising at least one promoter and operator derived from bacteriophage X and consisting of P R O R or P L O L and (2) said at least one endonuclease recognition site which is located less than about 300 base pairs from that portion of said DNA sequence comprising said promoter and operator.
Claim 18
Independent Claim
99 words
A method for producing a recombinant DNA molecule, characterized by inserting a DNA sequence coding for a eukaryotic, prokaryotic or viral protein, polypeptide, enzyme, hormone, antigen or fragment thereof into at least one endonuclease recognition site of a vector comprising (1) at least one DNA sequence comprising at least one promoter and operator derived from bacteriophage X and consisting of P R O R or P L O L and (2) said at least one endonuclease recognition site which is located less than about 300 base pairs from that portion of said DNA sequence comprising said promoter and operator.
Claim 19
Dependent Claim
23 words
A method for producing a polypeptide, characterized by culturing a host transformed with a vector according to claim 8, and collecting said polypeptide.
Claim 20
Dependent Claim
47 words
The method of claim 16, characterized in that said polypeptide comprises leukocyte interferon, fibroblast interferon, immune interferon, insulin, human growth hormone, animal growth hormone, antigens of hepatitis, foot and mouth disease and other viruses, and other prokaryotic, eukaryotic and viral enzymes, hormones, polypeptides, proteins or amino acids.
References Cited (Backward Citations)
No citation records indexed.
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