US5162199A
Expired / Lapsed
US
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Neisseria-specific dna probe
Assignee: Roche Diagnostics GmbH
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Filing: 1990-05-31
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Granted: 1992-11-10
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Expires: 2010-05-31
Abstract
Abstract A Neisseria-specific DNA probe is chosen from the group of the oligonucleotides Opa 1, Opa 2, Opa 3, P III, Pil 1, IgA 1 and IgA 2 or from sequences complementary to these and it can have up to 5 further nucleotides at the 5' and/or 3' end. The probe is suitable for the detection of at least one of the pathogenic Neisseria species N. gonorrhoeae and N. meningitidis under hybridization conditions by the detection of the hybrid formation.
Inventors
- 👤 Anne Stern
- 👤 Karin Wolff
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 15:20
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6888; C12Q1/689; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/871
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
44 words
A Neisseria-specific DNA probe which specifically binds to pathogenic species of Neisseria and not to non-pathogenic species thereof and which is selected from the group of oligonucleotides consisting of Opa1, Opa 3, PIII, Pil1, IgA1, IgA2 and sequences complementary to each of said oligonucleotides.
Claim 2
Dependent Claim
44 words
A Neisseria-specific DNA probe which specifically binds to pathogenic species of Neisseria and not to non-pathogenic species thereof and which is selected from the group of oligonucleotides consisting of Opa1, Opa 3, PIII, Pil1, IgA1, IgA2 and sequences complementary to each of said oligonucleotides.
Claim 3
Dependent Claim
24 words
The probe according to claim 1, wherein said probe specifically binds to N. gonorrhoea and not to N. meningitidis and is the oligonucleotide IgA1.
Claim 4
Dependent Claim
24 words
The probe according to claim 1, wherein said probe specifically binds to N. meningitidis and not to N. gonorrhoea and is the oligonucletoide Opa3.
Claim 5
Dependent Claim
24 words
The probe according to claim 1, further having from 1 to 5 additional nucleotides at the 5' end and/or 3' end of said probe.
Claim 6
Dependent Claim
46 words
The probe according to claim 4, wherein said additional nucleotides correspond to the nucleotides at the 5' and/or 3' site in the natural gene from which the probes are derived from, said 5' and/or 3' site corresponding to the 5' and/or 3' end of said probe.
Claim 7
Dependent Claim
33 words
The probe according to claim 2 or 5, wherein said probe is selected from the group of oligonucleotides consisting of Opa1*, Opa3*, PIII*, Pil1*, IgA2* and sequences complementary to each of said oligonucleotides.
Claim 8
Dependent Claim
36 words
A Neisseria-specific DNA probe which specifically binds to the oligonucleotide N. gonorrhoea and not to the oligonucleotide N. meniningitidis and which is selected from the group of oligonucleotides consisting of Opa2and a sequence complementary to Opa2.
Claim 9
Dependent Claim
36 words
A Neisseria-specific DNA probe which specifically binds to the oligonucleotide N. gonorrhoea and not to the oligonucleotide N. meniningitidis and which is selected from the group of oligonucleotides consisting of Opa2and a sequence complementary to Opa2.
Claim 10
Dependent Claim
24 words
The probe according to claim 7, further having from 1 to 5 additional nucleotides at the 5' end and/or 3' end of said probe.
Claim 11
Dependent Claim
46 words
The probe according to claim 8, wherein said additional nucleotides correspond to the nucleotides at the 5' and/or 3' site in the neutral gene from which the probes are derived from, said 5' and/or 3' site corresponding to the 5' and/or 3' end of said probe.
Claim 12
Dependent Claim
29 words
The probe according to claim 8 or 9, wherein said probe is selected from the group of oligonucleotides consisting of Opa2* and a sequence complementary to the oligonucleotide Opa2*.
Claim 13
Dependent Claim
21 words
The probe according to any of claims 1 to 10, wherein said probe is contained in a single or double-stranded vector.
Claim 14
Dependent Claim
19 words
The probe according to any of claims 1 to 10, wherein said probe is present in a labelled form.
Claim 15
Independent Claim
49 words
A method for the detection of at least one of the pathogenic Neisseria species N. gonorrhoea and N. meningitidis using a hybridizing probe under hybridizing conditions and detection of hybrid formation, wherein a probe is used for the specific detection as claimed in any of claims 1 to 10.
Claim 16
Dependent Claim
38 words
The method according to claim 13, wherein at least one of the probes selected from the group of oligonucleotides consisting of PIII, Pil1, IgA2 and Opa1 is used for the combined detection of N. gonorrhoea and N. meningitidis.
Claim 17
Dependent Claim
38 words
The method according to claim 13, wherein at least one of the probes selected from the group of oligonucleotides consisting of PIII*, Pil1*, IgA2* and Opa1* is used for the combined detection of N. gonorrhoea and N. meningitidis.
Claim 18
Dependent Claim
27 words
The method according to claim 13, wherein at least one of the probes Opa2, IgA1, Opa2* and IgA1* is used for the sole detection of N. gonorrhoea.
