US5178884A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Lyophilized and reconstituted red blood cell compositions
Assignee: Terumo BCT Inc
•
Filing: 1989-06-29
•
Granted: 1993-01-12
•
Expires: 2009-06-29
Abstract
Abstract A process and medium are disclosed for the lyophilization of red blood cells which comprises the use of solutions including monosaccharide hexoses and pentoses, and biocompatible amphipathic polymers to permit the reconstitution of viable red blood cells. Also disclosed are lyophilized and reconstituted erythrocyte and hemosome containing compositions.
Inventors
- 👤 Raymond P. Goodrich
- 👤 Christine M. Williams
- 👤 Roger W. Hackett
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 17:22
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/18
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
38 words
A transfusably-useful erythrocyte-containing and/or hemosome-containing composition reconstituted after lyophilization characterized by a P 50 in the range of about 26-28, an oxyhemoglobin content greater than about 90% of that of normal red blood cells, morphology,and normal ATP metabolism.
Claim 2
Dependent Claim
38 words
A transfusably-useful erythrocyte-containing and/or hemosome-containing composition reconstituted after lyophilization characterized by a P 50 in the range of about 26-28, an oxyhemoglobin content greater than about 90% of that of normal red blood cells, morphology,and normal ATP metabolism.
Claim 3
Dependent Claim
13 words
A composition according to claim 1 comprising erythrocytes having discocytic or stomatocytic morphology.
Claim 4
Dependent Claim
40 words
A composition according to claim 2 further comprising an additive comprising a polymer or mixture of polymers having a molecular weight of from about 1 K to about 360 K in a concentration of about 12 to 30% by weight.
Claim 5
Dependent Claim
11 words
A composition according to claim 3 wherein said polymers are amphipathic.
Claim 6
Dependent Claim
22 words
A composition according to claim 3 wherein said polymers have molecular weights in the range of about 2.5 K to 360 K.
Claim 7
Dependent Claim
24 words
A composition according to claim 3, 4 or 5 wherein said polymer is selected from the group consisting of polyvinylpyrrolidone, hydroxyethyl starch and dextran.
Claim 8
Dependent Claim
18 words
A composition according to claim 6 wherein said polymer comprises polyvinylpyrrolidone of average molecular weight of 40 K.
Claim 9
Dependent Claim
23 words
A composition according to claim 7 wherein said polyvinylpyrrolidone is present in said solution in a final concentration of about 14.4% by weight.
Claim 10
Dependent Claim
24 words
A composition according to claim 8 wherein said solution further comprises a monosaccharide in a final concentration of about 7.0 to 37.5% by weight.
Claim 11
Dependent Claim
26 words
A composition according to claim 2 wherein said composition further comprises a polyanion selected from the group consisting of inositol hexaphosphate, adenosine triphosphate, pyrophosphate and tripolyphosphate.
Claim 12
Dependent Claim
12 words
A composition according to claim 10 wherein said polyanion comprises inositol hexaphosphate.
Claim 13
Dependent Claim
23 words
A composition according to claim 2 further comprising polyethylene glycol of a molecular weight in a range of about 3 to 5 KD.
Claim 14
Dependent Claim
20 words
A lyophilized erythrocyte-containing and/or hemosome-containing composition characterized by a phosphorous-31 NMR lineshape characteristic of a bilayer in a gel phase.
Claim 15
Dependent Claim
20 words
A lyophilized erythrocyte-containing and/or hemosome-containing composition characterized by a phosphorous-31 NMR lineshape characteristic of a bilayer in a gel phase.
Claim 16
Dependent Claim
8 words
A composition according to claim 13 comprising erythrocytes.
Claim 17
Dependent Claim
40 words
A composition according to claim 14 further comprising an additive comprising a polymer or mixture of polymers having a molecular weight of from about 1 K to about 360 K in a concentration of about 12 to 30% by weight.
Claim 18
Dependent Claim
11 words
A composition according to claim 15 wherein said polymers are amphipathic.
Claim 19
Dependent Claim
22 words
A composition according to claim 15 wherein said polymers have molecular weights in the range of about 2.5 K to 360 K.
Claim 20
Dependent Claim
24 words
A composition according to claim 15, 16 or 17 wherein said polymer is selected from the group consisting of polyvinylpyrrolidone, hydroxyethyl starch and dextran.
Claim 21
Dependent Claim
18 words
A composition according to claim 18 wherein said polymer comprises polyvinylpyrrolidone of average molecular weight of 40 K.
Claim 22
Dependent Claim
13 words
A composition according to claim 14 wherein said solution further comprises a monosaccharide.
Claim 23
Dependent Claim
36 words
A composition according to claim 15, 16 or 17 further characterized by a phosphorous-31 NMR spectrum linewidth reduced by at least 8% as compared to the linewidth of a corresponding erythrocyte-containing composition lyophilized without said additive.
References Cited (Backward Citations)
US3347745A
Cited
US3344617A
Cited
DE3007913A1
Cited
US4572899A
Cited
WO1986006585A1
Cited
US4806343A
Cited
EP0342879B1
Cited
US4874690A
Cited
EP0356257A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-01-12 | 1996-07-13 | 1997-01-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-01-12 | 2000-07-13 | 2001-01-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-01-12 | 2004-07-13 | 2005-01-12 | $7700.00 | Window Closed / Lapsed |