US4497796A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Gene transfer in intact mammals
Assignee: University of California, San Diego
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Filing: 1982-12-08
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Granted: 1985-02-05
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Expires: 2002-12-08
Abstract
Abstract Methods and compositions are provided for gene transfer to intact mammals with expression of the exogenous genetic material in the host. Mammalian host cells which are regenerative, normally highly proliferative or subject to induced proliferation, are transformed or modified in vitro with DNA capable of replication and expression in the host cell, wherein the DNA becomes incorporated into the cell. The modified cells are found to regenerate in the host with expression of the introduced DNA. Particularly, mammalian cells were modified with genes providing for overproduction of a particular enzyme. The modified cells were reintroduced in the host under conditions providing for selective advantage of the modified cells.
Inventors
- 👤 Winston A. Salser
- 👤 Martin J. Cline
- 👤 Howard D. Stang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:29
CPC Classifications
C; C12; C12N; C12N15/00
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
129 words
A method for enhancing the genetic capability of a mammalian host by employing mammalian cells having exogenous genetic material capable of expression which comprises: isolating regenerative mammalian parent cells from a mammlian source compatible with said host; combining said regenerative mammalian parent cells with DNA including at least one gene capable of providing a selective advantage over the parent cell due to a naturally occurring deficiency or external stress inducement, said combining being under conditions where said DNA is introduced into said parent cell to produce modified cells; and introducing said modified cells into a compatible mammalian host under naturally occurring deficiency conditions or external stress induced conditions in said host providing said selective advantage, whereby said modified cells regenerate in said host at the source of said cells.
Claim 2
Independent Claim
129 words
A method for enhancing the genetic capability of a mammalian host by employing mammalian cells having exogenous genetic material capable of expression which comprises: isolating regenerative mammalian parent cells from a mammlian source compatible with said host; combining said regenerative mammalian parent cells with DNA including at least one gene capable of providing a selective advantage over the parent cell due to a naturally occurring deficiency or external stress inducement, said combining being under conditions where said DNA is introduced into said parent cell to produce modified cells; and introducing said modified cells into a compatible mammalian host under naturally occurring deficiency conditions or external stress induced conditions in said host providing said selective advantage, whereby said modified cells regenerate in said host at the source of said cells.
Claim 3
Dependent Claim
15 words
A method according to claim 1, wherein said parent cells are in the dividing phase.
Claim 4
Dependent Claim
17 words
A method according to claim 1, wherein said one gene providing a selective advantage expresses an enzyme.
Claim 5
Independent Claim
20 words
A method according to any of claims 1, 2 or 3, wherein said parent cells are bone marrow stem cells.
Claim 6
Dependent Claim
32 words
A method according to claim 3, wherein said enzyme is inhibited by a chemotherapeutic drug and said external stress induced conditions in said host comprise administering to said host said chemotherapeutic drug.
Claim 7
Dependent Claim
11 words
A method according to claim 5, wherein said drug is methotrexate.
Claim 8
Dependent Claim
18 words
A method according to claim 1, including the additional step of isolating said modified cells from said host.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-02-05 | 1988-08-06 | 1989-02-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-02-05 | 1992-08-06 | 1993-02-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-02-05 | 1996-08-06 | 1997-02-05 | $7700.00 | Window Closed / Lapsed |