EP0442781A2
Expired / Lapsed
EP
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Ascorbate oxidase and its use
Assignee: Asahi Kasei Corp
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Filing: 1991-02-07
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Granted: —
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Expires: 2011-02-07
Abstract
Abstract Foods or drinks are maintained in the presence of but being ensured no direct contact with an oxidase which is able to express its ability in the acidic range and to oxidize ascorbate and substrates for the enzyme, thereby preventing damage of foods or drinks.
Inventors
- 👤 Homare Itoh
- 👤 Takashi Shin
- 👤 Takahisa Yajima
- 👤 Shigeki Fukuyasu
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 17:03
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0055; C12N9/0057; C12N9/0063; A; A23; A23B; A23B2/00; A23B2/70; A23B2/725; A23B2/729; A23B2/783; C12Y; C12Y110/00; C12Y110/03; C12Y110/03003; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
19 words
A novel ascorbate oxidase which expresses the optimum activity at least at acidic range of pH 3.5 - 4.5.
Claim 2
Dependent Claim
108 words
An ascorbate oxidase characterized by having the properties as mentioned below: (1) Optimum acting pH of 3.5 - 4.5; (2) being activated by Fe⁺⁺ and inhibited by Cu⁺⁺; (3) molecular weight: 85,000 ± 5,000 of molecular weight according to a gel filtration method, and 23,000 ± 2,000 of molecular weight subunit according to a sodium dodecyl sulfate/polyacrylamide gel electrophoresis method; (4) isoelectric point: pI 4.0 as measured by an isoelectric focusing method; (5) temperature stability: showing the stable activity at not higher than 60°C at pH 8.0 for 30 minutes; and (6) pH stability: showing the stable activity at 4°C within the range of pH 4 - 11.
Claim 3
Dependent Claim
39 words
A method for producing the ascorbate oxidase as mentioned in Claim 1, characterized by growing a microorganism which belongs to genus Acremonium and has the capability of producing ascorbate oxidase, and recovering the ascorbate oxidase from the culture medium.
Claim 4
Independent Claim
37 words
A method for preventing the oxidative deterioration of foods and drinks, characterized by adding, to a food or drink, an ascorbate oxidase with or without its substrates, thereby to effect the deoxygenation in the food or drink.
Claim 5
Independent Claim
45 words
A method for preventing the oxidative deterioration of foods and drinks, characterized by filling, into a sealed vessel, a food or drink, and an ascorbate oxidase with its substrates, in such a way that the enzyme and the substrates do not directly contact each other.
Claim 6
Dependent Claim
49 words
A deoxygenating agent characterized by that an ascorbate oxidase and its substrates are contained in a vessel which has an opening, or is at least partially constructed with a gas permeable material, in such a way that the enzyme and the substrates do not react each other before use.
References Cited (Backward Citations)
No citation records indexed.
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