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US5180672A
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Ascorbate oxidase isolated from acremonium sp. hi-25 ferm bp-3124
Assignee: Asahi Kasei Corp
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Filing: 1991-02-06
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Granted: 1993-01-19
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Expires: 2011-02-06
Abstract
Abstract An ascorbate oxidase having an optimum pH range of 3.5-4.5 is isolated from Acremonium sp. Hi-25 BP-3124. The ascorbate oxidase is used in foods or drinks to oxidize ascorbate to prevent damage to the foods or drinks by oxidation.
Inventors
- 👤 Homare Itoh
- 👤 Takashi Shin
- 👤 Takahisa Yajima
- 👤 Shigeki Fukuyasu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 08:48
CPC Classifications
C; C12; C12Y; C12Y110/00; C12Y110/03; C12Y110/03003; C12N; C12N9/00; C12N9/0004; C12N9/0055; C12N9/0057; C12N9/0063; A; A23; A23B; A23B2/00; A23B2/70; A23B2/725; A23B2/729; A23B2/783; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
113 words
An isolated ascorbate oxidase having the properties as mentioned below: (1) having a pH optimum in the range of pH 3.5-4.5; (2) being activated by Fe ++ and inhibited by Cu ++ ; (3) having a molecular weight of 85,000±5,000 as determined by the gel filtration method, and subunit molecular weight of 23,000+2,000 as determined by the sodium dodecyl sulfate/polyacrylamide gel electrophoresis method; (4) having an isoelectric point of pI 4.0 as measured by the isoelectric focusing method; (5) showing stable activity at pH 8.0 when heated to a temperature as high as 60° C. for 30 minutes; and (6) showing stable activity at 4° C. over the pH range of pH 4-11.
Claim 2
Dependent Claim
113 words
An isolated ascorbate oxidase having the properties as mentioned below: (1) having a pH optimum in the range of pH 3.5-4.5; (2) being activated by Fe ++ and inhibited by Cu ++ ; (3) having a molecular weight of 85,000±5,000 as determined by the gel filtration method, and subunit molecular weight of 23,000+2,000 as determined by the sodium dodecyl sulfate/polyacrylamide gel electrophoresis method; (4) having an isoelectric point of pI 4.0 as measured by the isoelectric focusing method; (5) showing stable activity at pH 8.0 when heated to a temperature as high as 60° C. for 30 minutes; and (6) showing stable activity at 4° C. over the pH range of pH 4-11.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-01-19 | 1996-07-20 | 1997-01-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-01-19 | 2000-07-20 | 2001-01-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-01-19 | 2004-07-20 | 2005-01-19 | $7700.00 | Window Closed / Lapsed |