Patent US1419795A successfully refreshed from Google Patents.
Patent US5175385A successfully refreshed from Google Patents.
US5175385A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Virus-resistant transgenic mice
Assignee: EDISON ANIMAL BIOTECHNOLOGY CENTER - OHIO UNIVERSITY WILSON HALL-WEST GREEN ATHENS OHIO 45701
•
Filing: 1987-09-03
•
Granted: 1992-12-29
•
Expires: 2007-09-03
Abstract
Abstract A transgenic mouse with enhanced viral resistance transmissible to its offspring is prepared by introduction of a gene encoding a human interferon having antiviral activity into a host mouse. The gene encodes the interferon of a different animal species and therefore its expression product is less toxic to the host at an embryonic, fetal, neonatal or junvenile stage of development. Preferably, the human beta interferon gene is introduced into the cells of a mouse.
Inventors
- 👤 Thomas E. Wagner
- 👤 Xiao-Zhuo Chen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 20:54
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/8509; A; A01; A01K; A01K67/00; A01K67/027; A01K67/0275; A01K67/0278; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/565; A01K2207/00; A01K2207/15; A01K2217/00; A01K2217/05; A01K2227/00; A01K2227/10; A01K2227/105; A01K2227/108; A01K2267/00; A01K2267/02
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
62 words
A transgenic mouse whose somatic and germ cells contain and express a gene coding for human beta interferon at a level sufficient to provide antiviral activity in said mouse, said gene having been introduced into said mouse or an ancestor of said mouse at an embryonic stage, and wherein said gene is operably linked to an at least partially constitutive non-interferon promoter.
Claim 2
Dependent Claim
62 words
A transgenic mouse whose somatic and germ cells contain and express a gene coding for human beta interferon at a level sufficient to provide antiviral activity in said mouse, said gene having been introduced into said mouse or an ancestor of said mouse at an embryonic stage, and wherein said gene is operably linked to an at least partially constitutive non-interferon promoter.
Claim 3
Dependent Claim
18 words
The mouse of claim 1, said mouse being fertile and capable of transmitting said gene to its offspring.
Claim 4
Dependent Claim
23 words
The mouse of claim 1 wherein said gene has been introduced into an ancestor of said mouse at an embryonic stage by microinjection.
Claim 5
Dependent Claim
20 words
The mouse of claim 1 wherein said gene has been introduced into said mouse at an embryonic stage by microinjection.
Claim 6
Dependent Claim
17 words
The mouse of claim 1 wherein said at least partially constitutive non-interferon promoter is the metallothionein promoter.
Claim 7
Dependent Claim
57 words
A method of producing a mouse of claim 1 which comprises: (a) providing a human beta interferon gene operably linked to an at least partially constitutive non-interferon promoter functional in a mouse, (b) introducing said gene into a mouse embryo, (c) transplanting said embryo into a pseudopregnant mouse, and (d) allowing said embryo to develop to term.
Claim 8
Dependent Claim
11 words
The method of claim 5 wherein said introducing is by microinjection.
Claim 9
Dependent Claim
17 words
The method of claim 5 wherein said at least partially constitutive non-interferon promoter is the metallothionein promoter.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-12-29 | 1996-06-29 | 1996-12-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-12-29 | 2000-06-29 | 2000-12-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-12-29 | 2004-06-29 | 2004-12-29 | $7700.00 | Window Closed / Lapsed |