Claim 19
Dependent Claim
30 words
The method according to claim 13, wherein the probe is selected from the group of oligonucleotides consisting of Opa3 and/or Opa3* is used for the sole detection of N. meningitidis.
Claim 20
Dependent Claim
18 words
The method according to claim 13, wherein the hybrid formation is detected via a labelling of the probe.
Claim 21
Independent Claim
81 words
A method for the detection of the presence or absence of at least one of the pathogenic Neisseria species N. gonorrhoea and N. meningitids is a sample comprising: adding to said sample at least one DNA probe selected from the group of oligonucleotides consisting of Opa1, Opa3, PIII, Pil1, IgA1, and IgA2 and sequences complementary to each of said oligonucleotides which specifically binds to pathogenic species of Neisseria and not to non-pathogenic species thereof, under hybridization conditions, and detecting hybrid formation.
Claim 22
Independent Claim
81 words
A method for the detection of the presence or absence of at least one of the pathogenic Neisseria species N. gonorrhoea and N. meningitids is a sample comprising: adding to said sample at least one DNA probe selected from the group of oligonucleotides consisting of Opa1, Opa3, PIII, Pil1, IgA1, and IgA2 and sequences complementary to each of said oligonucleotides which specifically binds to pathogenic species of Neisseria and not to non-pathogenic species thereof, under hybridization conditions, and detecting hybrid formation.
Claim 23
Dependent Claim
23 words
The method according to claim 19, wherein said probe is selected from the group of oligonucleotides consisting of PIII, Pil1, IgA2 and Opa1.
Claim 24
Dependent Claim
26 words
The method according to claim 19, wherein said probe has from 1 to 5 additional nucleotides at the 5' end and/or 3' end of said probe.
Claim 25
Dependent Claim
46 words
The method according to claim 21, wherein said additional nucleotides correspond to the nucleotides at the 5' and/or 3' site in the natural gene from which the probes are derived from, said 5' and/or 3' site corresponding to the 5' and/or 3' end of said probe.
Claim 26
Dependent Claim
32 words
The method according to claim 21 or 22, wherein said probe is selected from the group of oligonucleotides consisting of PIII*, Pil1*, IgA2*, Opa1* and sequences complementary to each of said oligonucleotides.
Claim 27
Independent Claim
71 words
A method for the specific detection of the presence or absence of N. gonorrhoea in a sample comprising: adding to said sample at least one DNA probe selected from the group of oligonucleotides consisting of IgA1, IgA1* and sequences complementary to each of said oligonuleotides which binds specifically to N. gonorrhoea and does not bind to N. meningitidis or to a non-pathogenic Neisseria species, under hybridization conditions, and detecting hybrid formation.
Claim 28
Independent Claim
71 words
A method for the specific detection of the presence or absence of N. gonorrhoea in a sample comprising: adding to said sample at least one DNA probe selected from the group of oligonucleotides consisting of IgA1, IgA1* and sequences complementary to each of said oligonuleotides which binds specifically to N. gonorrhoea and does not bind to N. meningitidis or to a non-pathogenic Neisseria species, under hybridization conditions, and detecting hybrid formation.
Claim 29
Independent Claim
71 words
A method for the specific detection of the presence or absence of N. meningitidis in a sample comprising: adding to said sample at least one DNA probe selected from the group of oligonucleotides consisting of Opa3, Opa3* and sequences complementary to each of said oligonucleotides which binds specifically to N. meningitidis and does not bind to N. gonorrhoea or to a non-pathogenic Neisseria species, under hybridization conditions, and detecting hybrid formation.
Claim 30
Independent Claim
71 words
A method for the specific detection of the presence or absence of N. meningitidis in a sample comprising: adding to said sample at least one DNA probe selected from the group of oligonucleotides consisting of Opa3, Opa3* and sequences complementary to each of said oligonucleotides which binds specifically to N. meningitidis and does not bind to N. gonorrhoea or to a non-pathogenic Neisseria species, under hybridization conditions, and detecting hybrid formation.
Claim 31
Independent Claim
73 words
A method for the specific detection of the presence or absence of N. gonorrhoea in a sample that does not contain a non-pathogenic Neisseria species comprising: adding to said sample at least on DNA probe selected from the group of oligonucleotides consisting of Opa2, Opa2* and sequences complementary to each of said oligonucleotides which binds specifically to N. gonorrhoea and does not bind to N. meningitidis, under hybridization conditions, and detecting hybrid formation.
Claim 32
Independent Claim
73 words
A method for the specific detection of the presence or absence of N. gonorrhoea in a sample that does not contain a non-pathogenic Neisseria species comprising: adding to said sample at least on DNA probe selected from the group of oligonucleotides consisting of Opa2, Opa2* and sequences complementary to each of said oligonucleotides which binds specifically to N. gonorrhoea and does not bind to N. meningitidis, under hybridization conditions, and detecting hybrid formation.
Claim 33
Independent Claim
21 words
The method according to any of claims 18 to 25, wherein said hybrid formation is detected via labelling of said probe.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-11-10 | 1996-05-11 | 1996-11-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-11-10 | 2000-05-11 | 2000-11-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-11-10 | 2004-05-11 | 2004-11-10 | $7700.00 | Window Closed / Lapsed |